Forest cover is the primary determinant of elephant distribution, thus, understanding forest loss and fragmentation is crucial for elephant conservation. We assessed deforestation and patterns of forest fragmentation between 1930 and 2020 in Chure Terai Madhesh Lanscape (CTML) which covers the entire elephant range in Nepal. Forest cover maps and fragmentation matrices were generated using multi-source data (Topographic maps and Landsat satellite images of 1930, 1975, 2000, and 2020) and spatiotemporal change was quantified. At present, 19,069 km 2 forest cover in CTML is available as the elephant habitat in Nepal. Overall, 21.5% of elephant habitat was lost between 1930 and 2020, with a larger (12.3%) forest cover loss between 1930 and 1975. Area of the large forests (Core 3) has decreased by 43.08% whereas smaller patches (Core 2, Core 1, edge and patch forests) has increased multifold between 1930 and 2020. The continued habitat loss and fragmentation probably fragmented elephant populations during the last century and made them insular with long-term ramifications for elephant conservation and human-elephant conflict. Given the substantial loss in forest cover and high levels of fragmentation, improving the resilience of elephant populations in Nepal would urgently require habitat and corridor restoration to enable the movement of elephants. Deforestation and conversion of natural areas into human use impacts the earth's ecosystems and functions, and threatens biodiversity 1, 2. The population of many wildlife species are declining globally, and about a million species are under threat of extinction primarily due to habitat loss/degradation, overexploitation, climate change, illegal wildlife trade, direct persecution, and conflict with humans 3-5. Fragmentation is a significant factor leading to the loss of biodiversity in forested landscapes 6. Habitat fragmentation affects ecological patterns and processes by increasing the number of forest patches, reducing the patch size, interrupting connectivity within the ecological network 7-9 , and impacting several species 10. Habitat fragmentation could alter animal communities and trigger cascading effects on plants and ecosystem functions, including their carbon storage potential 11-13. Continued fragmentation can lead to microclimatic changes in the edges, reduced core habitat, and eases the establishment of invasive species towards the forest interiors 14, 15. Effects of fragmentation on wide ranging large mammals like elephants is more severe and increases the extinction risks due to their needs for large and intact habitats 16-18. With the current rise in anthropogenic impacts and loss of wildlife habitats, shared heterogenous landscapes around protected areas have immense potential for long term conservation of large mammals 19, 20. Elephants are the largest living terrestrial mammals facing typical threats of large mammals such as habitat loss, poaching and conflict with communities 21. The increase in human population and expansion of agriculture had led to habitat loss and fragmentation, resulting in a significant decline in elephant populations across Asia and Africa 22-24. Asian elephants are confined to 5% of the historic elephant range 24. Elephants use large areas to meet their dietary and reproductive requirements 25, 26. Their home range size varies according to the forage OPEN