The present investigation was carried out on the experimentally-induced mutations with gamma rays on the seeds of Verbesina encelioides Benthem and Hooker. Ionizing radiations altered the mean total seed germination and also temporal patterns of the germination when compared with control. Higher doses were deleterious for most of the seed parameters studied. The possibility of inducing genetic variability in improvement program of this medicinally important weed is envisaged.