Optimization of a bioactive exopolysaccharide production from endophytic Fusarium solani SD5

Microbiology Laboratory, Department of Botany and Forestry, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Carbohydrate Polymers (Impact Factor: 4.07). 09/2013; 97(2):627-34. DOI: 10.1016/j.carbpol.2013.05.039
Source: PubMed


Endophytic fungi were less investigated for exopolysaccharide production. In this study endophytic Fusarium solani SD5 was used for optimization of exopolysaccharide production. One variable at a time method and response surface methodology were employed to explore the optimum medium compositions and fermentation conditions. The organism produced maximum exopolysaccharide after 13.68 days of incubation at 28°C in potato dextrose broth supplemented with (g%/l) glucose, 9.8; yeast extract, 0.69; KCl, 0.05; KH2PO4, 0.05 with medium pH 6.46. Use of 50ml medium in 250ml Erlenmeyer flask gives highest exopolysaccharide production. The organism produced more than two times higher exopolysaccharide (2.276±0.032g/l EPS) at optimized condition compared to pre-optimized condition (0.96±0.021). In vivo toxicity test established nontoxic nature of the EPS (≤400mg EPS/Kg of body weight). The EPS slightly altered intestinal indigenous bacteria and influenced the growth of beneficial Lactobacillus spp.

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    • "In contrast to cell-wall or cytosolic PSs, EPSs do not require drastic methods of extraction with hot water or organic solvents (Madla et al. 2005; Chen et al. 2008a, b; Mahapatra and Banerjee 2013a). Usually, to obtain crude EPS, the culture fluid is precipitated by alcohols such as ethanol (absolute or 95 %) or methanol and only in some cases by acetone or isopropanol at a temperature of 4 °C for a period of 12–24 h (Table 2). "
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    ABSTRACT: Fungal polysaccharides (PSs) are the subject of research in many fields of science and industry. Many properties of PSs have already been confirmed and the list of postulated functions continues to grow. Fungal PSs are classified into different groups according to systematic affinity, structure (linear and branched), sugar composition (homo- and heteropolysaccharides), type of bonds between the monomers (β-(1 → 3), β-(1 → 6), and α-(1 → 3)) and their location in the cell (cell wall PSs, exoPSs, and endoPSs). Exopolysaccharides (EPSs) are most frequently studied fungal PSs but their definition, classification, and origin are still not clear and should be explained. Ascomycota and Basidiomycota fungi producing EPS have different ecological positions (saprotrophic and endophytic, pathogenic or symbiotic-mycorrhizae fungi); therefore, EPSs play different biological functions, for example in the protection against environmental stress factors and in interactions with other organisms. EPSs obtained from Ascomycota and Basidiomycota fungal cultures are known for their antioxidant, immunostimulating, antitumor, and antimicrobial properties. The major objective of the presented review article was to provide a detailed description of the state-of-the-art knowledge of the effectiveness of EPS production by filamentous and yeast Ascomycota and Basidiomycota fungi and techniques of derivation of EPSs, their biochemical characteristics, and biological properties allowing comprehensive analysis as well as indication of similarities and differences between these fungal groups. Understanding the role of EPSs in a variety of processes and their application in food or pharmaceutical industries requires improvement of the techniques of their derivation, purification, and characterization. The detailed analyses of data concerning the derivation and application of Ascomycota and Basidiomycota EPSs can facilitate development and trace the direction of application of these EPSs in different branches of industry, agriculture, and medicine.
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    ABSTRACT: This study examines the production, characterization and bioactivity on plant cell cultured in vitro of exopolysaccharides (EPS) from Syncephalastrum racemosum CBS 443.59. Firstly, the influence of the fungus culture condition in shake flasks (pH, temperature and different carbon and nitrogen sources) on EPS and biomass production was evaluated. In order to enhance EPS production, a new protocol based on two-stage pH fermentation in a 3L stirred fermentor was developed. Under this condition, EPS production increased by 3.55 times, compared to a constant pH process, reaching a maximal EPS concentration of 2.62g/L. Structurally, the EPS contains a polyglucuronic acid backbone, linked essentially with mannose and fucose units and some galactose and glucose units. The bioactivity of EPS as inducer of defence reactions in plant suspension-cultured cells was also studied. Our results show, for first time, that EPS from S. racemosum CBS 443.59 induces, depending on the concentration, PAL activation and H2O2 synthesis in Arabidopsis thaliana cell suspensions.
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    ABSTRACT: In this study, a sulfated polysaccharide with a high molecular weight was isolated from sea cucumber Holothuria nobilis. It is a fucosylated chondroitin sulfate and being named as HOP. We investigated the effects of several processing variables on the oxidative degradation of HOP using fractional factorial design (FFD) and central composite design (CCD). Moreover, the conditions of the hydroxyl free radical-induced degradation were optimized using response surface methodology (RSM). Our data showed that R(H2O2-HOP), reaction pH and H2O2 flow rate could significantly (P < 0.05) affect the degree of hydrolysis (DH) of HOP. The optimum conditions with Fe2+ were found as follows: R(H2O2-HOP) of 0.53; reaction pH of 6.91; H2O2 flow rate of 0.40 mL/min; reaction time of 2 h; reaction temperature of 30 °C; and HOP concentration of 4 mg/mL. Under these optimum conditions, the DH of HOP was 94.173 ± 0.232 (%), which well matched the value (94.152%) predicted by the RSM model. The preliminary structural characterization of o-HOP was analyzed. The results showed that o-HOP consisted of β-D-glucuronic acid, β-D-N-acetyl-galactosamine, α-L-fucose and sulfate groups. The specific rotation of o-HOP was -43.2°. Furthermore, the sulfation patterns of fucose residues in o-HOP were 2,4-O-disulfated fucose, 3-O-sulfated fucose, 4-O-sulfated fucose and non-sulfated fucose, which were consistent with HOP. In addition, we found that the in vitro antitumor activity of the degraded HOP fraction (o-HOP) was higher than that of HOP against human gastric carinoma SGC-7901 cells.
    No preview · Article · Dec 2014 · PROCESS BIOCHEMISTRY
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