Designing and Testing the Activities of TAL Effector Nucleases

Methods in molecular biology (Clifton, N.J.) (Impact Factor: 1.29). 02/2014; 1114:203-19. DOI: 10.1007/978-1-62703-761-7_13
Source: PubMed


Transcription activator-like effector nucleases (TALENs) have rapidly developed into a powerful tool for genome editing. To avoid labor-intensive and time-consuming experimental screening for active TALENs, a scoring system can help select optimal target sites. Here we describe a procedure to design active TALENs using a scoring system named Scoring Algorithm for Predicted TALEN Activity (SAPTA) and a method to test the activity of individual and pairs of TALENs.

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Available from: Thomas James Cradick, Jan 05, 2015
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