Article

Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat

Frontiers in Plant Science (Impact Factor: 3.95). 01/2014; 4:520. DOI: 10.3389/fpls.2013.00520
Source: PubMed

ABSTRACT

Wheat leaf rust, caused by the basidiomycete Puccinia triticina, can cause yield losses of up to 20% in wheat producing regions. During infection, the fungus forms haustoria that secrete proteins into the plant cell and effect changes in plant transcription, metabolism, and defense. It is hypothesized that new races emerge as a result of overcoming plant resistance via changes in the secreted effector proteins. To understand gene expression during infection and find genetic differences associated with races, RNA from wheat leaves infected with six different rust races, at 6 days post inoculation, was sequenced using Illumina. As P. triticina is an obligate biotroph, RNA from both the host and fungi were present and separated by alignment to the P. triticina genome and a wheat EST reference. A total of 222,571 rust contigs were assembled from 165 million reads. An examination of the resulting contigs revealed 532 predicted secreted proteins among the transcripts. Of these, 456 were found in all races. Fifteen genes were found with amino acid changes, corresponding to putative avirulence effectors potentially recognized by 11 different leaf rust resistance (Lr) genes. Twelve of the potential avirulence effectors have no homology to known genes. One gene had significant similarity to cerato-platanin, a known fungal elicitor, and another showed similarity to fungal tyrosinase, an enzyme involved in melanin synthesis. Temporal expression profiles were developed for these genes by qRT-PCR and show that the genes expression patterns were consistent between races from infection initiation to just prior to spore eruption.

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Available from: David L Joly, Jul 24, 2014
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    • "tritici, the flax rust Melampsora lini and the coffee rust Hemileia vastatrix (Cantu et al., 2011, 2013; Duplessis et al., 2011a; Zheng et al., 2013; Cristancho et al., 2014; Nemri et al., 2014). Secretomes of rust fungi have been determined based on the presence of predicted N-terminal signal peptides in proteins (Cantu et al., 2011, 2013; Duplessis et al., 2011a; Fernandez et al., 2012; Hacquard et al., 2012; Saunders et al., 2012; Bruce et al., 2013; Garnica et al., 2013; Zheng et al., 2013; Link et al., 2014; Nemri et al., 2014). Signal peptides can be defined using predictors available online (Emanuelsson et al., 2007). "
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