Leptospirosis, a zoonotic disease, is caused by pathogenic spirochetes belonging to the genus Leptospira. It is widely considered one of the most prevalent zoonoses in terms of both geographic distribution and the variety of animal species susceptible to acute illness or acting as renal carriers. Tropical and subtropical regions, such as Thailand, experience a higher incidence of leptospirosis in humans and animals, making it a significant public health concern in those areas.While clinical signs of leptospirosis in cats have yet to be thoroughly investigated, previous studies have shown that cats with polyuria and polydipsia are more likely to have anti-Leptospira antibodies. However, in cats, the clinical signs are usually mild, despite the presence of leptospires in their blood and urine. Reported clinical signs in infected cats (confirmed through MAT and/or PCR) include polyuria, polydipsia, haematuria, uveitis, lameness, lethargy, anorexia, weight loss, ascites, vomiting, diarrhea, pain on handling, and inflammatory lesions on the skin and digits. Various diagnostic tools can be employed, such as the microscopic agglutination test (MAT), indirect hemagglutination assay (IHA), or immuno-enzymatic assays (ELISA) to detect specific antibodies. Leptospira or their components can also be identified in urine or tissues through culture, dark field microscopy, immunostaining, or PCR.Human infections of leptospirosis can be acquired by individuals in certain occupations, such as veterinarians, farmers, animal caretakers, and researchers, as well as people exposed to pet dogs or domestic livestock during their daily activities. Farmers, veterinarians, and abattoir workers are particularly at risk due to their occupation.