Structure determination of noncanonical RNA motifs guided by H NMR chemical shifts

Nature Methods (Impact Factor: 32.07). 03/2014; 11(4). DOI: 10.1038/nmeth.2876
Source: PubMed


Structured noncoding RNAs underlie fundamental cellular processes, but determining their three-dimensional structures remains challenging. We demonstrate that integrating (1)H NMR chemical shift data with Rosetta de novo modeling can be used to consistently determine high-resolution RNA structures. On a benchmark set of 23 noncanonical RNA motifs, including 11 'blind' targets, chemical-shift Rosetta for RNA (CS-Rosetta-RNA) recovered experimental structures with high accuracy (0.6-2.0 Å all-heavy-atom r.m.s. deviation) in 18 cases.

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Available from: Michèle C Erat, Apr 20, 2015
    • "Predictive approaches, in addition to the well-established experimental approaches such as X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, are being developed to quickly and accurately decipher three-dimensional structure. It is likely that a combination of prediction and experiment, such as has been shown for secondary structure prediction, will allow for accurate models of RNA three-dimensional structure to be generated quickly (Sripakdeevong et al., 2014). "
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