Optical ultra high-speed imaging of ultrasound contrast agents has revealed new detailed information on the dynamics of coated microbubbles, e.g. surface modes and ``compression-only'' behavior. How these non-spherical and non-symmetrical bubble oscillations translate into an acoustic response is unknown. Acoustic studies of individual microbubbles have been hindered by the ability to isolate a single contrast bubble and by the transducer calibration and its corresponding sensitivity. Here we present a combined optical and acoustical setup to characterize individual ultrasound contrast agents. Bubbles were isolated in a capillary fibre by an active flow control. The receiving transducer was accurately calibrated, therefore both the optical and acoustical recordings provide quantitative information on the microbubble response, allowing for a direct comparison between the two methods. For larger bubbles oscillating in the linear regime, the measured acoustic bubble response was in good agreement with the response predicted from the optically recorded radial bubble dynamics.