1H, 15N and 13C resonance assignments for the three LOTUS RNA binding domains of Tudor domain-containing protein TDRD7
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, 55905, USA.Biomolecular NMR Assignments (Impact Factor: 0.76). 04/2012; 7(1). DOI: 10.1007/s12104-012-9382-1
The LOTUS or OST-HTH domain is a recently discovered motif of about 80 amino acids and is found in several germline-specific proteins including the Tudor domain-containing proteins TDRD5 and TDRD7, which are important for germ cell development. The LOTUS domain is an RNA binding domain but its exact function is unknown. Here, we report the 1H, 13C and 15N resonance assignments for the three LOTUS domains present in mouse TDRD7. These assignments will allow three-dimensional structure determination of the LOTUS domains and mapping of their interaction with RNA, steps toward deciphering the function of TDRD7.
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ABSTRACT: To examine whether polymorphisms in the RNA granule component tudor domain-containing protein 7 (TDRD7) gene are associated with susceptibility to age-related cataract (ARC) in a Han Chinese population. Patients with ARC, and age-, sex- and ethnically-matched healthy control subjects were enrolled in the study. Five single nucleotide polymorphisms (SNPs) within the TDRD7 gene, rs1462091, rs11793735, rs10981985, rs2045732 and rs1462089, were genotyped using a SNaPshot® Multiplex Kit. The study included 271 control subjects and 218 patients with ARC. The rs10981985 SNP was associated with ARC in dominant (odds ratio [OR] 0.561, 95% confidence interval [CI] 0.388, 0.809) and allele dose (OR 0.619, 95% CI 0.455, 0.841) genetic models. The rs10981985 A allele frequency was lower in patients with cortical ARC than in control subjects (OR 0.502, 95% CI 0.315, 0.801). The rs10981985 polymorphism was significantly associated with cortical ARC in a dominant genetic model (OR 0.431, 95% CI 0.251, 0.740). The present study suggests that the rs10981985 G → A variant within the TDRD7 gene may protect against cortical ARC in a Han Chinese population.
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