Nonlinear optical contrast enhancement for optical coherence tomography

Optics Express (Impact Factor: 3.49). 02/2004; 12(2):331-41. DOI: 10.1364/OPEX.12.000331
Source: arXiv


We present a new interferometric technique for measuring Coherent Anti-Stokes Raman Scattering (CARS) and Second Harmonic Generation (SHG) signals. Heterodyne detection is employed to increase the sensitivity in both CARS and SHG signal detection, which can also be extended to different coherent processes. The exploitation of the mentioned optical nonlinearities for molecular contrast enhancement in Optical Coherence Tomography (OCT) is presented.

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Available from: Claudio Vinegoni, Mar 25, 2014
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    • "The main extensions enable the OCT Doppler analysis (D-OCT) [13], polarization sensitive (PS-OCT) [14] [15] [16] [17] [18] [19] [20] or expand the OCT measurements by wavelength dependent analysis of the device scattering or absorption features (SOCT) [21]. The other functional OCT systems which cannot be omitted, are Coherent Anti-Stokes Raman Scattering OCT (CARS-OCT) [22] [23] and Coherent Stokes Raman Scattering OCT (CSRS-OCT) [23], Second Harmonic Generation OCT (SHG-OCT) [23], and Third Harmonic Generation OCT (THG-OCT) [23]. Our research interests are concentrated on polarization-sensitive OCT systems. "
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    ABSTRACT: Optical Coherence Tomography (OCT) is one of the most rapidly advancing techniques. This method is capable of non-contact and non-destructive investigation of the inner structure of a broad range of materials. Compared with other methods which belong to the NDE/NDT group (Non-Destructive Evaluation/Non-Destructive Testing methods), OCT is capable of a broad range of scattering material structure visualization. Such a non-invasive and versatile method is very demanded by the industry. The authors applied the OCT method to examine the corrosion process in metal samples coated by polymer films. The main aim of the research was the evaluation of the anti-corrosion protective coatings using the OCT method. The tested samples were exposed to a harsh environment. The OCT measurements have been taken at different stages of the samples degradation. The research and tests results have been presented, as well as a brief discussion has been carried out.
    Full-text · Article · Jan 2012 · Metrology and Measurement Systems
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    • "To overcome this, multiplex CARS [21], [22] probes multiple modes simultaneously by the use of picosecond pump and femtosecond Stokes pulses, but the nonresonant background distorts the vibrational spectra. A recent important method to reject the background was to take advantage of the coherent properties of CARS signals and perform interferometric detection [23]–[30]. In this scheme, the anti-Stokes pulses from the sample are mixed with pulses in the same frequency range from a local oscillator, resulting in phase-sensitive measurements. "
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    ABSTRACT: Vibrational contrast imaging of the distribution of complex biological molecules requires the use of techniques that provide broadband spectra with sufficient resolution. Coherent anti-Stokes Raman scattering (CARS) microscopy is currently limited in meeting these requirements due to the presence of a nonresonant background and its inability to target multiple resonances simultaneously. We present nonlinear interferometric vibrational imaging (NIVI), a technique based on CARS that uses femtosecond pump and Stokes pulses to retrieve broadband vibrational spectra over 200 cm<sup>-1</sup> (full-width at half maximum). By chirping the pump and performing spectral interferometric detection, the anti-Stokes pulses are resolved in time. This phase-sensitive detection allows suppression of not only the nonresonant background, but also of the real part of the nonlinear susceptibility χ<sup>(3)</sup>, improving the spectral resolution and features to make them comparable to those acquired with spontaneous Raman microscopy, as shown for a material sample and mammary tissue.
    Full-text · Article · Sep 2010 · IEEE Journal of Selected Topics in Quantum Electronics
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    • "Therefore, it is well suited for high-resolution imaging of tissue microstructures, as well as tissue functions based on the scattering (such as Doppler OCT [120]–[123]) or birefringence (such as polarization-sensitive OCT [124]–[128]). Different approaches have been pursued to enhance the scattering contrast through exogenous contrast agents [129]–[138], or exploit other contrast mechanisms including absorption [28], [139]–[143], fluorescence [38]–[40], second harmonics [112], [144], [145], and CARS [118], [119]. An alternative approach to obtain comprehensive information from tissue imaging is to combine OCT with other imaging modalities based on complementary contrast mechanisms. "
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    ABSTRACT: We developed a combined optical coherence tomography (OCT) and fluorescence laminar optical tomography (FLOT) system for co-registered depth-resolved structural and molecular imaging. Experimental results using a mouse model with human breast cancer xenograft are presented.
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