Context: Many athletes and non-athletes have tight hamstring muscles that predispose them to injury when they engage in vigorous physical activity. Thermal and mechanical modalities have been used to enhance the effectiveness of muscle stretching, but evidence supporting their benefits is mixed. Deep oscillation is a relatively new modality being used to promote healing of soft tissue injuries; however, the effect of this modality on muscle extensibility is unknown. Objective: To compare the immediate effect of a single 15-minute treatment of ultrasound (US) and deep oscillation (DO) on the extensibility of hamstring muscles.