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Anti-inflammatory and Analgesic Activities of Artemisia absinthium and Chemical Composition of its Essential Oil

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Nature has been a source of medicinal agents for thousands of years and has been isolated the number of modern drugs from natural resources. Artemisia absinthium used for a variety of medicinal purposes and therapeutic targets in all over the world, such as localized pains, contusion inflammation, anti-rheumatic, include fever reduction, digestive ailments and muscle pain. This study aimed to assess the anti-inflammatory and anti-nociceptive activity of essential oil and aqueous extract from Artemisia absinthium for the first time. Chemical compositions of the essential oil were determined by GC/MS. The anti-inflammatory activity was evaluated by carrageenan-induced paw edema in mice. Analgesic activity was assessed by acetic acid-induced writhing, formalin and hot plate tests in mice. Pretreatment with the essential oil (at the dose of 2, 4 and 8mg/kg) and aqueous extract (50, 100 and 200mg/kg) showed potential anti-inflammatory and anti-nociceptive effects to different level. The essential oil at 4 and 8 mg/kg significantly reduced carrageenan induced paw edema. The essential oil and aqueous extract produced significant decreased number of writhing in acetic acid-induced writhing model and increased the response latency in hot plate test after 30 min. Both Essential oil and aqueous extract significantly suppressed in a dose-dependent manner the nociceptive response in the formalin test, while the effect on the late phase was more pronounced. GC–MS analyses showed the presence of twenty components in essential oil. The essential oil and aqueous extract possesses excellent anti-inflammatory activity as well as antinociceptive properties especially peripheral analgesic. Keywords: Artemisia absinthium, essential oil, aqueous extract, anti-inflammation, analgesic, GC/MS.
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