Yoshimoto, T., Yasuda, K., Tanaka, H., Nakahira, M., Imai, Y., Fujimori, Y. et al. Basophils contribute to T(H)2-IgE responses in vivo via IL-4 production and presentation of peptide-MHC class II complexes to CD4+ T cells. Nat. Immunol. 10, 706-712

Department of Immunology and Medical Zoology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
Nature Immunology (Impact Factor: 20). 08/2009; 10(7):706-12. DOI: 10.1038/ni.1737
Source: PubMed


Basophils express major histocompatibility complex class II, CD80 and CD86 and produce interleukin 4 (IL-4) in various conditions. Here we show that when incubated with IL-3 and antigen or complexes of antigen and immunoglobulin E (IgE), basophils internalized, processed and presented antigen as complexes of peptide and major histocompatibility complex class II and produced IL-4. Intravenous administration of ovalbumin-pulsed basophils into naive mice 'preferentially' induced the development of naive ovalbumin-specific CD4+ T cells into T helper type 2 (T(H)2) cells. Mice immunized in this way, when challenged by intravenous administration of ovalbumin, promptly produced ovalbumin-specific IgG1 and IgE. Finally, intravenous administration of IgE complexes rapidly induced T(H)2 cells only in the presence of endogenous basophils, which suggests that basophils are potent antigen-presenting cells that 'preferentially' augment T(H)2-IgE responses by capturing IgE complex.

Download full-text


Available from: Yoshihiro Fujimori, Mar 11, 2014
  • Source
    • "Most of these crucial questions have largely been investigated in mouse models of either allergy or parasitic infections.In 2009, Sokol et al. demonstratedthat the absence of major antigen presenting cells did not aect the 2 immunity whereas basophils expressed major histocompatibility complex (MHC)-II molecules and were able to process and present antigens to T cells by migrating to the lymph nodes[43]. Similar functions were discovered at the same time byPerrigoue et al. and Yoshimoto et al. who bothshowed that basophils crucially initiated 2-type immune responses to nematodes[44,45]. Sokol et al. also reported that mouse basophils respond to innate triggering by protease allergens, such as papain , which results in the crucial early supply of IL-4 required for 2 development[43]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: In recent years there has been growing evidence to suggest a major role for basophils in allergy despite being one of the rarest leukocytes. These blood- borne cells have clearly been observed to invade other tissues such as skin or lung affected by allergic inflammation. More recent studies have shown that basophil responses - Typified by degranulation - correlate with the severity of allergic reactions in food allergy. However, despite studies suggesting major input in governing allergy symptoms their immuno modulatory roles are less clearly defined. While basophils are an important early source for pro-allergic Th2-type cytokines, interleukins 4 and 13 (IL-4 and IL-13), it is unclear whether they play a role in initiating allergic immunity or merely enhance existing allergy. In this regard, mouse models of allergy are more supportive of a key innate immune function of basophils in orchestrating Th2- Type immunity and allergy, and potentially acting as antigen presenting cells. However, these import-ant roles have not been observed in primary human basophils although there is clear evidence to suggest that they crucially promote group 2 innate lymphoid cells (ILC2s) in atopic dermatitis. Shedding more light on these immunomodulatory roles of human basophils, and differentiating them from their rodent counterparts, will provide much needed greater understanding of this elusive cell type, especially in view of developing new therapeutic strategies against allergy.
    Preview · Article · Aug 2015 · Allergo Journal International
  • Source
    • "Basophils are IgE-activated granulocytes that, unlike tissue-resident mast cells, circulate in the blood. They play a critical role in the IgE-mediated development of chronic allergic reactions and inflammation [97] [98], and they can also promote polarization towards Th2 responses by IgE-independent antigen presentation in mice [99] [100]. Basophils are recruited to a tick-feeding site and accumulate in the host skin during second and consequent (but not primary) tick infestation, where they act as important tick rejection factors [101] [102]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Next generation sequencing and proteomics have helped to comprehensively characterize gene expression in tick salivary glands at both the transcriptome and the proteome level. Functional data are, however, lacking. Given that tick salivary secretions are critical to the success of the tick transmission lifecycle and, as a consequence, for host colonization by the pathogens they spread, we thoroughly review here the literature on the known interactions between tick saliva (or tick salivary gland extracts) and the innate and adaptive vertebrate immune system. The information is intended to serve as a reference for functional characterization of the numerous genes and proteins expressed in tick salivary glands with an ultimate goal to develop novel vector and pathogen control strategies. We overview all the known interactions of tick saliva with the vertebrate immune system. The provided information is important, given the recent developments in high-throughput transcriptomic and proteomic analysis of gene expression in tick salivary glands, since it may serve as a guideline for the functional characterization of the numerous newly-discovered genes expressed in tick salivary glands. Copyright © 2015. Published by Elsevier B.V.
    Full-text · Article · Jul 2015 · Journal of proteomics
    • "Likewise, GM-CSF amplifies IL-3-induced differentiation of basophils from bone marrow cells [30], suggesting a possible role as a growth factor for basophil precursors. Furthermore, basophils have been shown to express MHC-II and co-stimulatory molecules and act as potent antigen presenting cells (APCs) to stimulate TH2 type responses both in vitro [31] and in vivo [32]. It is apparent that GM-CSF has a more profound role in the modulation of immune responses beyond its role as a CSF, something that has remained under appreciated. "
    [Show abstract] [Hide abstract]
    ABSTRACT: GM-CSF was originally identified as a colony stimulating factor (CSF) because of its ability to induce granulocyte and macrophage populations from precursor cells. Multiple studies have demonstrated that GM-CSF is also an immune-modulatory cytokine, capable of affecting not only the phenotype of myeloid lineage cells, but also T-cell activation through various myeloid intermediaries. This property has been implicated in the sustenance of several autoimmune diseases like arthritis and multiple sclerosis. In contrast, several studies using animal models have shown that GM-CSF is also capable of suppressing many autoimmune diseases such as Crohn's disease, Type-1 diabetes, Myasthenia gravis and experimental autoimmune thyroiditis. Knockout mouse studies have suggested that the role of GM-CSF in maintaining granulocyte and macrophage populations in the physiological steady state is largely redundant. Instead, its immune-modulatory role plays a significant role in the development or resolution of autoimmune diseases. This is mediated either through the differentiation of precursor cells into specialized non-steady state granulocytes, macrophages and dendritic cells, or through the modulation of the phenotype of mature myeloid cells. Thus, outside of myelopoiesis, GM-CSF has a profound role in regulating the immune response and maintaining immunological tolerance. Copyright © 2015 Elsevier Ltd. All rights reserved.
    No preview · Article · Jun 2015 · Cytokine
Show more

Questions & Answers about this publication