Sublethal exposures of diazinon alters glucose homostasis in Wistar rats: Biochemical and molecular evidences of oxidative stress in adipose tissues

Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences (TUMS), Iran
Pesticide Biochemistry and Physiology (Impact Factor: 2.01). 11/2013; 105(1):57–61. DOI: 10.1016/j.pestbp.2012.11.008


Disorder of glucose homeostasis is one of the most important complications following exposure to organophosphorous (OPs) pesticides. Regarding the importance of adipose tissue in regulating blood glucose and the role of oxidative stress in toxicity of OPs and in the continue of our previous works, in the present study we focused on tumor necrosis factor alpha (TNFα), glucose transporter type 4 (GLUT4), and nuclear factor kappa-light-chain-enhancer of activated B cells (Nf-κB) in a sublethal model of toxicity by diazinon as a common OPs.

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    • "The rate of oxidation from DCFH to dichlorofluorescein (DCF) indicative of oxidant production was followed at the excitation wavelength of 488 nm and emission wavelength of 525 nm and measured every 6 min for 60 min using an ELISA F-2000 fluorescence spectrometer. The rate was linear for at least 60 min at various concentrations of protein present, corrected for the autooxidation rate of DCFH [20] "
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