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In the recent years synthetic drugs have been widely replaced with herbal medicines and plant extracts because of their little undesirable and extensive beneficial effects. Plantago majorL. (also known as plantain and way bread) is a member of the Plantaginaceae family. Leaves and seeds of the plant have been widely used in folk medicine for various purposes, including treatment of an extensive range of diseases and disorders such as respiratory complications and digestive system affections. It has been also used in wound healing and as an antiinflammatory, antimicrobial and antitumor agent. Moreover, plantain contains ingredients which can neutralize internal and external poisons. Recent studies have also shown its anti-fatigue properties. Phytochemical analysis of P. major extract has indicated that this plant contains a wide range of chemicals such as polysaccharides, lipids (saturated and non-saturated), amino acids (essential and non-essential), caffeic acid derivatives, flavonoids, iridoidglycosides and terpenoids, which have the potential to exert different biological effects. Phenols (ferulic acid), flavonoids and tannins have the highest amount in Plantago leaves. The present review describes the traditional uses and recent findings(Since 2000 till date) about the pharmacological effects of Plantago major L.
J. Basic. Appl. Sci. Res., 3(9)xxxx-xxx, 2013
© 2013, TextRoad Publication
ISSN 2090-4304
Journal of Basic and Applied
Scientific Research
*Corresponding author: Milad Moloudizargari, Student of Veterinary Medicine, Faculty of Veterinary Medicine, Urmia
University, Urmia, Iran. Email: Postal code: 5716114999
Phone number: +989141482523
Therapeutic Uses and Pharmacological Properties of Plantago major L. and
its Active Constituents
Ali Nazarizadeh
, Peyman Mikaili
, Milad Moloudizargari
*, Shahin Aghajanshakeri
Soheil Javaherypour
Department of Pharmacology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
Student of Veterinary Medicine, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Inthe recent years synthetic drugs have been widely replaced with herbal medicines and plant extracts because
of their little undesirable and extensive beneficial effects. Plantago majorL. (also known as plantain and way
bread) is a member of the Plantaginaceae family. Leaves and seeds of the plant have been widely used in folk
medicine for various purposes, including treatment of an extensive range of diseases and disorders such as
respiratory complications and digestive system affections. It has been also used in wound healing and as an anti-
inflammatory, antimicrobial and antitumor agent. Moreover, plantain contains ingredients which can neutralize
internal and external poisons. Recent studies have also shown its anti-fatigue properties. Phytochemical analysis
ofP. major extract has indicated that this plant contains a wide range of chemicals such as polysaccharides,
lipids (saturated and non-saturated), amino acids (essential and non-essential), caffeic acid derivatives,
flavonoids, iridoidglycosides and terpenoids, which have the potential to exert different biological effects.
Phenols (ferulic acid), flavonoids and tannins have the highest amount in Plantagoleaves. The present review
describes the traditional uses and recent findings(Since 2000 till date) about the pharmacological effects of
Plantago major L.
KEY WORDS: Plantago major L.; Plantain; Ferulic acid; Plantaginaceae; Wound healing.
Plantago a member species of the Plantaginacea family. It is an herbaceous perennial with a
rosette of leaves15-30 cmin diameter. Each leaf is oval, 5-20 cm long and 4-9 cm broad, rarely up to 30 cm long
and 17 cm broad, with an acute apex and a smooth margin; there are five to nine conspicuous veins. The seeds
are quite small with an ovate shape (0.4–0.8_0.81.5 mm) and a slightly bitter taste (Samuelsen, 2000). The
flowers are small, greenish-brown with purple stamens, produced in a dense spike 5-15 cm long on top of a stem
13-15 cm tall (rarely to 70 cm tall) (Fig. 1). It grows better than the most of other plants in compacted soils, and
is abundant beside paths, roadsides, and other areas with frequent soil compaction. It is also common in
grasslands and as a weed in crops. It is wind-pollinated, and propagates primarily by seeds, which are held on
the long, narrow spikes which rise well above the foliage (Blamey and Grey-Wilson, 1989). The plant is native
to the most of Europe and Northern and Central Asia, and is widely naturalized elsewhere in the world, where it
is a common weed. P. majorwas spread by man from Europe throughout the world4000 years ago (Jonsson,
1983).The Indiansnamed it ‘White man’s footprint’ because it was found everywhere the Europeans had been.
This has been adapted into the genus name Plantago that is from Latin planta, meaning sole of the foot. Plantain
was also used in the time of Shakespeare and was also named in the piece "Romeo and Juliet" Act I, Scene II of
the period 1592 to 1609(Samuelsen, 2000).The plant is well known in many countries (especially in Iran) and
has been used extensively in folk medicine because of its various beneficial effects (table1).Native Americans
carried powdered roots of P. major as protection against snake bite or to ward off snake. As traditional Chinese
medicine, P. major has long been used for treating viral related disease from colds and influenza to viral
hepatitis (Chiang et al., 2002).Studies carried out on the chemical composition of the plant by various methods
(for example: simple, rapid and accurate high-performance liquid chromatography) show extensive chemical
components (tables 2). Studies conducted by Jamilahet al. (2012) on the chemical composition of various
extract (petroleum ether, methanol, ethyl acetate, n-butanol and aqueous) from P. major leaves showed that all
of them have phenol groups in their extract while having different variation of organic acid groups, flavonoids
and terpenoids. P. major leaves contain 0.07% of oleanolic acid and 0.22%of ursolic acid which are two major
terpenoids of the plant(Tarvainenet al., 2009;Samuelsen, 2000)(Fig 2).These compounds exist in almost all parts
of the plant. Bioactivity ofP. major leaves and other herbal preparations which contain these secondary
metabolites is attributed to these chemical constituents (Liu, 1995).It has been shown in one study that
ursolicacid from P. majoris a Selective Inhibitor of Cyclooxygenase-2 catalyzed prostaglandin biosynthesis;
hence, anti-inflammatory effect of the plant is possibly via this mechanism. So many other studies are found in
Mikaili et al., 2013
the literature on the pharmacological effects of these two important chemicals. Leaves of the plant are rich
sources of essential fatty acids (18:26 and 18:33)and also of carotenes (Guil-Guerrero and Rodríguez-
García, 1999).Ferulic acid which has high amounts in plantago (especially in seeds)is a hydroxyl cinnamic acid,
a type of organic compound(Mohamed et al., 2011). It is an abundant phenolic phytochemical found among
plant cell wall components (Fig.2). Ferulic acid, like many natural phenols, is an antioxidant in vitro. Recentlyin
vitro and animal model studies have suggested that ferulic acid may have direct antitumor activity against breast
and liver cancer (Valentão et al., 2001; Pierre et al., 2006).Dosage range is 3-5 g of the powdered herb 1-3 time
daily but the most common dose as infusion is 150 ml (one cupful) 3-4 time a day. All in all the plant is safe and
there are no limitations and drug contraindications reported so far; however, enough care should be taken
regarding usage of the plant in pregnant women(Zagari, 1992).
This article reviews the traditional and newly investigated uses of P. major L. along with its
pharmacological effects.
Figure 1.P. major L. (leaves and seeds).
Figure 2. Chemical structures of UA and OA
Name Chemical structure
Ursolic acid
Oleanolic acid
Ferulic acid
J. Basic. Appl. Sci. Res., 3(9)xxxx-xxx, 2013
Table 1. Traditional uses of Plantago major L. in different countries especially Iran.
Part of plant and preparation Usage Country References
Whole plant decoction
Healing different kinds of wounds such as
(snake bite, intestinal worms and infectious
wounds), cold treating,Remedy for diabetes
Colombia, Italia
Watkins et al., 2011.
Idoloet al., 2010.
Jaraldet al., 2008.
Fresh leaf of the plant
Internal inflammations such as cystitis,
enteritis and swollen abdomen
Watkins et al., 2011.
Internal use of leaves(oral)
Respiratory catarrh;astringent effect;
bleeding, skin problems; eye inflammations;
also fresh leaves applied to treat livestock
hematomas and their skin problems;pruritus.
Colombia, Iran,
Rahimiet al., 2010.
Mir-heidari, 1994.
Zagari, 1992.
Neveset al., 2009.
Topical use of leaves (lotion)
rectifier, Kidney pain
Portugal, Italia, Iran
Viegiet al., 2003.
Idoloet al., 2010.
Zagari, 1992.
Mix of Leaf and Root
Mir-heidari, 1994.Zagari,
Decoction and infusion of fresh leaf Kidney pain
Boulogne et al., 2011.
Decoction of leaves of
Plantago major
Remedy for hemorrhagic-diarrheal, Tonic,
Central America and
Vera-Kuaet al., 2010.
Seeds of plant
mouth inflammation
Eye inflammation
Iran, India
Mir-heidari, 1994.
Oral use of the extract
Remedy for tuberculosis
Soothing effect
Mir-heidari, 1994.
Zagari, 1992.
Decoction of root Decoction of P.
Remedy for pulmonary disease
Mir-heidari, 1994.
heidari, 1994.
Decoction of leaf
with vinegar
Mir-heidari, 1994.
Mixture of the sap of leaves and
Remedy for Ear pain and Bruises
Mir-heidari, 1994.
Extract of the root Urinary tract infection; toothache Iran Mir-heidari, 1994.
Decoction of P. major,
Euphorbia schlechtendalii
Stomatitis, asthma, bronchitis, ear ache, anti-
Iran , Central America
and Mexico
Vera-Kuaet al., 2010.
Remedy for Ear pain
heidari, 1994.
Whole plant
Root of plant
Aqueous extract
Urinary tract, toothache
Stomatitis, Asthma, bronchitis, Ear ache
Zagari, 1992.
Zagari, 1992.
Zagari, 1992.
Juice of the plant and honey Anti tussive Iran Zagari, 1992.
Table2. Biological active compounds of P. major L. leaves and seeds (mg/g on dry weight basis)
et al.
, 2011)
P. major L.
P. major L. leaves
7.43±0.07 13.05±0.10 Total phenol (mg gallic/g)
Total flavonoid (mg Quercetin/g)
2.43±0.03 5.63±0.06 Tannins (mg Catechine /g)
Immune Enhancing Effects
Endotoxin-free methanol extracts ofP.majorleaves, in the absence of IFN- or LPS, increased production
of nitric oxide (NO) andTNF-by rat peritoneal macrophages andstimulated lymphocyte proliferation in a dose-
dependentfashion.NO and TNF- production by untreated macrophages was negligible.The regulation of
immune parameters by the extract of P. majormay be helpful in treatment of numerous diseases (Gomez-
Floreset al., 2000).For instance, activated macrophages produce mediators of cytotoxicity such as nitric oxide
and tumour necrosis factor-alpha ( TNF-α), kinds of lymphokines which protect the host against the
development of tumors and infections by organisms such as Cryptococcus, Schistosoma, Leishmania,
Francisella, Listeria and Mycobacteria (Nathan and Hibbs, 1991;Hibbset al., 1988.)
Hepatoprotective Effects
Hepatic disorders have grown in recent years and are the cause of billions of deaths all over the world
(Williams, 2006). In one study the hepatoprotective activity of P. majorseed extract in an experimental rat
model of carbon tetrachloride (CCl
) induced hepatotoxicity was evaluated. Control, CCl
and reference groups
received isotonic saline solution, CCl
and silibinin, respectively. P. major groups were injected CCl
ml/kg) and the extract at doses of 10, 20 and 25 mg/kg, respectively for seven days. After sacrificing animalson
Mikaili et al., 2013
the 8th day blood and liver samples were collected, and then changes in plasma marker enzymes of hepatic
damage and histopatholgical alterations were recorded. Significant decrement in alanine aminotransferase
(ALT) and aspartate aminotransferase (AST) levels was observed at the dose of (25 mg/kg) in the treated group.
Microscopic examination of the livers depicted significant recovery and minimization of necrobiotic changes, in
same group (Türelet al., 2009). Parallel study was carried out by Attaet al. (2006).The results of these studies
indicate potential hepatoprotective effect of P. major extract. Further studies may suggest replacement of
synthetic drug with this natural preparation.
Anti-ulcerogenic Effects
According to some ancient evidence, major seeds have been used as a remedy for inflammatory bowel
disease (IBD) in Iran, butthere are no or not enough studies supporting this use of the plant (Rahimiet al.,
2010).In screeninganti-ulcerogenic activity of P. major, methanol extract of the plant was assayed using ethanol
and aspirin-induced gastric ulcerationsinrat models. Leaf and seed extracts were prepared separately to observe
the difference in their pharmacological actions. In alcohol induced ulcer models, the leaf extract significantly
decreased the ulcer index with curative ratio of 87.50% but the seed extract had no considerable effect in the
same model. Ranitidine (100mg/kg) produced curative ratio of aspirin-induced ulcer models. Leaf
extract at an oral dose of 400 mg/kg, significantly decreased the number of gastric ulcers; however, the seed
extract had no effect on the number of ulcers. Both extracts decreased total acidity. It was also observed that
P.major (leaves and seeds) extract at an oral dose of 400mg/kg did not affect the volume of gastric juice or its
protein content. No details have been revealed about the mechanisms of these actions(Atta et al., 2005).Also one
of the main ulcerogenic agents in digestive system is Helicobacter pylori. According to Cogoet al. (2010)
studies Plantago major is capable to inhibit H. pylori in vitro.
Antidiarrheal Effects
In a study, the effect of ethanolicextract of PlantagoLeaves was evaluated on castor oil-induced diarrhea
andgastrointestinal movements in rats (charcoal meal) and on the motility of duodenum isolated from freshly
slaughtered rabbits.P. majoratadose of 200mg/kg (oral) demonstrated significant antidiarrheal effect for at least
4h.This activity was potentiated at a dose of 400mg/kg (oral). In addition, the extract significantly decreased the
distance travelledby the charcoal meal when given at both doses (200and 400 mg/kg).The large dose of the plant
extract was slightly more effective than the small one. The extract in a concentration of1.6 mg/mL or less
produced a transient stimulation the on motility of isolated duodenum. A higher concentration produced rapid
relaxation. The initial stimulant effect may be attributed to the presence of irritant substances and may explain
the contradiction in the folkloric use of this plant. No clear details were registered about responsible ingredients
(Atta and Mouneir, 2005).Further research is, however, needed to determine what compounds are responsible
for the Antidiarrheal effect.
Antinociceptive Effects
Methanolic extracts of leaves and seeds separately were studied on acetic acid-induced writhing and tail-
flicktestin mice, to investigate their anti-nociceptive effects. Oral administration of 400 mg/kg of the seed
extract showed significant nociceptive activity against acetic acid-induced writhes with a protection of62.3%.
However, at same dosesthe protection rate of the leaf extract was only 48.8%.These values were compared to
80.5% for the standard dipyrone (50 mg/kg) which is the synthetic drug. The smaller dose (200 mg/kg) of the
plant extract did not protect animals from painful acetic acid stimulation. The leaf extract at the dose of
400mg/kgproduced significant increase in the latency to the tail response to thermal stimulation. Mild or no
effect was observed at the small dose. No detail study has been carried out so far, about the ingredients that can
induce such analgesic effect (Atta and Abo EL-Sooud, 2004).
Antioxidant and Free Radical Scavenging Effects
Free radicals contribute to more than one hundred disorders in humans including atherosclerosis, arthritis,
and ischemia and reperfusion injury of many tissues, central nervous system injury, gastritis, cancer and AIDS
(Kumpulainen and Salonen, 1999; Cook and Samman, 1996).Free radical scavengers are any compounds that
react with free radicals in a biological system, thus reducing free radical-induced damage and protecting against
the indirect effects of free radicals. Ethanolic, hot and cold water extracts of P. major leaves and seeds were
assayed for determination of free radical-scavenging activity using stable 1, 1-diphenyl-2-picryl hydrazyl radical
(DPPH)in vitro. Highest antioxidant activity was observed with the ethanolic leaf extract even at a low
concentration of 20 ppm (78% activity). In the same concentration the ethanolic seed extract had really low
activity (25%). The antioxidant activity of both mentioned extracts increased in a concentration-dependent
fashion, up to 60 ppm. The rate of antioxidant activity for both extracts was so close in a concentration of
100ppm. Hot and cold water extracts of Plantago leaves were more effective than the seed extract. It was also
observed that, the ethanolic extracts were more active than the hot and cold water extracts of the samples under
J. Basic. Appl. Sci. Res., 3(9)xxxx-xxx, 2013
et al.
, 2011). In another study, methanolic extract of the whole plant was studied to
measure the antioxidant activity and determine responsible contents. Results showed that the scavenging
property against DPPH, is probably due to hydroxyl groups existing in the phenolic compounds of the plant.
The extractata concentration of 0.8 mg/g had 89.3±1.5% scavenging activity and with an IC50of 0.32mg/ml.
Totalphenol content of the plant, responsible for the scavenging activity, was measured as 31±4mg/g
et al.
, 2006).
Another suggested mechanism responsible for the scavenging activity of P. major is that
highly active flavonoids present in the plant possess a 3',4'-dihydroxy occupied B ring and/or 3-OH group which
can embrace oxygen radicals and reduce them into neutralized substances like water (Amicet al., 2003).
Anticancer Effect
In one study, methanolic extracts from seven
speciesused in traditional medicine among
P. major
, were evaluated for cytotoxicactivity against three human cancer cell lines:
human renal
adenocarcinoma(TK-10), the human breast adenocarcinoma (MCF-7) and the human melanoma (UACC-
62) cell lines) using the sulphorhodamine B (SRB) assay
in vitro
P. major
and the other six
species showed cytotoxic activity on the breast adenocarcinoma (MCF-7) and melanoma (UACC-62)
tumoral cell lines in a concentration-dependent manner at the recommended NCI (USA) doses. None of the
extracts, with the exception of
(GI50 = 86
g/ml), showed cytotoxic activity against renal
adenocarcinoma (TK-10) cells. It is thought that the cytotoxic activity depends basically on flavonoids,
flavone and luteolin present in the extract (Gálvez
et al.
, 2003).
in vitro
study was carried out on
ethanolic, hot and cold water extracts of leaves and
seeds separately. A dose dependent inhibition was observed for all testedextracts. The ethanolic extract of
P. major
L. leaves had the greatest effect on tumor cell growth (Dead 74% ±0.35) follow by its hot water
extract of the leaves (Dead 54.6% ± 1.21)(Mohamed
et al.
, 2011)
.Luteolin-7-O- β-glucoside, the major
flavonoid found in all species of Plantago, is known to be the responsible agent for the anti-cancer activity of the
plant.The precise mechanism responsible for the cytotoxic activity of luteolin-7-O- β-glucosideis not thoroughly
understood, however it is thought that topoisomerase-mediated DNA damage is the involved mechanism.
Luteolin-7-O-β-glucoside acts as a potent DNA topoisomerase I poison as well as its aglyconluteolin. (Gálvezet
al., 2003)
Cytotoxic Activity
The cytotoxic activity of P. major methanol extract on human transformed cells: HCT-15
(colon carcinoma), SQC-UISO (cervical carcinoma), OVCAR (ovary carcinoma) and KB
(nasopharynx carcinoma) cultured in RPMI-1640 medium has been also evaluated, in vitro. The
extract (1µg/mL) was cytotoxic against the UISO and OVCAR cell lines but stimulated the
proliferation of KB cells. (Velasco- Lezamaet al., 2006).
Ina screening of anticancer effect of forty-
five Russian plants, used in folk medicine, a parallel
in vitro
study was carried out using Mouse leukemia
cells (L1210).Methanolic extract of
had 80-100% cytotoxic effect (Goun
et al.
, 2002). Similar
work was done in Vietnameseandseventy-seven medicinal plants tested for their antiproliferative activities
against human HT-1080 fibro sarcoma cells.
P. major
was not among the most active plants (Ueda, 2002).
Studies on the efficacy of hot water extract of
P. major
leaves on Ehrlich ascites tumors in male mice were
also undertaken.
The extract was most effective at a dose of
25µg/mlagainst the tumor cells. The results
show that
P. major
couldbe proposed as an effective agent in cancer prevention (Ozaslan
et al.
, 2009).
Hematopoietic Effects
Aqueous and methanolic extracts of the aerial partsof P. major were added to bone marrow and spleen cell
medium to investigate their hematopoietic potential. The results were as following:
Bone marrow cultures: The aqueous and methanolic extracts stimulated cell proliferationin similar manner
using a dose of 0.4and 0.2 gr/mL.Maximum hematopoietic activity was observed at0.1 and 0.05 g/mL doses of
the methanolicextract(Velasco-Lezama et al., 2005).
Spleen Cultures: Doses of 0.4 and 0.2 g/mL of the aqueous extract increased thecell population by 3.30- and
4.40-fold, respectively. The same concentrations of the methanolic extract increased the population by 6.25- and
4.28-fold, respectively. The increase was significantly higher in spleen cultures than in bone marrow
cultures(Velasco-Lezamaet al., 2005).This effect of P. majoron spleen as a hematopoietic organ is thought to be
the second mechanism through which the plant exerts hematopoietic effects.
Wound Healing Effects
Use of
P. major
in wound healing has a very long history. Greekphysicians described its wound
healing activity in the first century and the
leaves were used as a remedy for dog bites (Samuelsen, 2000;
Roca-Garcia, 1972).It is also well known for its wound healing property in Scandinavia
. The common
Norwegian and Swedish name for
P. major
meaning "healing leaves"
(Samuelsen, 2000). Hence,
Mikaili et al., 2013
the plant`s Water (fresh and dried leaves) and ethanol-based leaves extracts were studied using scratch assay
with the oral epithelial cellsin vitro to validate
ancient traditional use. Concentrations of 0.1, 1 and 10mg/ml
of the plant extracts added to cell culture media to observe cell proliferation/migration. Apart from the highest
concentration of 10mg/mL, ethanol-based extracts had the most beneficial effect, followed by water extracts of
fresh leaves, ethanol plus water extracts of dried leaves and, finally, water extracts of dried leaves. Maybe
polyphenols are the responsible compounds for wound healing. Phytochemical analysis showed that high levels
of plantamajoside and other polyphenols exist in ethanol-based extract compare with other tested extracts. Other
involved substances are polysaccharides (Zubairet al., 2012).In a whole, a mixture of antioxidants are said to be
effective the wound healing process of the plant (Yokozawaet al., 1997).
Anti-inflammatory Effects
Inflammation is a complex event linked to tissue damage whether by bacteria, physical trauma,
chemical, heat or any other phenomenon and inflammatory response is the critical protective reaction to
these kinds of injures remarked by redness, fever, oedema (swelling) and pain of involved tissue (Morais
et al.
, 2011; Levine and Reichling, 1999). Methanol extract of
P. major
L. seeds was assayed on
carrageenan-induced rat paw oedema to evaluate the anti-inflammatory activity.
P. major
showed anti-
inflammatory effect in a dose dependent fashion, but it was not more effective than indomethacin
(reference drug).Median effective dose (ED
)was determined to be 7.507 mg/kg (
Türelet al., 2009).
It could
be thought that inhibition of COX-2-catalyzed prostaglandin biosynthesis may be the involved mechanism
for the anti-inflammatory action (Ringbom
et al.
, 1998). Furthermore, flavonoid derivatives which are high
P. major
are other responsible constituents present in the plant (Middleton
et al.
, 2000;Havsteen, 2002).
Fatigue is a condition which is marked by the feeling of
due to heavy physical activity and
generally can cause muscular pain. Ethanol extract of
P. major
seeds were studied on forty eight male mice
to determine its effect on physical strength. Forced swimming test and biochemical assays of blood were
carried out and marker factors were registered. According to the results, the extract increased swimming
time by increasing tissue glycogen (as energy source)and decreasing serum urea nitrogen and blood lactate
(as fatigue agents).Therefore, it is suggested that the extract possesses anti-fatigue effects and can improve
endurance exercise capacity (Mao-yeand and Li-guo, 2011).There is little evidence about this effect of
and the precise mechanisms responsible, therefore more studies are required to be conducted in this
Pest Organism Managing Properties
An ethno medicinal survey was applied in the city of Pelotas, Brazil, with professionals and patients
in the Unified Health System (SUS), showed that the most frequent symptom reported for herbal drug
usage was infection (55.3%), and tanc¸ agem (
P. major
) was the plant mentioned to be the most often used
to treat this problem (37.3%) (Dias Oliveira
et al.
, 2012).
In order to evaluate efficacy of mayan (
a large family of American Indian)
traditional potions in
treating of infectious bowel disease
P. major
L. and thirty-eight other plants were studied
in vitro
bacteria including
Escherichia coli
Klebsiella pneumonia
Shigella flexneri
Salmonella typhi
and protozoa
such as
Entamoeba histolytica
Giardia lamblia
. Mixture of
Melochia nodiflora
P. major
was active against
Giardia lamblia
= 21.78
g/ml). The efficacy of this
formulation for both protozoa was comparable with positive control (metronidazole). Moreover, another
formula composed of four herbs,
Trema micrantha
Euphorbia schlechtendali
Diphysa carthagenensis
P. major
showed good activity against
Giardia lamblia
with an of IC
= 12.71
g/ml. Both formulae
P. major
were so close to the efficacy index of metronidazole against
Giardia lamblia
. These two formulae showed no considerable effect against bacteria (Vera-Ku
et al.
, 2010). In
another study, extracts of 13 Brazilian medicinal plants were screened for their antimicrobial activity
against bacteria and yeasts. Leaves extract of
P. major
presented some degrees of antibacterial activity and
was not among the most active plants (Holetz
et al.
, 2002).
Similar works were carried out by SaltanÇitoğlu and Altanlar (2003), but the results were adverse.
According to this study, when compared with the standard antibiotics,
P. major
extract was found to have
good activities against
E. coli
S. aureus
. However,
P. major
did not show any activity against
. Further details are available in table 3.
J. Basic. Appl. Sci. Res., 3(9)xxxx-xxx, 2013
Table3. The inhibition zones diameters of free and aqueous ethanolic extracts of P. major (mm) (Çitoglu and
Altanlar, 2003).
N.T.: not tested. (-): no inhibition zone, Ampicillin and Fluconazole are positive control.
Antibacterial effects of acetone and ethyl alcohol extracts of
P. major
L. leaves were studied, using
macro dilution liquid (tube) method. Both extracts were tested for nine bacteria species (
Bacillus cereus,
Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella
pneumonia, Pseudomonas aeruginosa, Proteus mirabilis, and Salmonella enteritidis
). The ethyl alcohol
extract was only effective against
E. coli
B. cereus
, but acetone extract was effective on all selected
bacteria species at different concentrations (Metiner
et al.
, 2012).
Previously, parallel studies were carried out by Sharifa
et al.
(2009). The whole plant methanolic,
ethanolic and aqueous extracts of
P. major
were tested on S
taphylococcus aureus, Bacillus subtilis,
Escherichia coli, Candida albicans
Candida tropicalis
. The methanolic and ethanolic extracts at
concentrations of 100-200 mg/ml showed bactericidal activity against both Gram positive and Gram
negative bacteria tested. Electron microscopic observation demonstrated collapse in Gram positive bacteria
cell wall and blebs formations on Gram negative bacteria. No activity was observed against yeast by any of
the extracts.
In one study, the aqueous, methanol, chloroform and hexane extracts of the aerial parts (leaves and seeds)
of P. major were added to Escherichia coli, Bacillus subtilis and Candida albicans cultures and antibacterial
activities were observed in different ranges (Velasco-Lezama et al., 2005).
The antibacterial effect of a soluble pectin polysaccharide (PMII), isolated from the leaves of P. major
was examined against systemic Streptococcus pneumoniae serotype 6B using animal model of mice. It was
observed that PMII can have prophylactically protective effects (Hetland et al., 2000)
Antiviral activity of aqueous extract and pure compounds of P. major was assayed using herpes viruses
(HSV-1, HSV-2) and adenovirus species (ADV-3, ADV-8, ADV-11).The aqueous extract possessed only a
slight anti-herpes activity. In contrast, the pure compounds were active against selected viruses. Among them
caffeic acid exhibited the strongest activity against HSV-1 (EC
=15.3 g/ml, SI=671), HSV-2 (EC
=87.3 g/ml,
SI=118) and ADV-3 (EC
=14.2 g/ml, SI=727), whereas chlorogenic acid possessed the strongest anti-ADV-11
=13.3 g/ml, SI=301) activity. The potency relates to the presence of hydroxyl group in the chemical
structure. Compounds that contained hydroxyl groups at the R
and R
positions (caffeic acid, chlorogenic acid)
were more potent than the compounds containing one hydroxyl group at the R
position (ferulicacid, p-coumaric
acid). Results suggest the use of these compounds as a remedy for infections caused by these two viruses
(Chiang et al., 2002). Results are in contrast with those reported by McCutcheon et al. (1995).
According to a pilot study by Ali et al. (2004), interview with 492 informants from 13 villages indicated
that macerated, dried leaves of P. major can be used as a remedy for malaria. Screening study must be carried
out to determine antimalarial activity of P. major and also to isolate and identify the active compounds, which
may be regarded as future promising phytotherapeutics in the treatment of malaria.
Antigenotoxic Effects
Anti genotoxic property of the sap from greater plantain (P. major L.) was investigated using two
bacterial test systems (SOS chromo test and Rec assay). P. major showed sizable anti genotoxic effect in none
of the test systems. This means that P. major extract cannot be used as a dis mutagen (prevent DNA damage) or
bio anti mutagen (repair of damaged DNA) agent (Karamova et al., 2010).
External Poison Detoxification
Heavy metals such as lead are toxic for humans and animals and can cause various diseases. P. major L.
was grown hydroponically in a water medium supplemented with concentrations of lead ion under different
duration times and temperature regimes to evaluate the efficacy of lead detoxification by different parts of the
plant (roots, stems, leaves and whole plant). Roots of the plant showed the highest removal rate of lead than
other parts (Akram et al., 2007). As a concern in public health, the use of agents which can purify water and
environment from heavy metals is necessary. Therefore, P. major can act as a bio filter for the removal of
Pbcations from water.
Diameters of the inhibition zones (mm)
E.coli P.aeruginosa B. subtilis S. aureus C.albicans C.galabrata C. krusei
Plantago major
Ampicillin (25 fig) 12 N.T 13 15 N.T N.T N.T
Fluconazole(25 ng) N.T N.T N.T N.T 18 20 20
Mikaili et al., 2013
Toxicity Evaluation
In recent years synthetic drugs have been widely replaced with herbal medicines in both developed and
non-developed countries (Verma and Singh, 2010). Some possible reasons are: the development of new diseases
with severe complications for which there is still no appropriate treatment and the belief that herbal medicine
have less or no side effects. In addition to the belief that herbal medicine is naturally superior to synthetic drugs,
economically plants are cheaper sources of remedies (Capasso et al., 2000). Apart from advantages of herbal
drugs, their safety has not been confirmed scientifically and there are still some risks in their usage. Presence of
toxic constituents (pyrrolizidine alkaloids, saponins, cyanogenetic glycosides, etc.) is the main risk in this
regard. Evaluation of toxicity of chemical and natural products isolated from 20 plants was carried out using
Artemia salina L. (Artemiidae) as in vitro test and Swiss albino mice as in vivo test. LC
and LD
for P. major
were determined as 4.74 (µg/mL) and 182.54 (mg/kg), respectively. P. major was not among the most toxic
plants (Parra et al., 2001). Analysis of the anti-nutritional and toxic components showed low content of oxalic
acid (6736 mg) and erucic (3.45%) in P. major extract (Guilet al., 1997). Laboratory studies have reported
uterine stimulatory activity of P. major; therefore it should be only used under medical supervision during
pregnancy (Shipochliev, 1981). In conclusion, P. major l is a safe plant, with low content of toxic factors,
however some adverse reactions such as: nausea, vomiting, diarrhea, anorexia, bloating, hyper-sensitivity and
dermatitis may arise after treatment with the plant. Life threatening anaphylaxis may occur in more serious
cases, which is observed in high dose usage.
Conclusion and Further Scope
This review presents up to date findings about P. major, based on the most recent pharmacological
studies that support its traditional uses. The leaf extract is reliably nontoxic with strong hepato-protective and
wound healing activities, however data about the responsible constituents is little and further research is
required. Anti-fatigue effect of the plant is also one of the newly investigated effects of P. major that needs to be
further investigated.
We are grateful to thank Mr. H. Jafari Namin for his abundant support. Special thanks to Mr. Bagheri for
helping us in the preparation of table 1.
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... Plantago major L. (common plantain) is a perennial herbaceous plant belonging to the family Plantaginaceae (Samuelsen, 2000). It is a weed that grows better in compacted soils than most other plants, and it is commonly found along walkways and roadsides and in grasslands, farms, and other sites where soil compaction is common (Nazarizadeh et al., 2013;Burger et al., 2019). It has a wide geographical distribution and is clearly identifiable. ...
... The present study demonstrated stronger antibacterial activity of the ethanolic extract of P. major leaves from the habitat field edges against S. aureus and P. aeruginosa than against other microbes; these results are in agreement with those reported by Kahyaoğlu et al. (2008) and Stanisavljevic et al. (2008). However, weak or no antibacterial activity of the ethanolic extract of P. major leaves against S. aureus and P. aeruginosa was reported by Samuelsen (2000) and Nazarizadeh et al. (2013), respectively. ...
... Plantago major L. (common plantain) is a perennial herbaceous plant belonging to the family Plantaginaceae (Samuelsen, 2000). It is a weed that grows better in compacted soils than most other plants, and it is commonly found along walkways and roadsides and in grasslands, farms, and other sites where soil compaction is common (Nazarizadeh et al., 2013;Burger et al., 2019). It has a wide geographical distribution and is clearly identifiable. ...
... The present study demonstrated stronger antibacterial activity of the ethanolic extract of P. major leaves from the habitat field edges against S. aureus and P. aeruginosa than against other microbes; these results are in agreement with those reported by Kahyaoğlu et al. (2008) and Stanisavljevic et al. (2008). However, weak or no antibacterial activity of the ethanolic extract of P. major leaves against S. aureus and P. aeruginosa was reported by Samuelsen (2000) and Nazarizadeh et al. (2013), respectively. ...
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Plantago major has been used to treat a variety of diseases since ancient times. The current study aimed at assessing seasonal and habitat variations in secondary metabolites production and antimicrobial activity of p. major leave extracts. Eight habitats were selected for sampling plants during four seasons. The phytochemical screening of the ethanolic and chloroform extracts of P. major in the different habitats during different seasons showed the presence of cardiac glycosides, flavonoids and phenolic compounds. Highest cardiac glycoside and total flavonoid contents were recorded in urban and cultivated crops during winter, while the highest total phenolics was in fallow lands during summer. The separated and identified phenolic compounds using HPLC were ellagic acid, catechol, resorcinol, gallic acid and phloroglucinol, while separated and identified flavonoid compounds were: apigenin, luteolin, chrysoeriol, rutin, quercetin, kaempferol and avicularin. Methanolic extract of plants from canal bank habitat has highest effect against P. aeruginosa, followed by that of plants from orchard extract against S. aureus compared to water and ethanolic extracts. The study concluded that Plantago major contains phenolic and flavonoids which have medical uses. In addition to methanolic extract which has the highest antimicrobial activity differed with different habitats. Therefore the present study confirms the previous studies and indicated that the phytochemicals and the antimicrobial activity of the different extracts of the plant differed with difference in the ecological factors (habitat and seasons). The phytochemical/s have antimicrobial activity must be extracted, separated, purified, identified and tested as a pure or mixed
... El Plantago major se describe como una planta segura y poco tóxica, sin embargo, se han reportado algunas reacciones adversas tales como náuseas, vómitos, diarrea, anorexia, hipersensibilidad y dermatitis. (9) Hasta el momento del presente reporte, no se ha descrito una asociación con hipocalemia. (9) Aunque no hay estudios que evalúan la seguridad del uso prolongado de Plantago major en humanos, un estudio en ratas con 90 días de seguimiento no encontró cambios hematológicos, bioquímicos u otros cambios. ...
... (9) Hasta el momento del presente reporte, no se ha descrito una asociación con hipocalemia. (9) Aunque no hay estudios que evalúan la seguridad del uso prolongado de Plantago major en humanos, un estudio en ratas con 90 días de seguimiento no encontró cambios hematológicos, bioquímicos u otros cambios. (10) El Plantago major es muy versátil y tiene una serie de presentaciones tales como cápsulas, píldoras y tabletas. ...
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Introducción: El uso de plantas medicinales se asocia a la creencia de ausencia de efectos adversos. Objetivo: Reportar una posible asociación entre el uso de Plantago major e hipocalemia Presentación de caso: Paciente mujer de 38 años que acudió con entumecimiento y debilidad en extremidades debido a hipocalemia (2.94 meq/l) asociada al uso de Plantago major (Llantén), una planta medicinal de uso generalizado en Perú y de distribución global. El potasio se normalizó y los síntomas disminuyeron después de descontinuar el Llantén. Conclusión: El empleo de plantas medicinales de uso común debe tener en cuenta siempre posibles efectos adversos. Palabras clave: hipocalemia, plantago, fitoterapia, plantas medicinales
... Naturally found in Europe and Asia, the plant consists of numerous chemical components. This large number of chemical components, may explain why the plant causes irritant dermatitis(2). Plantago major leaves promotes anti-inflammatory, antimicrobial, antitumor, immunomodulatory, and wound healing effects(1). ...
... The use of plant essential oils and extracts as biopresevative to inhibit growth of pathogenic and foodspoilage bacteria is of great interest in dried seafood industry owing to harmless side effects, broad spectrum of antibacterial activity, economic benefits and a long history of safe use in foods (Nazarizadeh et al., 2013). However, high concentration of plant extracts/essential Nimrat et al. 513 oils required to achieve satisfactory inhibitory effects in food matrix as observed in in vitro experiment may be problematic because of degenerative sensorial quality of food products and unacceptability of consumers (Burt, 2004). ...
... Pentacyclic triterpenoids have been proposed as one of the main constituents responsible for biological activities of Plantago species such as cytotoxicity (Galvez et al. 2003), anti-inflammatory (Samuelsen 2000;Hussan et al. 2015), immunomodulatory, antioxidant (Najafian et al. 2018), and anticancer (Kartini et al. 2017). Among them, ursolic acid (UA) is the major pentacyclic triterpenoid obtained from Plantago leaves having diverse pharmacological properties and is known to inhibit cyclooxygenases and therefore, possesses anticancerous, anti-inflammatory activities (Nazarizadeh et al. 2013). ...
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Plantagos are important economical and medicinal plants that possess several bioactive secondary metabolites, such as phenolics, iridoids, triterpenes, and alkaloids. Triterpenoids are the ubiquitous and dynamic secondary metabolites that are deployed by plants for chemical interactions and protection under biotic/abiotic stress. Plantago ovata, a cultivated species, is the source of psyllium, while Plantago major, a wild species, has significant therapeutic potential. Wild species are considered more tolerant to stressful conditions in comparison to their cultivated allies. In view of this, the present study aimed to decipher the terpenoid biosynthetic pathway operative in P. ovata and P. major using a comparative transcriptomics approach. Majority of terpenoid biosynthetic genes were observed as upregulated in P. major including rate limiting genes of MVA (HMGR) and MEP (DXR) pathways and genes (α-AS, BAS, SM, and CYP716) involved in ursolic acid biosynthesis, an important triterpenoid prevalent in Plantago species. The HPLC output further confirmed the higher concentration of ursolic acid in P. major as compared to P. ovata leaf samples, respectively. In addition to terpenoid biosynthesis, KEGG annotation revealed the involvement of differentially expressed unigenes in several metabolic pathways, aminoacyl-tRNA biosynthesis, biosynthesis of antibiotics, and biosynthesis of secondary metabolites. MYB was found as the most abundant transcription factor family in Plantago transcriptome. We have been able to generate valuable information which can help in improving terpenoid production in Plantago. Additionally, the present study has laid a strong foundation for deciphering other important metabolic pathways in Plantago.
... Borges et al. (2019) reinforce, in laboratory studies, the (2012), mentions the anti-inflammatory and aphrodisiac action of this plant. "Transagem", Plantago major L. (Plantaginaceae), a plant widely used in the community for uterus and ovaries infection, urinary infections and it also helps to prevent and fight cancer in this region (SCI); placing the leaves in a container with approximately 150mL of boiling water and letting it stand for about 3 minutes; subsequently strain and ingest up to 3 cups a day; Nazarizadeh et al. (2013) bring to light in their study, the anti-inflammatory and anti-cancerous action of this plant. "Tipi", Petiveria alliaceae L. (Phytolaccaceae), used in the community, to encourage and give energy, also used for joint pain; as follows: put the leaves in hot water for 10 minutes, to calm down just feel the steam (inhalation), for the pains you need to drink the tea; Lateef et al. (2018) state that this plant has potential action, antioxidant, anticoagulant, antibacterial and antifungal, with excellent in vitro results. ...
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The aim of this research is to make a survey of the socio-environmental characteristics and the ethnobotanical study of medicinal plants used in a traditional community in the Brazilian Northeast, Alagoas. The study was made based on visits with the application of a questionnaire with questions related to the socio-economic element and on the diversity of plants used in herbal medicine. The research was made from March/2019 to February/2020, where families and interviewed plant exhibitors were interviewed for botanical identification. The studied community, which were 24 interviewees, was compiled by residents of the Quilombola community from Pau D'arco in Arapiraca city - Alagoas. Residents interviewed, 15 (62.5%) attended between 56 to 80 years, 11 interviewees about 46% were born in the community and 13 (54%) had a fundamentally incomplete nature. At the end, there were mentioned 30 plant species used for phytotherapeutic purposes, from which presents bigger usage as plants against arterial hypertension (Salvia rosmarinus Schleid), diabetes Mellitos (Croton heliotropiifolius Kunth), pain and inflammation (Alternanthera tenella Colla), present the biggest number of species in the community. The species cited are related to numerous medicinal uses, among which there will be predominant associations associated with cardiovascular and inflammatory processes. The tea is the main way of preparing plants. It is perceived that medicinal plants are only widely used by this Quilombola community of and growth of the crops in the backyard are considered a tradition.
Nowadays, diseases of the respiratory system are a common problem for national and global healthcare. The drugs of synthetic and natural origin, intended for the prevention and treatment of these diseases, are available on the pharmaceutical market. The latter are mainly represented by herbal medicinal products, among which mixture herbal products occupy a special place. Pectoral species No. 2 is a multicomponent herbal medicinal products used for diseases of the upper respiratory tract, it includes coltsfoot leaves (40%), plantain leaves (30%), and licorice roots (30%). It is produced in the form of powder and is dispensed in packs and filter sachet. On the basis of information and analytical research, some approaches to the standardization of pectoral species No. 2 are developed in this study. Currently, the infusion is the main dosage form of pectoral species No. 2; water-soluble biologically active compounds are fully extracted by water and are responsible for the manifested pharmacological effects of pectoral species No. 2 (polysaccharides and triterpene compounds). The development of modern regulatory documentation for complex herbal preparations (mixture herbal products) should be carried out taking into account scientifically based data and harmonized requirements. Additional experimental studies are required to substantiate the indicators and standards of identity and good quality of pectoral species No. 2. Modern regulatory documentation for pharmaceutical substances of plant origin and mixture herbal products made of them (including pectoral species No. 2) should include sections that take into account the principle of raw material-to-drug standardization and methods that can be used in research laboratories with various material and technical support.
Objectives Seizures are abnormal discharge of neurons in the brain. Ferulic acid (FA) is a phenolic compound with antioxidant and neuroprotective effects. The present study aimed to investigate the role of the nitrergic system in the anticonvulsant effect of FA in pentylenetetrazol (PTZ)-induced seizures in male mice. Methods 64 male Naval Medical Research Institute (NMRI) mice weighing 25–29 g were randomly divided into eight experimental groups (n=8). FA at doses 5, 10, and 40 mg/kg alone and in combination with L-nitro-arginine methyl ester (L-NAME) (nitric oxide synthase inhibitor) or L-arginine (L-arg) (nitric oxide [NO] precursor) was administrated (intraperitoneal). PTZ was injected (i.v. route) 30 min after drugs administration (1 mL/min). Seizure onset time was recorded and the nitrite levels of prefrontal cortex and serum were determined by the Griess method. Results FA at doses of 10 and 40 mg/kg significantly increased the seizure threshold as well as reduced the serum and brain NO levels in comparison to the saline-received group. Co-administration of the effective dose of FA (10 mg/kg) plus L-arg significantly decreased the seizure threshold in comparison to the effective dose of FA alone. Co-injection of the sub-effective dose of FA (5 mg/kg) with L-NAME significantly increased the seizure threshold as well as significantly decreased the brain NO level in comparison to the sub-effective dose of FA alone. Conclusions We showed that the nitrergic system, partially at least, mediated the anticonvulsant effect of FA in PTZ-induced seizures in mice. We concluded that L-NAME potentiated while L-arg attenuated the anticonvulsant effect of FA.
Background: Since appearance of (SARS-CoV-2) in China, December 2019, the pandemic of this human virus is accelerating its spread in Morocco as well as worldwide. The prevention of this disease by strengthening immunity through medicinal plants remains an essential choice of the Moroccan population. Methods: This work consists of a declarative survey by questionnaire divided into two parts: the first concerns the informant and the second concerns the plants used. Results: Among 267 participants from different regions of Morocco, 67.04% of respondents use medicinal plants to boost their immunity, disinfect the air, or treat respiratory tract infections that may be related to coronavirus. While 23% have little confidence in herbal medicine, especially in the face of Covid-19. Conclusions: The results obtained constitute a valuable source of information to prevent and inhibit the severity of the infection caused by SARS-CoV-2 by strengthening the immune system. The majority of the listed plants are rich in secondary metabolites and essential oils. They are known for their positive biological effects on respiratory functions. These plants may constitute a database for further research to conduct clinical trials to determine the most commonly used plants effectiveness. © 2021, Ilia State University, Institute of Botany, Department of Ethnobotany. All rights reserved.
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In this study, minimum inhibitor concentrations of acetone and ethyl alcohol extracts of Plantago major L. leaves on predetermined bacteria species was determinbed by Macrodilution liquid (tube) method. Both extracts were tested for nine bacteria species (Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Proteus mirabilis, Salmonella enteritidis). Ethyl alcohol extract showed no antibacterial activity against all species (except for E. coli and B. cereus), but acetone extract was effective on selected bacteria species in different concentrations. Gram Pozitif ve Gram Negatif Bakteriler üzerine Plantago major L.'nin Etanol ve Aseton Ekstraktlarının Antibakteriyel Etkisi Özet Çalışmamızda, Plantago major L. yapraklarından elde edilen aseton ve etil alkol ekstraktlarının Makrodilusyon sıvı (tüp) yöntemi ile Minimum inhibitör konsantrasyonları (MİK) tespit edilmiştir. Her iki ekstrakt dokuz farklı bakteri türü (Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Proteus mirabilis, Salmonella enteritidis) üzerinde test edilmiştir. Etil alkol ekstraktının test bakterilerinden E. coli ve B. cereus dışındaki diğer bakterilere karşı antibakteriyel etkinlik göstermediği saptanırken, aseton ekstraktının ise kullanılan bakteri suşlarına karşı farklı konsantrasyonlarda etkinlik gösterdiği görülmüştür.
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The relationship between the structural characteristics of 29 flavonoids and their antiradical activity was studied. The obtained results suggest that the free radical scavenger potential of these polyphenolic compounds closely depends on the particular substitution pattern of free hydroxyl groups on the flavonoid skeleton. The possible mechanism of action of flavonoids lacking B ring OHs as free radical scavengers has been proposed.
In this study, the antimicrobial activities of the ethanolic extracts of Plantago major (Plantaginaceae), Ononis spinosa (Leguminosae), Lythrum salicaria (Lythraceae) and Juglans regia (Juglandaceae), all used in the folk-medicine, were tested against gram-negative (Escherichia coli, Pseudomonas aeruginosa) and gram positive (Staphylococcus aureus, Bacillus subtilis) bacteria as well as yeast-like fungi (Candida albicans, Candida galabrata, Candida krusei) by the agar diffusion method. All plants showed higher antibacterial activity against E. coli and S. aureus. The highest activity against yeast-like fungi were obtained by the extracts of Ononis spinosa and Lythrum salicaria.
Among the various known therapeutic effects of Plantago major (L.), a few recent studies have shown that preparations of the crude extracts of some plant leaves could prevent or regress the growth of certain tumours. In this study, the effect of P. major, an anticancerogenic, against Ehrlich ascites carcinoma in mice was investigated. The animals were separated into five groups: two groups for control (HC, healthy control; TC, tumour control) and three groups for experiments E1 (25 Mg/ml extract given after tumour inoculation), E2 (50 Mg/ml extract given after tumour inoculation) and E3 (75 Mg/ml extract given after tumour inoculation). Ehrlich ascites tumours (1x106 cells) were injected intraperitonally into the mice's of E1, E2, E3 and TC groups. P. major extract was given in three different concentrations for 10 days orally. Following the administration, all animals sacrificed and their intestine and colon tissues taken out then stained with haematoxilen-eosin for pathological investigations. Pathological findings stated out that P. major extract had inhibitory effect against Ehrlich ascites carcinoma. Therefore, our results show that P. major could be proposed as an effective agent for cancer prevention.
Nature always stands as a golden mark to exemplify the outstanding phenomena of symbiosis. In the western world, as the people are becoming aware of the potency and side effect of synthetic drugs, there is an increasing interest in the natural product remedies with a basic approach towards the nature. Throughout the history of mankind, many infectious diseases have been treated with herbals. A number of scientific investigations have highlighted the importance and the contribution of many plant families i.e. Asteraceae, Liliaceae, Apocynaceae, Solanaceae, Caesalpinaceae, Rutaceae, Piperaceae, Sapotaceae used as medicinal plants. Medicinal plants play a vital role for the development of new drugs. The bioactive extract should be standardized on the basis of active compound. The bioactive extract should undergo safety studies. Almost, 70% modern medicines in India are derived from natural products. Medicinal plants play a central role not only as traditional medicines but also as trade commodities, meeting the demand of distant markets. India has a very small share (1.6%) of this ever-growing global market. To compete with the growing market, there is urgency to expeditiously utilize and scientifically validate more medicinally useful plants.