Chemotactic Behavior of Pathogenic and Nonpathogenic Leptospira Species

Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France.
Applied and Environmental Microbiology (Impact Factor: 3.67). 12/2012; 78(23). DOI: 10.1128/AEM.02288-12


We have developed a capillary tube assay in combination with real-time PCR to quantitate the number of chemoattracted Leptospira cells. We identified Tween 80, glucose, sucrose, and pyruvate as attractants for Leptospira cells; amino acids and vitamin B12 were found to be nonchemotactic or weakly chemotactic. This assay has the general applicability to further our understanding
of leptospiral chemotaxis.

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    • "Accumulation of Leptospira cells near the agar drop To confirm the chemotactic behaviour of L. biflexa cells towards agar that contained an attractant, we first performed MAA using glucose and sucrose. Both these sugars are known to induce positive chemotactic responses in L. biflexa (Lambert et al., 2012b). When the agar contained no sugar, the cells did not accumulate near the agar drop (Fig. 2). "
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