Article

Increased vincristine production from Agrobacterium tumefaciens C58 induced shooty teratomas of Catharanthus roseus G. Don

Centre for Biotechnology, Institute of Science & Technology, JNT University, Hyderabad, AP, India.
Natural product research (Impact Factor: 0.92). 02/2009; 23(11):973-81. DOI: 10.1080/14786410802131153
Source: PubMed

ABSTRACT

Dimeric indole alkaloids are used extensively for cancer therapy. Agrobacterium tumefaciens C58 strain was used for induction of shooty teratoma in Catharanthus roseus using epicotyl and stem node explants. The transformed nature of shooty teratomas was confirmed by nopaline assay. Growth kinetics of shooty teratomas depicted maximum growth during 21-24 days of culture. Dimeric alkaloid vincristine in the transformed cultures was present at a concentration of 0.011 that was tenfold higher compared to untransformed control cultures.

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    • "A binary vector system in conjunction with the use of the Ri plasmid had been widely used for the integration of foreign genes into medicinal plants. The shooty teratomas of Catharanthus roseus epicotyls and stem nodal explants exhibited a 10-fold increased production of vincristine as compared to untransformed control cultures.[42] "
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    ABSTRACT: The wide diversity of plant secondary metabolites is largely used for the production of various pharmaceutical compounds. In vitro cell tissue or organ culture has been employed as a possible alternative to produce such industrial compounds. Tissue culture techniques provide continuous, reliable, and renewable source of valuable plant pharmaceuticals and might be used for the large-scale culture of the plant cells from which these secondary metabolites can be extracted. Alkaloids are one of the most important secondary metabolites known to play a vital role in various pharmaceutical applications leading to an increased commercial importance in recent years. The tissue culture techniques may be utilized to improve their production of alkaloids via somaclonal variations and genetic transformations. The focus of this review is toward the application of different tissue culture methods/techniques employed for the in vitro production of alkaloids with a systematic approach to improve their production.
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    • "Recently, increased vincristine production from Agrobacterium tumefaciens C58 induced shooty teratomas of C. roseus was observed (Begum et al. 2009). Authors noted that the dimeric alkaloid vincristine in the transformed cultures was present at a concentration of 0.011 that was tenfold higher compared to untransformed control cultures. "
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