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Vaginal cytology as a method of estrous determination in the female agouti ( Dasyprocta leporina)

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Vaginal cytology as a method of estrous determination in the female agouti (Dasyprocta leporina) - Volume 1 Issue 2 - Michele Singh, Gary W. Garcia, Andrew O. Adogwa, Gregory Bourne
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towards eating smaller kid goat meat has developed in many counties but more so in the tropical regions of the Indian subcontinent which
includes Pakistan, India, Bangladesh, Nepal, Sri Lanka etc. An average carcass weight of 10–14 kg for the goat is considered normal but the
tender meat of goats of dressed carcass weight as low as 3–4 kg has emerged for certain tastes. It is achieved by early slaughtering of teddy
breeds of goats in India and Pakistan. Sometimes these small goats look like big cats roaming the slaughterhouse floors in these countries.
Quality traits of any kind of red meat can be divided into three broad lines, composition of the meat to indicate nutritive value, physical
characteristics for visual acceptance and processing ease as it relates to the biochemical parameters indicative of normal processing
treatments. Goat meat has brick red color and chalk white fat; both colors darken with the age of the animal. The processing characteristics of
chevon
are known to be as good as of other meats, so it can be easily put in place of beef or beefalo. In some respects like its use in emulsion
products, it is as good as lamb and better than beef.
From the nutritional point of view goat meat is a very important source of animal protein in most developing countries, however, not enough
research seems to have been carried out on its different nutritional properties and aspects. An average composition of goat meat is as follows,
moisture 74–76%, protein 20.6–22.3%, fat 0.5–2.5% and ash about 1.0%. Chevon contains more essential amino acids like arginine, leucine
and iso-leucine than sheep meat. Goat meat has a lesser fat content and appears to have more oleic acid in its fat depotsthan sheep. Goat meat
has higher content of thiamine and riboflavin in the liver but it is low in niacin. Management factors and practices affect goat meat yield and
meat quality, like tenderness and juiciness. The paper reviews most of the related aspects of goat meat to conclude some important points.
doi:10.1017/S2040470010000440
Vaginal cytology as a method of estrous determination in the
female agouti (
Dasyprocta leporina
)
Michele Singh
1
-
, Gary W. Garcia
1
, Andrew O. Adogwa
2
and Gregory Bourne
2
1
The Open Tropical Forage-Animal Production Laboratory (OTF-APL), Department of Food Production (DFP), Faculty of Science and Agriculture, The University of the
West Indies (UWI), St. Augustine, Trinidad and Tobago, West Indies;
2
School of Veterinary Medicine, Faculty of Medical Sciences, UWI, St. Augustine, Trinidad and
Tobago, West Indies
Introduction
Intense devastation of tropical ecosystems has been observed in recent times, with irretrievable loss of neo tropical animal genetic resources.
This degradation of tropical forests and wildlife has led to the need for innovations with regards to captive rearing of neo tropical animal
species, in an attempt to conserve and produce animal protein for local consumption. The agouti (
Dasyprocta leprorina
) is a neo tropical
rodent, found in Central and South America and the Caribbean. Current research suggests that the agouti possess tremendous potential for
domestication and semi-commercial production.
.
Knowledge of reproductive physiology forms an integral part of captive breeding strategies.
The manipulation of the reproductive cycle of the female agouti is a method of increasing the animal’s fecundity. Vaginal cytology is a simple
technique used to help characterize stages of the reproductive cycle of females and to evaluate certain diseases of the genital tract. Vaginal
cytology is usually used in conjunction with the physical examination, clinical history, vaginoscopy, and hormonal assays to determine the
stage of the reproductive cycle. This is especially important if artificial insemination is to be performed. The estrous cycle of the female agouti
contains four stages that can be followed cytological. These stages are proestrus, estrus, diestrus, and metestrus and each stage is
characterized by the presence, abscence and ratio of typical cell types.
Table 1
General chemical composition of goat meat from Asia.
Components India/Pakistan Malaysia Philippines
Moisture % 74.2 74.0 76.0
Proteins (g 100 g
21
) 21.4 20.6 22.3
Fat (g 100 g
21
) 2.6 2.2 0.6
Ash (g 100 g
21
) 1.1 1.0 1.1
Calcium(Ca) (mg 100 g
21
)12 11 6
Phosphorus(P) (mg 100 g
21
) 193 154 150
Iron (Fe) (mg 100 g
21
) 2.1 0.4
-
E-mail: michele.singh@gmail.com
Advances in Animal Biosciences
417
Materials and Methods
Vaginal smears of five pluriparous females at least two months post partum were observed over a 90 day period. Approximately 5 mL of 10%
saline solution was aspirated 4 cm deep into the cranial vagina and washed 4 times. One milliliter of the final wash was plated on a slide and
observed under the microscope (at 4x mag). Leucocytes, nucleated, enucleated and cornified cells were identified in the swabs, in varying
ratios, coinciding with the four stages of the estrous cycle.
Results and Conclusion
The estrous cycle (mean 6SD) was found to be 31 (64 days) with day 17(62 days) being estrus. It was concluded that vaginal cytology is an
effective method of characterizing the estrous cycle of the female agouti and confirms the findings of Guimara˜es (2000).
Reference
Guimara˜ es DA 2000. Aspectos reprodutivos e endo
´crinos da puberdade, ciclo estral, gestac¸a˜ o e cio po
´s-parto de cutias (Rodentia:Dasyproctidae), criadas em cativeiro.
PhD Thesis, Universidade Federal do Para
´. Bele
´m, Para
´, Brazil. 94 p.
doi:10.1017/S2040470010000452
Dietary electrolyte balance responses in broilers reared in
moderately high environmental temperature and high humidity
Tanveer Ahmad
1
-
, M. Sarwar
2
, Mahr-Un-Nisa
2
, Ahsan-Ul-Haq
3
, J. Ansari
4
and Farooq Iqbal
1
1
Department of Livestock Production and Management, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University, 46300, Rawalpindi, Pakistan;
2
Institute of Animal Nutrition and Feed Technology;
3
Department of Poultry Sciences, University of Agriculture, Faisalabad, Pakistan,
c
Department of Poultry
Husbandry, University of Agriculture, Faisalabad, Pakistan;
4
Poultry Research Institute, Poultry Farms, Bahawalpur, Pakistan
Introduction
High environmental temperature coupled with high humidity, a typical feature of monsoon season, has even more harmful effects on
the performance of broilers since they feel difficulty in dissipating heat through panting. Several management techniques and dietary
modifications have been employed to alleviate heat stress and improve broiler productivity. However, none of these have been considered
as a perfect solution in itself. Borges
et al
. (2003) and Ahmad
et al
. (2005) employed the concept of electrolyte balance (EB) to counter
heat stress in broilers. It was hypothesized that dietary uptake and proper balance of sodium, potassium and chloride can also be helpful
to adjust disturbed blood acid-base balance in broilers reared during the hot and humid season. The present study was designed to determine
an optimal and practical dietary EB in terms of growth performance and physiological adjustment of broilers reared during the hot and
humid season.
Material and methods
A total of 216 broilers were reared up to 42 d in moderately high temperature and humidity. Chicks were exposed to daily cyclic heat stress
(Min. 25.6 and Max. 34.38C, RH 76–78%) after 2 wk of age. The DEB treatments supplying 0, 50, 150, 250 and 350 mEq (Na
1
1K
1
-Cl
2
)kg
21
,
were prepared by adding NaHCO
3
and/or NH
4
Cl to the basal diet without altering K contents (0.71 and 0.65% in starter and finisher diets,
respectively). Basal starter (185 mEq kg
21
) and finisher (172 mEq kg
21
) diets containing NaCl were served as the control. Each diet was
randomly offered to three experimental units having 12 chicks each. The data on live bird performance, water temperature, pH and consumption,
rectal temperature, litter moisture, mortality, carcass characteristics, and blood analysis was collected.
Results
The DEB 50 and 250 mEq kg
21
significantly (
P
,0.05) improved the body weight gain, feed intake, feed:gain ratio (Table 1) and water intake.
Mortality was not influenced significantly (
P
.0.05) by DEB treatments. DEB 50 and 250 significantly lowered the blood alkalotic pH and
HCO3 concentrations as well as maintained the blood base excess values near to zero on the 43rd day. Significantly (
P
,0.01) reduced
heterophils (H), increased lymphocyte (L) percent, H/L ratios, hemoglobin and hematocrit concentration were also noted by DEB 50 and
250 mEq kg
21
. High blood K, Na and Cl were noticed in DEB 250, 350 and 0 mEq kg
21
, respectively. However significantly high blood EB was
noted in DEB 250 mEq kg
21
.
-
E-mail: tanvirah@yahoo.com
Advances in Animal Biosciences
418
... The ovaries were found to be covered by simple, cuboidal to squamous epithelium, with the cortex consisting of cellular connective tissue, in which ovarian follicles-at different stages of maturation-and corpora lutea could be found, while the medulla was made up of loose connective tissue that contained blood vessels [14,[40][41][42]46]. De Oliveira et al. [42] noted the presence of developing follicles and corpus luteum in regression during the proestrus phase; Graafian follicles were present during estrus; a hemorrhagic corpus was present during metestrus; and a mature corpus luteum was present during diestrus. ...
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Full-text available
Simple Summary This literature review focused on the reproductive technologies used in female neo-tropical hystricomorphic rodents. Reproductive technologies aid in efficient reproduction, which is important in these species as they are hunted and valued for their meat. Knowledge of the anatomy and physiology would aid in assisted reproductive techniques, thus attention was given to these areas. Within this group of rodent species there were similar characteristics, some of which have been highlighted as well as any unique features. Some reproductive technologies used included colpocytology, ultrasonography, and hormonal analysis. Abstract This is the second of two literature reviews that focuses on the female reproductive anatomy and reproductive technologies used in selected neo-tropical hystricomorphic rodents. The rodents chosen included the agouti (Dasyprocta leporina), the capybara (Hydrochoerus hydrochaeris), and the paca (Cuniculus paca). Over seventy references were used, spanning the years 1919–2021. Knowledge of the reproductive tract is important in understanding any unique features, which may affect the use of reproductive technologies. Some unique characteristics common to these species included the presence of a vaginal closure membrane and a lobulated placenta with a vascular sub-placenta. The capybara had hyperpigmentation of the vagina that was unique to each individual, while the agouti and paca had accessory corpora lutea, in addition to the main one. Some reproductive technologies have been used, with attempts at estrous induction and synchronization taking place within the past five years. Even though most work has been done over the past twenty years, there is still a dearth of information.
... days (25 to 40 days) with an estrus period of 24 hours. Singh, et al. [9] reported an oestrus cycle of 31 days (+/-4 days) with 17 days (+/-2 days) being estrus using the vaginal cytology Animal Review method of detection. Guimarães, et al. [10] reported postpartum estrus to be 12.04 days (7-24 days). ...
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