Three to seven oriented paleomagnetic samples were collected from 16 sites in the Nelson Bay and Bridgewater formations at Portland, Victoria, which contains the recently discovered Nelson Bay local fauna (L.F.). The entire section has reversed polarity. These results, along with Globorotalia truncatulinoides within the section, and the presence of underlying middle Pliocene-dated basalts, ... [Show full abstract] indicate that the Portland section, and the included Nelson Bay L.F., was deposited within the late Matuyama Chron between 1.66 and 0.73 myr ago. This represents the first well-documented pre-14C Pleistocene mammalian fauna in Australia calibrated in direct stratigraphic context with absolute dating methods. In addition, the reversed polarity for the Bridgewater Formation confirms the previous hypothesis that the depositional history of this beach-sand deposit is time-transgressive across the Brunhes-Matuyama boundary.