Effect of probiotic Ecobiol on broiler performance.
, Yañez P.
, Gracia M.
, Mallo J.J.
NOREL S.A., Madrid, Spain
Huevos León, León, Spain
Imasde Agroalimentaria S.L., Madrid, Spain
firstname.lastname@example.org, email@example.com, firstname.lastname@example.org, email@example.com
The objective of the present study was to determine if the addition of Bacillus
Amyloliquefaciens spores (Ecobiol) to the diet could have a beneficial effect on broilers
performance. The study was conducted in the commercial farm “Huevos León” sited in León
According to literature, probiotics can improve performance by different modes of
action as: digestive enzymes excretion, lactic acid production (KAUPP, 1925), positive effect
on immune system effect (CORTHESY, 2007; HERICH, 2002; KLASING, 2007; LJUNGH,
2006; SIRAGUSA, 2012; JERZSELE, 2011), improve vitality (MALLO, 2010). In general,
probiotics can improve conversion, decrease mortality, stimulate the immune response and
protect against enteric pathogens (SIRAGUSA, 2012)
Bacillus amyloliquefaciens CECT 5940 (the microorganism present in Ecobiol) is one
of the strains approved in UE and registered with number 4B1822 as enhancer in broiler
MATERIAL AND METHODS
Animals were housed in clean and disinfected buildings equipped with automatic
feeders and drinkers. Light schedule and temperature were the recommended by the integrator
and were daily monitored. Water was available in a sufficient quantity for the number of
animal and feed was administered ad libitum.
10 different buildings will be used.
o 2 buildings with 15,000 birds each. Buildings 1-2
o 2 buildings with 14,000 birds each. Buildings 3-4
o 3 buildings with 30,000 birds each. Buildings 5-7
o 3 buildings with 21,000 birds each. Buildings 8-10
A total of 211,000 animals were allocated at random to 2 different treatments:
T1: Control Diet
T2: Control Diet + 1 kg/Tm ECOBIOL
CFU/gr of feed).
The following feeding Schedule was used:
Treatment 0-12 days 12-22 days 22-38 days 38-45 days
T-1 Starter Growing Fattening Finisher
The experimental unit was the building.
The addition of any other growth promoter was avoided not to bias the result of the
The following parameters were measured
- FBW: average weight (per building) sampled in all periods (12 days, 22 days, 38
days and 45 days)
- ADFI: (per building) in all periods.
- ADG and FCR were calculated according to the data above mentioned. These
parameters will be calculated for each period and for the global period (0-45 days).
- Daily monitored per building
- Abnormal situations and comments were also recorded
Raw data was sent to Imasde Agroalimentaria S.L for the statistical analysis. A
completely randomized design by GLM of SAS was used to analyze the data.
Mortality % Days to abattoir FBW FCR EPEF*
T-1 6.77 49.45 2,644 2.06 242
T-2 6.28 46.97 2,575 1.98 259
EEM (n=4) 3.32 0.61 71.39 0.03
P 0.9206 0.0351 0.5261 0.0906
It can be concluded that broiler receiving Ecobiol at 1 kg/mT had a better performance
than control group. It is remarkable that an equal final weigh was obtained with nearly 2.5
days less in the Ecobiol group. Also the feed conversion tended to be better. The addition of
the two effects resulted in a better European efficiency factor.
CORTHÉSY B., H. R. GASKINS, A. MERCENIER. 2007. Cross-Talk between Probiotic
Bacteria and the Host Immune System. Journal of Nutrition. 137: 781S-790S
HERICH R., M. LEVKUT. 2002. Lactic acid bacteria probiotics and immune system.
Veterinary Medicine-Czech. 47:169-180
JERZSELE Á., K. SZEKÉR, P. GÁLFI, M. PUYALTO, P. HONRUBIA, J. J. MALLO.
2011. Effects of protected sodium-n-butyrate (BP70), its combination with essential oils
(BP70+EO) and of a B. amyloliquefaciens probiotic (Ecobiol) in a necrotic enteritis artificial
infection model in broilers. 2011 international Poultry Scientific Forum proceedings. Abs. 1
KAUPP B. F. AD R. S. DEARSTYNE. 1925. The effects of lactic acid on B. pullorum, B.
avisepticus and B. sanguinarium and its possible role in the control of intestinal diseases of
poultry. Poultry Science. 4: 242-249
Klasing K. C. 2007. Nutrition and the immune system. British Poultry Science. 48: 525-537
LJUNGH A. AND T. WADSTRÖM. 2006. Lactic acid bacteria as Probiotics. Current Issues
Intestinal Microbiology. 7: 73-90
MALLO J. J., M. I. GRACIA, P. HONRUBIA, G. SEDANO. 2010. Use of a Bacillus
amyloliquefaciens probiotic in broiler farms. Poultry Science Association annual meeting.
SIRAGUSA G. R. 2012. Modern probiology- Direct fed microbials and the avian gut
microbiota. Proceedings of the Australian Poultry Science Symposium. 23. 120:133