In micropropagation of ash morphology of expiants depended of the nature of the gelling agent. With agar they appeared vigoumus whereas with Technogel®they were vitreous. In other respects, when transferred onto an agar solidified medium, the abnormal plantlets formed normal new organs. With this system of culture, considered as a vitrification inducing system, the involvement of ethylene was ... [Show full abstract] studied.
Technoge1® production of ethylene. Moreover, AgNO3 without affecting ethylene release succeeded in a decrease of vitrification. Lastly, CoC12 greatly reduced both ethylene synthesis and vitrification.
On agar medium, ACC or ethephon increased ethylene concentration in culture vessels but never induced vitrification.
Differences in physiological states induced by using different gelling agents, could lead to differences in sensitivity to ethylene and thus to formation either of normal or vitreous expiants.