Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81

Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Science (Impact Factor: 33.61). 08/2013; 341(6149):1009-12. DOI: 10.1126/science.1240985
Source: PubMed
ABSTRACT
Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in
the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of
IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and
mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue
experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.

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