Rapid tests for diagnosis of Leptospirosis: Current tools and emerging technologies

Institut Pasteur, Unité de Biologie des Spirochètes, National Reference Center and WHO Collaborating Center for Leptospirosis, Paris, France.
Diagnostic microbiology and infectious disease (Impact Factor: 2.46). 10/2013; 78(1). DOI: 10.1016/j.diagmicrobio.2013.09.012
Source: PubMed


Leptospirosis is an emerging zoonosis with a worldwide distribution but is more commonly found in impoverished populations in developing countries and tropical regions with frequent flooding. The rapid detection of leptospirosis is a critical step to effectively manage the disease and to control outbreaks in both human and animal populations. Therefore, there is a need for accurate and rapid diagnostic tests and appropriate surveillance and alert systems to identify outbreaks. This review describes current in-house methods and commercialized tests for the rapid diagnosis of acute leptospirosis. It focuses on diagnostic tests that can be performed with minimal training and limited equipment in less-developed and newly industrialized countries, particularly in resource-limited settings and with results in minutes to less than 4 hours. We also describe recent technological advances in the field of diagnostic tests that could allow for the development of innovative rapid tests in the near future.

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Available from: Rudy A Hartskeerl, Jul 03, 2015
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    • "From a total of 85 clinical samples, 53 sera and 5 whole blood samples (68.2%) were positive to 16S rRNA. The difference between LipL32 qPCR positivity and that of 16S rRNA PCR could be related to the limitations of the conventional PCR used in the 16S rRNA (Merien et al., 2005; Picardeau et al., 2014). Fifty five samples were grouped within L. interrogans and one sample (serum 99) within L. kirschneri. "
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    • "Patients present with clinical signs difficult to distinguish from other endemic illnesses including dengue fever, malaria, HIV, rickettsial disease and yellow fever. Further, regional and district level health centers often lack appropriate diagnostic laboratories to perform the serological testing required to establish a diagnosis [9]. "
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