The Saigon River is the important source for water supply in Ho Chi Minh City. However, its water quality is degrading gradually. This study was conducted in order to assess its pollution status and risks of those pollutants for safety water supply. There were 9 water samples collected from upstream to downstream of Sai Gon River, 10 water samples from canals of Sai Gon River system. Raw water and water after treatment of the Tan Hiep water supply treatment plant, which has exploited Sai Gon river water for water supply in Ho Chi Minh City, were also collected. Monitoring parameters include physical, chemical and microbiological parameters and some selected metals such as As, Cu, Cd, Mn, Fe, Cr, Pb and Zn. The results showed that downstream area of Sai Gon River was more polluted than upstream. Particularly, bacteria, organic matters, Fe, Cd and Mn had higher potential risk and may affect on human health as well as safety water supply. The Hazard Indices of almost metals were less than 1, except Mn and Cd were more than 0.5. Treated water from Tan Hiep water supply treatment plant met the Vietnamese water quality standard for water supply and safety for consumers’ health.