Diplococcium variegatum , a new conidial fungus from the semi-arid Caatinga biome of Brazil

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DOI: 10.5248/127.59
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Abstract
Diplococcium variegatum sp. nov., found on a decaying leaf of an unidentified plant, is described and illustrated. It is characterized by variegated pigmented structures and limoniform to ovoid, 0-septate, pale brown, brown to dark brown conidia in acropetal chains.
ISSN (print) 0093-4666 © 2014. Mycotaxon, Ltd. ISSN (online) 2154-8889
MYCOTAXON
http://dx.doi.org/./.
Volume 127, pp. 59–62 January–March 2014
Diplococcium variegatum, a new conidial fungus
from the semi-arid Caatinga biome of Brazil
S S  S, A C R  C,
L F P G*,  R F. C-R
1Universidade Estadual de Feira de Santana,
Departamento de Ciências Biológicas, Laboratório de Micologia,
Avenida Transnordestina, s/n, Novo Horizonte, 44036-900, Feira de Santana, BA, Brazil.
2Instituto de Investigaciones Fundamentales en Agricultura Tropical ‘Alejandro de Humboldt’
(INIFAT), Académico Titular de la Academia de Ciencias de Cuba,
Calle 1 Esq. 2, Santiago de Las Vegas, C. Habana, Cuba, C.P. 17200
*C : lgusmao@uefs.br
A — Diplococcium variegatum sp. nov., found on a decaying leaf of an unidentied
plant, is described and illustrated. It is characterized by variegated pigmented structures and
limoniform to ovoid, 0-septate, pale brown, brown to dark brown conidia in acropetal chains.
K  — hyphomycetes, plant debris, taxonomy
Introduction
During surveys for conidial fungi on plant debris in a semi-arid region of
northeast Brazil, an interesting specimen of Diplococcium Grove was found.
e fungus clearly diers morphologically from all described species and is
therefore described as new.
Materials & methods
Expeditions to Serra da Jibóia, Bahia, Brazil were made every 3 months, from
October 2008 to April 2009. Samples of plant debris (twigs, bark, leaves, and petioles)
were collected in paper bags. In the laboratory the samples were placed in Petri dish
moist chambers and stored in a 170 L polystyrene box with 200 mL sterile water plus 2
mL glycerol at 25°C for 30 days (Castañeda-Ruiz 2005). During this period, the samples
were examined for the presence of conidial fungi. Slides were prepared in polyvinyl
alcohol plus lactic acid and phenol. Measurements were made at a magnication of
×1000. Micrographs were obtained with an Olympus BX 51.
60 ... Silva & al.
Taxonomy
Diplococcium variegatum S.S. Silva, Gusmão & R.F. Castañeda, sp. nov. F. 1
MB MB 
Diers from Diplococcium parcum by larger limoniform to ovoid conidia and the
variably colored conidiogenous cells.
T: Brazil. Bahia State: Santa Teresinha, Serra da Jibóia, on decaying leaves of an
unidentied plant, 19.II.2009; coll. S.S. Silva. (Holotype: HUEFS 194254).
E: Latin, variegatum, referring to the variable coloration of the conidiogenous
cells and conidia.
C euse, hairy, brown. Mycelium supercial and immersed in
substrate. C macronematous, mononematous, erect, straight
to slightly exuous, simple or branched, 4−7-septate, smooth, brown below,
variegated pigmented towards the apex, 135−189 × 12.0−16.5 µm, cell basal
lobed 21−45 µm wide. C  polytretic, integrated, terminal
and intercalary, pale brown to dark brown to reddish brown, 19.5−25 × 5.0−6.5
µm. C limoniform to ovoid, unicellular, smooth, thick-walled, pale
brown to dark brown or reddish brown, 7.5−22.5 × 5−12 µm, forming acropetal
chains. Teleomorph unknown.
N: Diplococcium was described by Grove (1885) with the type species
D. spicatum Grove from dead wood in England. Braun et al. (1996), Castañeda-
Ruiz & Kendrick (1991), Cruz et al. (2007), Goh & Hyde (1998), Hernández-
Restrepo et al. (2012), Ma et al. (2012), Pirozynski (1972), and Wang &
Sutton (1998) documented 28 accepted species of Diplococcium, which are
characterized by terminal or intercalary polytretic conidiogenous cells that
produce 0–7-septate conidia in acropetal chains. Diplococcium capitatum Piroz.,
D. hughesii C.J.K. Wang & B. Sutton, and D. parcum Hol.-Jech. are also species
that produce exclusively aseptate conidia. Diplococcium capitatum diers from
D. variegatum by conidiogenous pores conned to a swollen terminal cell
that produces short cylindric or broadly ellipsoid conidia (Pirozynski 1972);
D. hughesii is separated by its subglobose oval to oblong smaller (7–12 ×
5–7µm) conidia and highly branched longer conidiophores (Holubová-Jechová
1982, Wang & Sutton 1998), while D. parcum produces smaller (8.5–13 × 5.5–8
µm) ovate to obpyriform or ellipsoidal conidia (Holubová-Jechová 1982).
Acknowledgments
e authors express their sincere gratitude to Dr. De-Wei Li and Prof. Dr. Bryce
Kendrick for their critical review of the manuscript. e authors thank the Program
of Research on Biodiversity in the Brazilian semi-arid (PPBIO semi-arid/ Ministry
of Technology and Science). RFCR is grateful to the Cuban Ministry of Agriculture
and “Programa de Salud Animal y Vegetal,” project P131LH003033 Cuban Ministry of
Science, Technology and Environment for facilities.
Diplococcium variegatum sp. nov. (Brazil) ... 61
F 1. Diplococcium variegatum (ex HUEFS 194254). A. Conidiophore and conidia. B. Conidio-
genous cell and pores (indicated by arrows). C. Conidiogenous cells and conidia. D–F. Conidia.
Scale bars: A = 20 µm; B–F = 5 µm.
Literature cited
Braun U, Hosagoudar VB, Abraham TK. 1996. Diplococcium atrovelutinum sp. nov. from India.
New Botanist 23: 1–4.
Castañeda-Ruiz RF. 2005. Metodología en el estudio de los hongos anamorfos. Anais do V
Congresso Latino Americano de Micología, Brasilia: 182–183.
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Castañeda-Ruiz RF, Kendrick B. 1991. Ninety conidial fungi from Cuba and three from Canada.
University of Waterloo, Biology Series 35: 1–132.
Cruz ACR, Gusmão LFP, Leão-Ferreira SM, Castañeda-Ruiz RF. 2007. Conidial fungi from the
semi-arid Caatinga biome of Brazil. Diplococcium verruculosum sp. nov. and Lobatopedis
longirostratum sp. nov. Mycotaxon 103: 33–38.
Goh TK, Hyde KD. 1998. A synopsis of and a key to Diplococcium species, based on the literature,
with a description of a new species. Fungal Diversity 1: 65–83.
Grove WB. 1885. New or noteworthy fungi. Part II. J. Bot. 23: 161–169.
Hernández-Restrepo M, Silvera-Simón C, Mena-Portales J, Mercado-Sierra Á, Guarro J, Gené J.
2012. ree new species and a new record of Diplococcium from plant debris in Spain. Mycol.
Prog. 11: 191–199. http://dx.doi.org/10.1007/s11557-011-0741-6
Holubová-Jechová V. 1982. Lignicolous hyphomycetes from Czechoslovakia 6. Spadicoides and
Diplococcium. Folia Geobot. Phytotax. 17: 295–327.
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new hyphomycetes on dead branches from Fujian Province, China. Mycoscience 53: 25–30.
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