Article

Cytogenetical damage in exfoliated oral mucosa cells in elderly people suffering denture stomatitis

Department of Dental Clinics, University of Sacred Heart, USC, Bauru, SP, Brazil.
Gerodontology (Impact Factor: 1.09). 06/2009; 27(3):183-8. DOI: 10.1111/j.1741-2358.2009.00315.x
Source: PubMed

ABSTRACT

The aim of this study was to evaluate comparatively the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from chronic denture stomatitis patients and healthy controls.
Over the course of ageing, individuals may develop many diseases such as denture stomatitis.
A total of 23 chronic denture stomatitis patients and 23 controls presenting good oral conditions were included in this study. Individuals had epithelial cells mechanically exfoliated, placed in fixative and placed on clean slides, which were checked for nuclear phenotypes.
The results indicated no statistically significant differences (p > 0.05) of micronucleated oral mucosa cells from chronic denture stomatitis patients when compared to healthy controls. Nevertheless, chronic denture stomatitis was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis as depicted by significant differences (p < 0.05) between groups. No interaction was observed between smoking and chronic denture stomatitis.
In summary, these data indicated that chronic denture stomatitis was able to induce cytotoxic effects as assessed by a micronucleus test.

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    • "By eliminating cells with genetic alterations, which may include genes engaged in DNA repair and/or in the control of cell proliferation and differentiation (proto-oncogenes and tumor suppressors), apoptosis constitutes a protective mechanism against cancer resulting from genetic and/or chromosomal mutations involving such genes [9]. By constantly renewing tissues , apoptosis ensures tissue homeostasis; however, its occurrence in increased frequencies can be indicative of genotoxic effects that are related to the initiation of a malignant transformation process [10] [11] [12] [13]. Few studies have been conducted aiming to investigate the association between periodontal disease and occurrence of micronuclei and nuclear changes indicative of apoptosis [14] [15] [16] [17]. "
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