Artificial Intelligence (AI) has become a transformative tool in scientific research, reshaping traditional methodologies by enabling advanced data analysis, hypothesis testing, and predictive modeling. The integration of machine learning (ML), deep learning (DL), and natural language processing (NLP) has significantly accelerated discoveries in medicine, physics, chemistry, environmental science, and other disciplines. AI-driven technologies allow researchers to process large datasets, identify complex patterns, and generate predictive insights with unprecedented accuracy and speed. These innovations have led to breakthroughs in drug discovery, climate modeling, quantum physics simulations, and genetic research, demonstrating AI’s potential to enhance efficiency, automation, and precision in scientific investigations. Despite its numerous advantages, AI-driven research presents challenges, including ethical concerns, algorithmic bias, data security risks, and high computational demands. The reliance on large datasets and complex AI models raises concerns about data privacy, model transparency, and fairness in scientific conclusions. Additionally, AI systems require high-performance computing resources, making accessibility and affordability key concerns for many research institutions. Addressing these challenges through robust regulatory frameworks, ethical AI development, and improved AI model interpretability is crucial for ensuring responsible AI-driven scientific exploration. This study explores AI’s impact on scientific research, analyzing its applications, benefits, and challenges. The findings are supported by statistical data and two tables, illustrating AI’s adoption trends, efficiency improvements, and transformative role in modern research. Future advancements, such as AI-augmented automation, AI-driven robotics, and interdisciplinary AI applications, will further revolutionize scientific inquiry, making AI an indispensable tool for data-driven discovery and innovation.