Gas chromatography-mass spectrometry (GC-MS) analysis of the crude alkaloid extract of Ziziphus mauritiana Lam., grown in Algerian

Journal of medicinal plant research (Impact Factor: 0.88). 05/2013; 7(20):1511. DOI: 10.5897/JMPR13.4467


The identification of the crude alkaloid extract of the Algerian Ziziphus mauritiana Lam. was carried out
using gas chromatography-mass spectrometry (GC-MS) analysis. The identified ten alkaloids seem to
be reported for the first time in this plant species.

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Available from: Azeddine Kabouche, Jun 08, 2014
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    • "In line with α-linolenic acidcontent of methanol extract in the present analysis, in an earlier study, methanol extract of leaves of Nothapodytesnimmoniana also contained considerably high amount (17.40%) of α-linolenic acid (Uma & Balasubramaniam, 2012). Meanwhile, Chebouat et al. (2013), investigated quite different chemical components in crude alkaloidal extract of Z. mauritiana plant. Moreover, in the present analysis, squalene was also detected at a considerable level (12.09-12.83%) in methanol and hexane extracts of the tested leaves. "
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    ABSTRACT: Ziziphus mauritiana L., is a fruit tree well known for its nutritional and medicinal benefits. The aim of the current study was to investigate the chemical composition as well as biological (antioxidant, antimicrobial, antitumor and anticancer) attributes of different solvent extracts from the leaves of Ziziphus mauritiana. It was established by colorimetric method that chloroform extract had greater amount of total phenolics (84.69 ± 0.92 μg GAE/mg of extract), while methanol extract contained higher content of total flavonoids (46.94±1.55 μg QE/mg of extract). Meanwhile, methanol extract exhibited higher DPPH free radical scavenging potential (IC50 = 0.11 mg/mL) and antimicrobial (antibacterial and antifungal) activity among others. Overall, E. coli was noted to be the most resistant microbial strain against all the tested extracts. Chloroform extract showed strongest antitumor (IC50 = 70.74μg/mL) and anticancer activity (IC50 values of 27.78 and 18.32 μg/mL against human cancer cell lines U937 and HCT-116, respectively) and significantly inhibited the viability of these cell lines.According to GC-MS analysis methyl stearate (15.59%), plamitic acid (38.55%) and α-linolenic acid (26.45%) were identified as the major components of methanol, chloroform and hexane extracts, respectively in addition to presence of several other bioactives. The results of this study conclude that Z. mauritiana leaves extract with efficient biological activities can be explored for potential uses as antioxidant, antitumor and anticancer agents for pharmaceutical industry.
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    ABSTRACT: The present study aimed to determine the phytoconstituent compositions of the leaves of four Ziziphus jujuba ecotypes (Choutrana, Mahdia, Mahres and Sfax). The chromatographic peaks of 18 compounds, including nine major fatty acids, five sterols, two triterpene alcohols and two methysterols, were quantified by the capillary gaseous chromatography method. The major fatty acids identified were linolenic (42.04%) and palmitic (23.04%). Unsaturated fatty acids ranged between 53% and 60%. The predominant sterols (mg/100 g) were β-sitosterol (40.36) and stigmasterol (24.18). Cycloartenol (68.55 mg/100 g) and citrostadienol (12.27 mg/100 g) were the major metylsterols. Methylene cycloartanol ranged between 1.2 mg/100 g (Sfax) and 1.5 mg/100 g (Mahdia). Total phenolic content measured by Folin-ciocalteux ranged from 3.97 mg GAE/g to 6.04 mg GAE/g. The predominant flavonoids identified by HPLC were apigenin (6.1 mg/g) and rutin (1.91 mg/g). The fatty acids and flavonoids in the Z. jujuba leaves were responsible for their therapeutic and pharmaceutical effects. This could explain why Tunisian people traditionally use it as medicine to treat several pathologies.
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