Isolation of Candida dubliniensis from denture wearers

Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
Journal of Medical Microbiology (Impact Factor: 2.25). 08/2009; 58(Pt 7):959-62. DOI: 10.1099/jmm.0.008391-0
Source: PubMed


Candida albicans is considered the most important Candida species able to cause oral infections in denture wearers. In recent years, Candida dubliniensis has emerged as a pathogenic yeast in humans. The close phenotypic similarities of C. albicans and C. dubliniensis have led to the misidentification of these species. In this work, our aim was to verify through PCR the presence of C. dubliniensis in palate and maxillary denture samples from 112 denture wearers presenting with or without denture-related stomatitis (DRS). C. dubliniensis was isolated at low rates from both palate (5.3 % and 10.7 %) and maxillary denture (5.3 % and 8.9 %) samples from wearers regardless of the presence of the disease. However, when C. dubliniensis was detected in individuals with DRS, it was always associated with C. albicans. In addition, our results showed that C. albicans was the most commonly identified candidal species in maxillary denture and hard palate samples from DRS patients (78.5 % and 89.2 %, respectively) as well as from controls (31.2 % and 28.5 %, respectively). In conclusion, C. dubliniensis was detected in the oral environment of denture wearers. The association of C. dubliniensis with C. albicans occurred in approximately 10 % of the DRS cases.

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    • "In this study, monocytes from elderly denture wearers with Candida-associated denture stomatitis (DS) presented immunomodulatory aspects not observed among the elderly without the disease, although they were denture wearers. These findings led us to speculate whether inherent aspects not associated with age, could result in individual predisposition to DS, in the presence of local denture-related factors, including Candida spp. in biofilm covering the denture inner surface10111222,48]. Indeed, aged populations undergo physiological changes such as decreased salivary flow and aspects related to immunosenescence that make them more susceptible to the development of DS[20,49]. "
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    ABSTRACT: Candida-associated denture stomatitis (DS) is the most frequent lesion among denture wearers, especially the elderly. DS is strongly associated with Candida albicans, as well as local and systemic factors, such as impaired immune response. Monocytes are important in the protective immune response against the fungus by the production of cytokines that recruit and activate leukocytes. There are functional changes in these cells with age, and individual alterations involving monocyte response may predispose the host to developing infections by Candida spp. In this study, our aim was to evaluate the production of TNF-α, IL-6, CXCL8, IL-1β, MCP-1 and IL-10 by monocytes from elderly denture wearers with/without DS and elderly or young non-denture wearers. We detected that monocytes from elderly denture wearers with Candida-related denture stomatitis produced lower levels of CXCL-8, IL-6 and MCP-1. This imbalance in cytokine levels was observed in spontaneous or LPS-stimulated production. Therefore, our data suggested that inherent aspects of the host, such as changes in cytokine production by monocytes, might be associated with the development and the persistence of DS irrespective of aging.
    Full-text · Article · Jan 2016 · Cytokine
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    • "Whilst some phenotypic tests generated ambiguous results, 'gold-standard' DNA analysis by PCR amplification methods based on differences in the sequences of HWP1 and topoisomerase II genes, and sequence analysis of the D1/D2 domain of the large subunit rRNA gene, permitted the definitive identification of the nine isolates as Candida dubliniensis. Other authors have previously reported the specificity and suitability of these methods to identify clinical isolates of C. dubliniensis (BoscoBorgeat et al., 2011; Gasparoto et al., 2009; Khlif et al., 2009; Marcos-Arias et al., 2009; Sahand et al., 2009). Clearly, some phenotypic tests accurately identified the unusual isolates correctly. "
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    ABSTRACT: Candida dubliniensis is a pathogenic yeast of the genus Candida closely related to Candida albicans. The phenotypic similarity of these two species often leads to misidentification of C. dubliniensis isolates in clinical samples. DNA-based methods continue to be the most effective means of discriminating accurately between the two species. Here we report on the identification of nine unusual Candida isolates that showed ambiguous identification patterns on the basis of their phenotypic and immunological traits. The isolates were categorized into two groups. Group I isolates were unable to produce germ tubes and chlamydospores and to agglutinate commercial latex particles coated with a monoclonal antibody highly specific for C. dubliniensis. Group II isolates grew as pink and white colonies on CHROMagarTM Candida and ChromIDTM Candida respectively. Carbohydrate assimilation profiles obtained with API ID 32C® together with PCR amplification with specific primers and DNA sequencing allowed a reliable identification of the nine unusual clinical isolates as Candida dubliniensis. Copyright © 2014, the Society for General Microbiology.
    Full-text · Article · Dec 2014 · Journal of Medical Microbiology
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    • "DS is an inflammatory lesion of the palatal mucosa under complete or partial removable dentures and affects up to 65% of denture wearers. There are various factors that influence the onset and severity of DS: denture trauma, continuous denture wearing, salivary flow, denture cleanliness , denture base material, denture age, cellular immunity, smoking, dietary factors, pH of the denture plaque and oral microbiota (Coco et al., 2008; Gasparoto et al., 2009). "
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    ABSTRACT: The aim of this study was to isolate, quantify, identify, and compare opportunistic microorganisms (Candida and Staphylococcus genera and Enterobacteriaceae/Pseudomonadaceae families) from prosthesis-fitting surfaces, the hard palate, and mouth rinses of individuals wearing removable maxillary prosthesis with (50) and without (50) lesions of denture stomatitis (DS). The strains were collected and identified using phenotypic, biochemical and molecular tests. The counts of microorganisms were significantly higher in the group of individuals with DS (P < 0.05). C. albicans was the most frequently isolated yeast species in both groups, following by C. tropicalis and C. glabrata. Six isolates were identified as C. dubliniensis. S. aureus and S. epidermidis were the most frequent Staphylococcus species in both groups. Klebsiella pneumoniae was the predominant species in both groups. The association between Candida spp. and bacteria isolated in this study with DS suggests that these microorganisms may play important roles in the establishment and persistence of this disease.
    Full-text · Article · Jun 2013 · Diagnostic microbiology and infectious disease
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