Efficiency Of The Photodynamic Therapy Using Gold Nanoparticles (np-Au) And PpIX Induced And Not Induced

Article · August 2008with18 Reads
DOI: 10.1063/1.2979296
Abstract
The use of gold nanoparticles (np‐Au) to eliminate cancer has proved to be very effective due to the fact that cancerous cells accumulate it 600% more than healthy cells. In addition they have a high capacity of absorption and dispersion of light. Therefore, the effectiveness of photodynamic therapy (PDT) could be improved by the simultaneous use of np‐Au and photosensitizes (Ps), emphasizing the high efficiency of the PDT to diagnose and to treat pre‐malignant and malignant processes. The aim of this work was to determine the efficiency of PDT using np‐Au and protoporphyrin IX (PpIX) induced and not induced by the δ‐aminolevulinic acid (ALA). It were found the conditions of synthesis of hydrosoluble np‐Au, and were characterized by transmission electronic microscopy (TEM) and UV‐VIS spectroscopy. It was realized a kinetic by TEM to determine the cellular incorporation time of np‐Au, the maximum incorporation of np‐Au was of 16 h. PDT was applied using different doses of np‐Au and photosensitizers. It was observed that the use of PDT simultaneously with np‐Au did not increase the mortality of HeLa cells. In the case of C33, when PpIX not induced is used as photosensitizer simultaneously with np‐Au, the mortality increased 20%.
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