Small-Angle Neutron Scattering for Molecular Biology: Basics and Instrumentation

Center for Structural Molecular Biology, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
Methods in Molecular Biology (Impact Factor: 1.29). 02/2009; 544:293-305. DOI: 10.1007/978-1-59745-483-4_19
Source: PubMed


As researchers strive to understand the interplay between the complex molecular systems that make up living cells, tools for characterizing the interactions between the various players involved have developed. Small-angle neutron scattering (SANS) plays an important role in building a molecular-level understanding of the structures of macromolecular systems that make up cells. SANS is widely applicable to the study of biological structures including, but by no means limited to, protein-protein or protein-nucleic acid complexes, lipid membranes, cellular scaffolding, and amyloid plaques. Here, we present a brief description of the technique as it is commonly applied to the study of biological systems and an overview instrumentation that is available at the various facilities around the world.

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