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Effect of probiotic Ecobiol on broiler performance.

  • Imasde Agroalimentaria, S.L.


To determine the effect that a Bacillus amyloliquefaciens probiotic has on broilers performance a total of 211,000 animals were allocated at random to 2 different treatments (T1: Control diet; T2 Control diet + 1kg/Tm ECOBIOL 10^6 CFU/gr of feed). Birds were housed in 10 different buildings (5 buildings per treatment). Feeds and water were offered ad libitum. The experimental unit was the building, data were corrected for farm effect. BW, ADG, ADFI and FCR were recorded for the whole fattening period. Data were analyzed as a completely randomized design by GLM of SAS. Birds fed T2 tended to have better FCR (1.98 vs. 2.06; P<0.09) than T1. There was also a significant difference in number of days to slaughter weight, (T1=49.45; T2= 46.97; P<0.035). European efficiency factor (EPEF) was 7.24% higher in Ecobiol group. It was concluded that T1 improved animal’s performance.
Effect of probiotic Ecobiol on broiler performance.
Ortiz A.
, Yañez P.
, Gracia M.
, Mallo J.J.
NOREL S.A., Madrid, Spain
Huevos León, León, Spain
Imasde Agroalimentaria S.L., Madrid, Spain,,,
The objective of the present study was to determine if the addition of Bacillus
Amyloliquefaciens spores (Ecobiol) to the diet could have a beneficial effect on broilers
performance. The study was conducted in the commercial farm “Huevos León” sited in León
According to literature, probiotics can improve performance by different modes of
action as: digestive enzymes excretion, lactic acid production (KAUPP, 1925), positive effect
on immune system effect (CORTHESY, 2007; HERICH, 2002; KLASING, 2007; LJUNGH,
2006; SIRAGUSA, 2012; JERZSELE, 2011), improve vitality (MALLO, 2010). In general,
probiotics can improve conversion, decrease mortality, stimulate the immune response and
protect against enteric pathogens (SIRAGUSA, 2012)
Bacillus amyloliquefaciens CECT 5940 (the microorganism present in Ecobiol) is one
of the strains approved in UE and registered with number 4B1822 as enhancer in broiler
Animals were housed in clean and disinfected buildings equipped with automatic
feeders and drinkers. Light schedule and temperature were the recommended by the integrator
and were daily monitored. Water was available in a sufficient quantity for the number of
animal and feed was administered ad libitum.
10 different buildings will be used.
Farm: Alejandro
o 2 buildings with 15,000 birds each. Buildings 1-2
o 2 buildings with 14,000 birds each. Buildings 3-4
Farm: Montejos
o 3 buildings with 30,000 birds each. Buildings 5-7
o 3 buildings with 21,000 birds each. Buildings 8-10
A total of 211,000 animals were allocated at random to 2 different treatments:
T1: Control Diet
T2: Control Diet + 1 kg/Tm ECOBIOL
CFU/gr of feed).
The following feeding Schedule was used:
Treatment 0-12 days 12-22 days 22-38 days 38-45 days
T-1 Starter Growing Fattening Finisher
1 kg/tn
1 kg/tn
1 kg/tn
1 kg/tn
The experimental unit was the building.
The addition of any other growth promoter was avoided not to bias the result of the
The following parameters were measured
Performance parameters
- FBW: average weight (per building) sampled in all periods (12 days, 22 days, 38
days and 45 days)
- ADFI: (per building) in all periods.
- ADG and FCR were calculated according to the data above mentioned. These
parameters will be calculated for each period and for the global period (0-45 days).
- Daily monitored per building
- Abnormal situations and comments were also recorded
Raw data was sent to Imasde Agroalimentaria S.L for the statistical analysis. A
completely randomized design by GLM of SAS was used to analyze the data.
Mortality % Days to abattoir FBW FCR EPEF*
T-1 6.77 49.45 2,644 2.06 242
T-2 6.28 46.97 2,575 1.98 259
EEM (n=4) 3.32 0.61 71.39 0.03
P 0.9206 0.0351 0.5261 0.0906
It can be concluded that broiler receiving Ecobiol at 1 kg/mT had a better performance
than control group. It is remarkable that an equal final weigh was obtained with nearly 2.5
days less in the Ecobiol group. Also the feed conversion tended to be better. The addition of
the two effects resulted in a better European efficiency factor.
CORTHÉSY B., H. R. GASKINS, A. MERCENIER. 2007. Cross-Talk between Probiotic
Bacteria and the Host Immune System. Journal of Nutrition. 137: 781S-790S
HERICH R., M. LEVKUT. 2002. Lactic acid bacteria probiotics and immune system.
Veterinary Medicine-Czech. 47:169-180
2011. Effects of protected sodium-n-butyrate (BP70), its combination with essential oils
(BP70+EO) and of a B. amyloliquefaciens probiotic (Ecobiol) in a necrotic enteritis artificial
infection model in broilers. 2011 international Poultry Scientific Forum proceedings. Abs. 1
KAUPP B. F. AD R. S. DEARSTYNE. 1925. The effects of lactic acid on B. pullorum, B.
avisepticus and B. sanguinarium and its possible role in the control of intestinal diseases of
poultry. Poultry Science. 4: 242-249
Klasing K. C. 2007. Nutrition and the immune system. British Poultry Science. 48: 525-537
LJUNGH A. AND T. WADSTRÖM. 2006. Lactic acid bacteria as Probiotics. Current Issues
Intestinal Microbiology. 7: 73-90
MALLO J. J., M. I. GRACIA, P. HONRUBIA, G. SEDANO. 2010. Use of a Bacillus
amyloliquefaciens probiotic in broiler farms. Poultry Science Association annual meeting.
Abs. 978
SIRAGUSA G. R. 2012. Modern probiology- Direct fed microbials and the avian gut
microbiota. Proceedings of the Australian Poultry Science Symposium. 23. 120:133
... Laboratory analyses did not reveal gastrointestinal parasites, Escherichia coli, Eimeria sp. and Salmonella sp. in the examined bird (González et al. 2016). Ortiz [2013], analyzing the effect of the probiotic ECOBIOL with the inclusion of Bacillus amyloliquefaciens CECT 5940, reported that the final weight of 2575 g was obtained 2.5 days earlier than in the control group. Feed conversion was 1.98 vs. 2.06, and mortality reduction was 6.28% vs. 6.77%. ...
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Probiotics are feed additives that have gained popularity in poultry. They are one of the more universal feed additives and can be easily combined with other additives. This study was designed to evaluate the impact of including recommended doses of probiotics and growth-promoting antibiotics in concentrated foods on productivity and gut health in broilers. Six hundred one-day-old unsexed broiler chicks (Ross 308 strain) were used in this study. They were divided into three groups (40 chicks per group). Group T0 received balanced feed with growth stimulating antibiotic (Zinc Bacitracin), while group T1 received balanced diet (no growth stimulating antibiotic) + probiotic (Bacillus subtilis sp.; 1.6 × 10 9 CFU g-1) at an inclusion rate of 500 g ton-1. Group T 2 was fed the basal balanced diet with a growth-stimulating antibiotic (Zinc Bacitracin) and a probiotic (Bacillus subtilis sp.). Statistical analysis and processing the material were performed using the data analysis package "MS Excel 2010" and the program "Statistics for Windows". In this study, we assessed the effect of supplementation of probiotics and acidifiers as well as their combination on broiler live body weight, net carcass, along with dressing percentage, the weight of the internal organs, relative bowel length (small intestine length, large intestine length, caecum length, and fabric bag length) and weight of broiler cuts breast. The outcomes revealed a difference between the control and other treatment groups which was statistically significant (p < 0.05). The ratio of villus height to crypt depth and villus height in the duodenum and ileum were both raised (p < 0.05) by the addition of either probiotics or synbiotics.
... So far, many kinds of Bacillus spp. have been authorized for utilization in broiler diets as direct-fed microbials, such as Bacillus subtilis, Bacillus coagulans, Bacillus licheniformis, and Bacillus amyloliquefaciens (Ortiz et al., 2013;Elshaghabee et al., 2017). ...
Full-text available
This experiment was conducted to investigate the effects of dietary supplementation with Bacillus amyloliquefaciens CECT 5940 (BA-5940) on growth performance, antioxidant capacity, immunity, and digestive enzyme activity of broiler chickens. A total of 720 one-day-old Arbor Acres male broiler chicks (average body weight, 45.87 ± 0.86 g) were randomly allocated to 5 treatments of 8 replicates with 18 chicks in each replicate. Broilers in the control group were fed a corn-wheat-soybean basal diet, the other 4 groups were fed the same basal diet supplemented with 500, 1,000, 1,500, or 2,000 mg/kg Ecobiol® (1.27 × 10⁹ CFU/g BA-5940) for 42 days, respectively. Broilers fed diets supplemented with BA- 5940 showed a quadratic response (P< 0.05) of average daily gain (ADG) and feed to gain ratio (F:G) during d 22-42 and d 0-42. The glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities in serum and liver, and T-AOC in liver of broilers on d 42 increased linearly (P < 0.05) with increasing levels of BA-5940, while MDA level in serum decreased linearly (P <0.05). Concentrations of serum immunoglobulin (Ig) A and IgM on d 21, and IgM on d 42 increased linearly (P < 0.05) as BA-5940 levels increased. Supplementation with increasing levels of BA-5940 linearly decreased serum tumor necrosis factor-α (TNF-α) levels on d 21 and 42, while increased interleukin (IL)-10 concentration (linear, P < 0.05) on d 21. Meanwhile, the levels of IL-1β, IL-6, and TNF-α in the mucosa of jejunum and ileum were decreased (linear, P < 0.05) on day 42 as dietary supplementation of BA-5940 increased. Additionally, supplementation with BA-5940 also increased the activities of amylase (linear, P < 0.01), lipase (linear, P < 0.05) and chymotrypsin (linear, P < 0.01) in jejunal digesta, and lipase (linear, P < 0.05) in ileal digesta of broilers on d 42. To summarize, inclusion of BA-5940 in corn-wheat-soybean meal-based diet improved growth performance of broilers through improving antioxidant capacity, immunity, and digestive enzyme activity. Based on the results of this study, 1.1-1.6 × 10⁹ CFU/kg BA-5940 is recommended for supplementation in broiler diets.
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Aims: Sub-therapeutic use of antibiotics as a growth promoter in animal diets has either been banned or voluntarily withdrawn from use in many countries to help curb the emergence of antibiotic-resistant pathogens. Probiotics may be an alternative to antibiotics as a growth promoter. We investigated the effects of a novel probiotic strain Bacillus amyloliquefaciens H57 (H57) on the performance and microbiome-associated metabolic potential. Methods and results: Broiler chickens were fed either sorghum- or wheat-based diets supplemented with the probiotic H57. The growth rate, feed intake and feed conversion in supplemented birds were compared with those in non-supplemented control. Caecal microbial metabolic functions were studied with shotgun metagenomic sequencing. H57 supplementation significantly increased the growth rate and daily feed intake of meat chickens relative to the non-supplemented controls without any effect on feed conversion ratio. In addition, relative to the non-supplemented controls, gene-centric metagenomics revealed that H57 significantly altered the functional capacity of the caecal microbiome, with amino acid and vitamin synthesis pathways being positively associated with H57 supplementation. Conclusions: Bacillus amyloliquefaciens H57 improves the performance of meat chickens or broilers and significantly modifies the functional potential of their caecal microbiomes, with enhanced potential capacity for amino acid and vitamin biosynthesis.A feed supplement like H57, with the ability to enhance weight gain through modulation of intestinal microbial functions, has the potential to be an alternative to antibiotic growth promoters and provide substantial benefits to the poultry industry.
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In order to evaluate the effect of three zootechnical additives of Bacillus subtilis on productive and health indicators in broilers, a study was conducted in the poultry farm of the Institute of Animal Science Mayabeque, Cuba. For such purpose 200 broilers of the Cuban breeding hybrid EB34 were used, in a completely randomized design. Four treatments were studied: T1: control group (CG), T2: C-31, T3: C-34 and T4: E-44, at a dose of 10(9) endospores per gram of concentrate feed. The trial lasted 42 days, and hematological, immunological, productive and health indicators were evaluated. The use of these zootechnical additives did not cause any effect at 21 days on any of the indicators; however, at 35 and 42 days the weight of the bursa of Fabricius, the spleen and the hemagglutination titers to Newcastle vaccine increased (p
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Mucous membranes of the body are in direct contact with the outside environment and they are colonised by a large number of different bacteria. rough mucous membranes, the organism is in permanent con - tact with different antigens. Mucous surfaces are protected by many defence mechanisms that ensure a permanent and effective protection. ey include the production of secretory IgA, the production of mucus, cytoprotective peptides, defensins etc. Indigenous microflora markedly affects the structure of the host mucous, its function, and the development of the whole immune system. Protective microflora prevents pathogens from adhering by competi - tion for substrates and places of adhesion, and they simultaneously produce antibacterial substances and stimulate the production of specific antibodies and mucus. e early colonisation of the gut with living micro-organisms is important for the development of the gut protection barrier. e number of immune and epithelial cells increases. Probiotic micro-organisms including lactic acid bacteria (LAB) positively influence the composition of the gut microflora; they stimulate the production of secretory IgA; they affect the targeted transportation of the luminal antigens to Peyer's patches and they increase the production of IFN-γ. LAB stimulate the activity of non-specific and specific immune cells. ese properties of the LAB depend on the particular species or strain of bacteria. ese singularities are probably determined by differences in the cell wall composition. LAB belong to a group of benefi - cially acting bacteria and they are able to eliminate damage to the gut microenvironment; they stimulate local and systemic immune responses and they maintain the integrity of the gut wall.
A number of Lactobacillus species, Bifidobacterium sp, Saccharomyces boulardii, and some other microbes have been proposed as and are used as probiotic strains, i.e. live microorganisms as food supplement in order to benefit health. The health claims range from rather vague as regulation of bowel activity and increasing of well-being to more specific, such as exerting antagonistic effect on the gastroenteric pathogens Clostridium difficile, Campylobacter jejuni, Helicobacter pylori and rotavirus, neutralising food mutagens produced in colon, shifting the immune response towards a Th2 response, and thereby alleviating allergic reactions, and lowering serum cholesterol (Tannock, 2002). Unfortunately, most publications are case reports, uncontrolled studies in humans, or reports of animal or in vitro studies. Whether or not the probiotic strains employed shall be of human origin is a matter of debate but this is not a matter of concern, as long as the strains can be shown to survive the transport in the human gastrointestinal (GI) tract and to colonise the human large intestine. This includes survival in the stressful environment of the stomach - acidic pH and bile - with induction of new genes encoding a number of stress proteins. Since the availability of antioxidants decreases rostrally in the GI tract production of antioxidants by colonic bacteria provides a beneficial effect in scavenging free radicals. LAB strains commonly produce antimicrobial substance(s) with activity against the homologous strain, but LAB strains also often produce microbicidal substances with effect against gastric and intestinal pathogens and other microbes, or compete for cell surface and mucin binding sites. This could be the mechanism behind reports that some probiotic strains inhibit or decrease translocation of bacteria from the gut to the liver. A protective effect against cancer development can be ascribed to binding of mutagens by intestinal bacteria, reduction of the enzymes beta-glucuronidase and beta-glucosidase, and deconjugation of bile acids, or merely by enhancing the immune system of the host. The latter has attracted considerable interest, and LAB have been tested in several clinical trials in allergic diseases. Characteristics ascribed to a probiotic strain are in general strain specific, and individual strains have to be tested for each property. Survival of strains during production, packing and storage of a viable cell mass has to be tested and declared.
Among the numerous purported health benefits attributed to probiotic bacteria, their capacity to interact with the immune system of the host is now supported by an increasing number of in vitro and in vivo experiments. In addition to these, a few well-controlled human intervention trials aimed at preventing chronic immune dysregulation have been reported. Even though the precise molecular mechanisms governing the cross-talk between these beneficial bacteria and the intestinal ecosystem remain to be discovered, a new and fascinating phase of research has been initiated in this area as demonstrated by a series of recent articles. This article summarizes the status and latest progress of the field in selected areas and aims at identifying key questions that remain to be addressed, especially concerning the translocation of ingested bacteria, the identification of major immunomodulatory compounds of probiotics, and specific aspects of the host-microbe cross-talk. The interaction with immunocompetent cells and the role of secretory IgA in gut homeostasis are also evoked. Finally, a brief overview is provided on the potential use of recombinant DNA technology to enhance the health benefits of probiotic strains and to unravel specific mechanisms of the host-microbe interaction.
1. Infectious diseases reduce productivity and diminish animal welfare. 2. Appropriate nutrition may aid in minimising the incidence of diseases by enhancing immunity. 3. An understanding of the pressures imposed by evolution that underlie poultry nutrition as well as those which underlie immunity provides focus to the field of nutritional immunology. Additional understanding is provided by knowing the specific cellular mechanisms by which diet affects immunity, and how these mechanisms pertain to specific nutrients and pathogens. 4. These approaches indicate that higher inclusion rates of all nutrients are not always better and that the traditional ideas of adding a surfeit of specific nutrients to improve immunity does not usually serve us well. 5. Understanding the nuances of nutrition and immunity is important for optimising bird health and productivity, and will be an important contributor towards fulfilling the consumer's conflicting demands for more natural production and better animal welfare.
The effects of lactic acid on B. pullorum, B. avisepticus and B. sanguinarium and its possible role in the control of intestinal diseases of poultry
  • F Kaupp B
  • S Ad R
  • Dearstyne
KAUPP B. F. AD R. S. DEARSTYNE. 1925. The effects of lactic acid on B. pullorum, B. avisepticus and B. sanguinarium and its possible role in the control of intestinal diseases of poultry. Poultry Science. 4: 242-249
Modern probiology-Direct fed microbials and the avian gut microbiota
  • G R Siragusa
SIRAGUSA G. R. 2012. Modern probiology-Direct fed microbials and the avian gut microbiota. Proceedings of the Australian Poultry Science Symposium. 23. 120:133