Radiation therapy induces the DNA damage response in peripheral blood

Department of Radiation Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Oncotarget (Impact Factor: 6.36). 06/2013; 4(8). DOI: 10.18632/oncotarget.1084
Source: PubMed


Stereotactic body radiation therapy (SBRT) is a radiotherapy modality that delivers highly conformal, ablative doses to a well-defined target. Here, using a semiquantitative multiplexed assay to analyze ATM and H2AX phosphorylation, we show that ATM kinase activity in peripheral blood mononuclear cells is induced following SBRT. This observation of a systemic ATM kinase-dependent DNA damage response in the peripheral blood is unprecedented and promotes the use of ATM serine-1981 phosphorylation as a predictive biomarker for DNA damaging modalities and ATM inhibitors.

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Available from: John C Schmitz, Aug 13, 2014
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