Overactive bladder (OAB) has become an increasing field of interest. Traditional use indicates that pumpkin seed (Cucurbita pepo L.) may be helpful in combating its symptoms. Additionally, soy isoflavones (Glycine max) are well-documented for hormonal imbalance related indications. The present randomized, double-blind, placebo-controlled study evaluated efficacy and safety of Cucuflavone (containing extracts of pumpkin seed and soy germ) in 120 subjects suffering from OAB. After 12 weeks, subjects taking Cucuflavone experienced a significant reduction versus baseline, in (1) urination frequency, (2) urgency, (3) incontinence frequency, maximum urgency score, (5) nocturnal urination frequency and (6) OAB-symptom scale. The placebo group reached significant differences for (1), (3) and (6). No adverse events or abnormal changes in safety parameters occurred. Thus, the combination of pumpkin seed and soy germ extract can be used as natural alternative to relieve symptoms of OAB and to improve quality of life.