Frequency and function of circulating invariant NKT cells in autoimmune diabetes mellitus and thyroid diseases in Colombian patients

Group of Immunovirology, University of Antioquia, Medellin, Colombia.
Human immunology (Impact Factor: 2.14). 05/2009; 70(4):262-8. DOI: 10.1016/j.humimm.2009.01.012
Source: PubMed


The frequency and functionality of peripheral blood invariant (iNKT) cells and their subsets, as well as other regulatory T-cell subsets, were evaluated in patients with type 1A diabetes mellitus (DM1), Hashimoto's disease, and Graves' disease. In addition to healthy individuals (HC), patients with type 2 diabetes mellitus (DM2) were included as controls because this disease has a different physiopathology. A similar frequency of total iNKT cells, as well as their subsets, existed among HC and the different study groups. Similar results were reported when we compared the frequency of CD4(+)/CD25(high) T cells, CD8(+)/CD28(negative) T cells, and gamma-delta T cells among HC and study groups, whereas patients with DM2 exhibited a higher frequency of CD8(+)/CD28(negative) T cells compared with HC and DM1. Also, patients with DM2 exhibited a lower frequency of CD4(negative) and CD4(+) iNKT cells expressing tumor necrosis factor-alpha (TNF-alpha) than HC. We did not observe significant differences in the frequency of iNKT cells expressing interleukin-4 or interferon-gamma among study groups and controls. Our findings support a normal frequency and function of peripheral blood iNKT cells in different endocrine autoimmune diseases, but an abnormal expression of TNF-alpha by circulating iNKT cells from patients with DM2.

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Available from: Alejandro Roman-Gonzalez, Dec 15, 2014
    • "IL-4 has important role in immune cell chemotaxis, formation of endothelial cell adhesion molecules and has numerous anti-inflammatory effects which prevent the complications of atherosclerosis, the primary cause of coronary heart disease. In type 2 diabetes the frequency of invariant natural killer T cells expressing IL-4 did not significantly differ from that of healthy subjects.[9] IL-12 has been identified as a pro-inflammatory cytokine which is thought to contribute to the development of atherosclerosis. "
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    ABSTRACT: Contradictory reports about the role of cytokines, particularly interleukins (IL) in atherosclerosis are found in the literature. This study was aimed to investigate the association between the levels of cytokines notably IL-4, IL-12, IL-18 and, the atherogenicity and glycemic control in patients with type 2 diabetes mellitus. Seventy five patients with type 2 diabetes mellitus (25 males and 50 females) attending diabetic clinic during 1st August 2008 to 30th December 2009 as well as seventy healthy subjects (38 males and 32 female) were enrolled in the study. Fasting serum lipid profile and IL-4, IL-12 and IL-18 levels were determined. The serum lipid profile of diabetic patients was significantly different from healthy subjects, favoring atherogenicity. IL 4, 12, and 18 were significantly higher in diabetic patients compared with healthy subjects. Significant association of high serum IL-18 with poor glycemic control (P < 0.001) assessed by HbA1c, long duration of diabetes and atherogenic index were observed. IL-18 can serve as a predictor for pre-clinical atherosclerosis and poor glycemic control in type 2 diabetes mellitus.
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    • "However, none of further studies using different approaches for the detection of iNKT cells confirmed the results of Wilson and Kukreja. At least in the peripheral blood of T1DM patients, the proportion of iNKT cells is not reduced [67] [68] [69] [70] [71] [72] [73]. More importantly , Berzins and his colleagues addressed the simple question regarding the reciprocation of iNKT cell characteristics in peripheral blood versus those in visceral organs [74]. "
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    ABSTRACT: iNKT cells, CD1d dependent natural killer T cells are a unique population of T cells. The capacity of iNKT cells to produce regulatory cytokines first provided an indication of their regulatory potential. Later on, in experimental models as well as in patients afflicted with an auto-immune disease, such as Type 1 diabetes mellitus, multiple sclerosis, and systemic lupus erythematosus along with others, a deficit in iNKT cell number was observed, suggesting the role these cells may possibly have in the prevention of auto-immune diseases. More importantly, experimental strategies which focused on increasing the volume or stimulation of iNKT cells in laboratory animals, demonstrated an improved level of protection against the development of auto-immune diseases. This article reviews the mechanism of protection against autoimmunity by iNKT cells, discusses the obstacles against and indications for the potential use of iNKT cell manipulation in the treatment of human auto-immune diseases.
    Full-text · Article · Mar 2011 · Cytokine
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    No preview · Conference Paper · Oct 1996
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