ArticlePDF Available

Abstract

Horses evolved consuming primarily fermentable forage carbohydrates, but forage diets have been traditionally supplemented with grain meals rich in starch and sugar in order to provide additional calories, protein and micronutrients. Starch and sugar are important for performance horses, but the consumption starch-rich meals may cause equine digestive and metabolic disorders. The critical capacity for preileal starch digestibility appears to be 0.35 to 0.4% but may be as little, depending on the source of starch. Small intestinal absorption of simple sugars is limited by the activity and expression of two classes of glucose carrier proteins, which are affected by chronic intake of hydrolyzable carbohydrate but may be sluggish to respond to abrupt changes in diet, further exacerbating the risk of overload. The most rapid fermentation occurs during starch overload or in the presence of fructans. Rapid fermentation perturbs the microbial and pH balance of the cecum and colon, favoring proliferation of Lactobacillus spp and acid production and increasing the risk of colic and laminitis. In addition to digestive disturbances, feeding grain concentrates rich in hydrolyzable carbohydrate may increase the risk of insulin resistance, which has been associated with obesity, laminitis and chronic founder, developmental orthopedic disease, and Cushing's disease in horses. This threshold concentration of starch intake may be a starting point for horse owners, feed manufacturers and veterinarians that may be claimed to be "low" enough to reduce risk in insulin resistant horses sensitive to grain-associated disorders.
Revista Brasileira de Zootecnia
© 2009 Sociedade Brasileira de Zootecnia
ISSN 1516-3598 (impresso)
ISSN 1806-9290 (on-line)
www.sbz.org.br
R. Bras. Zootec., v.38, p.270-276, 2009 (supl. especial)
Correspondências devem ser enviadas para: rhoffman@mtsu.edu
Carbohydrate metabolism and metabolic disorders in horses
Rhonda M. Hoffman
1
1
Middle Tennessee State University, Murfreesboro, TN.
ABSTRACT - Horses evolved consuming primarily fermentable forage carbohydrates, but forage diets have been
traditionally supplemented with grain meals rich in starch and sugar in order to provide additional calories, protein and
micronutrients. Starch and sugar are important for performance horses, but the consumption starch-rich meals may cause
equine digestive and metabolic disorders. The critical capacity for preileal starch digestibility appears to be 0.35 to 0.4% but
may be as little, depending on the source of starch. Small intestinal absorption of simple sugars is limited by the activity and
expression of two classes of glucose carrier proteins, which are affected by chronic intake of hydrolyzable carbohydrate but
may be sluggish to respond to abrupt changes in diet, further exacerbating the risk of overload. The most rapid fermentation
occurs during starch overload or in the presence of fructans. Rapid fermentation perturbs the microbial and pH balance of
the cecum and colon, favoring proliferation of Lactobacillus spp and acid production and increasing the risk of colic and
laminitis. In addition to digestive disturbances, feeding grain concentrates rich in hydrolyzable carbohydrate may increase
the risk of insulin resistance, which has been associated with obesity, laminitis and chronic founder, developmental orthopedic
disease, and Cushing’s disease in horses. This threshold concentration of starch intake may be a starting point for horse owners,
feed manufacturers and veterinarians that may be claimed to be “low” enough to reduce risk in insulin resistant horses sensitive
to grain-associated disorders.
Key Words: carbohydrate metabolism, glucose, horse, insulin resistance
Metabolismo de carboidratos e disfunções metabólicas em equinos
RESUMO - Equinos desenvolvem-se consumindo primordialmente os carboidratos fermentáveis das forragens, porém
as dietas a base de forragens vem sendo suplementadas com dietas a base de grãos, ricas em amido e açúcar, visando fornecer
adicionais calorias, proteínas e micronutrientes. Amido e açucares são importantes para os equinos atletas, porém o consumo
de dietas ricas em amido pode causar problemas digestivos e metabólicos aos equinos. A capacidade critica da digestão pré-ileal
do amido varia entre 0,35 a 0,4%, podendo ser inferior, dependendo da fonte de amido. A absorção de açucares simples, no
intestino delgado, depende da expressão de suas proteínas carreadoras de glicose, as quais são afetadas pela ingestão continua
de carboidratos solúveis, porém podem ser mais vagarosas a responder a mudanças abruptas na dieta prevenindo o risco da
sobrecarga. A fermentação mais rápida ocorre quando amido não digerido no intestino delgado adentra o intestino grosso e
na presença de frutanas. A rápida fermentação perturba o equilíbrio da microbial e do pH de ceco e colon, favorecendo a
proliferação de Lactobacillus spp e produção de ácido lático, aumentando o risco de cólicas e laminite. Adicionalmente aos
distúrbios digestivos, dietas a base de grãos com alta concentração de carboidratos hidrolisáveis, pode aumentar o risco de
resistência a insulina, a qual vem sendo associada com obesidade, laminite, distúrbios crônicos e desenvolvimento de problemas
ortopédicos. O valor mínimo de ingestão de amido pode ser um ponto inicial na dieta de cavalos vencedores, processamento
de alimentos e veterinários que podem estar recomendado a ser baixo o suficiente para reduzir o risco de resistência e insulina
em equinos sensíveis a distúrbios associados a grãos.
Palavras-chave: equinos, glicose, metabolismo de carboidrtatos, resistência a insulina
Introduction
The horse evolved primarily as a grazing and browsing,
hind-gut fermenting herbivore, with a wide range of forage
carbohydrates—hydrolyzable to fermentable—as its main
source of energy. Pastures provide the main habitat and
nutrition for most horses, and the remaining stall-confined
horses have at least one-half of their nutrition supplied by
conserved pasture. Horse owners supplement a diet of
pasture and hay with grain concentrates in order to meet
energy demands of performance and to provide a carrier for
micronutrients that are marginal or deficient in forages.
Common experience has been supported by epidemiological
and experimental studies that associate grain concentrates
271
© 2009Sociedade Brasileira de Zootecnia
Hoffmann
with several digestive and metabolic disorders, including
colic (Clarke et al., 1990; Hudson et al., 2001), laminitis (Pass
et al., 1998), gastric ulcers (Murray, 1994), developmental
orthopedic disease (Kronfeld et al., 1990; Ralston, 1996),
insulin resistance (Hoffman et al., 2003a; Treiber et al., 2005)
and some forms of exertional rhabdomyolysis (Valentine et
al., 2001). The abundant starch in grain concentrates has
been implicated as the culprit, leading to development and
marketing of “low starch” concentrates for horses.
Corresponding trends in human nutrition towards “low
carb diets” have fed wide consumer support of low starch
feeds for horses, perhaps to excess.
While low starch grain concentrates provide an
alternative energy source that is critical for horses with a
history of digestive and metabolic disorders that are sensitive
to starch, these concentrates are not a “one fits all” solution.
Specifically, exercising horses require some dietary starch
in order to appropriately fuel performance. Horses have an
opportunity for small intestinal metabolism of starch and
simple carbohydrates to glucose, which is more
metabolically efficient than hindgut fermentation of fibers
to volatile fatty acids. Compared to fatty acids, glucose (or
its stored form, glycogen) is aerobically metabolized nearly
twice as fast to generate ATP for muscle contraction. As
speed and exertion increase to the point of anaerobic work,
glycogen is metabolically favored over fatty acids.
Carbohydrate digestion
Carbohydrates may be hydrolyzed or fermented in
horses, depending on the linkage of their sugar molecules:
carbohydrates with a-1,4 linked molecules are subject to
enzymatic hydrolysis, while b-1,4 linked molecules must be
fermented. Hydrolyzable carbohydrates include hexoses,
disaccharides, some oligosaccharides (e.g. maltotriose)
and starches not resistant to enzymatic hydrolysis.
Fermentable carbohydrates include soluble fibers (e.g. gums,
mucilages, pectins), some oligosaccharides (e.g. fructans,
galactans), starches resistant to enzymatic hydrolysis,
hemicellulose, cellulose, and lignocellulose.
Hydrolytic digestion
Enzymes secreted in the small intestine specific to
carbohydrate hydrolysis include a-amylase, a-glucosidases
(sucrase, glucoamylase, maltase), and b-galactosidase
(lactase). Relatively little a-amylase is present in equine
saliva, so limited hydrolysis occurs prior to arrival of
carbohydrates in the stomach. In the stomach, gastric acid
hydrolyzes carbohydrates to an extent, independent of
enzymes.
In the small intestine, hydrolysis of carbohydrates is
initiated primarily by pancreatic a-amylase. In the lumenal
phase, a-amylase cleaves a-1,4 linkages but not a-1,6 or
terminal a-1,4 linkages of starch molecules. Amylopectinase
cleaves a-1,6 linkages. The end products of the luminal
phase are disaccharides and oligosaccharides—no free
sugars are yielded. Sucrase, lactase and maltase are
expressed along the length of the equine small intestine at
the brush border mucosal cells (Dyer et al., 2002). Sucrase
activity was higher in the duocenum and jejunum than the
ileum, while maltase activity was similar in duodenum,
jejunum and ileum (Dyer et al., 2002). Functional lactase
was present in all portions of the small intestine of mature
horses, higher in the duodenum and jejunum than the
ileum. Although its activity was lower in mature than
weaned horses, the presence of functional lactase suggests
that mature horses can digest lactose (Dyer et al., 2002).
The action of these disaccharidases at the brush border
mucosal cells completes hydrolysis to yield free sugars,
glucose, galactose and fructose, providing relatively high
energy yield.
Fermentation
Fermentation occurs predominantly in the hind gut of
horses but may occur in any area of the digestive tract
where microorganism populations are sufficiently
established as a result of favorable conditions, such as
adequate retention time and pH greater than 5 (Van Soest,
1994). The presence of viable anaerobic bacteria as well as
acetate, propionate, butyrate and lactate suggests that
limited fermentation occurs in the equine stomach,
particularly in the fundic region and favors lactic acid
(Argenzio et al., 1974; Kern et al., 1974). The brief retention
time in the stomach and the dorsal to ventral pH gradient of
the gastric mucosa likely supports only nominal fermentation
(Murray & Grodinsky, 1989). Some fermentation occurs in
the small intestine of horses (Zentek et al., 1992; Moore-
Colyer et al., 2002), but it is not well known if small intestinal
fermentation occurs independent of large bowel
fermentation or is merely due to reflux of large bowel
contents. Fermentative gases in breath exhalation indicate
that microbial fermentation in the stomach and small intestine
partially degrades starch and fructans, but not pectin and
cellulose (Coenen et al., 2006).
Carbohydrates fermented by intestinal microflora yield
volatile fatty acids, mainly acetate, propionate, butyrate,
and to a lesser extent, lactate and valerate. The relative
proportions of volatile fatty acids produced are dependent
on substrates, i.e. the proportions of dietary forage and
Carbohydrate metabolism and metabolic disorders in horses272
© 2009 Sociedade Brasileira de Zootecnia
concentrate (Longland et al., 1997; de Fombelle et al., 2001;
Hoffman et al., 2001). Increasing proportions of grain
favored production of propionate and lactate at the expense
of acetate (Hintz et al., 1971; Willard et al., 1977; de Fombelle
et al., 2001). Feeding higher percentages of grain depressed
the efficiency of fiber utilization by altering the microbial
ecosystem in the equine cecum and colon (de Fombelle et
al., 2001). Rapid fermentation favors proliferation of
Lactobacilli spp and production of lactate, which is poorly
absorbed (Argenzio et al., 1974; Garner et al., 1978).
Carbohydrate absorption
Two classes of glucose carrier proteins have been
identified in mammalian cells (Shirazi-Beechey, 1995): the
high affinity, low capacity, Na
+
/glucose cotransporter type
I (SGLT1) and facilitative glucose transporters (GLUT). The
SGLT1 is present on the intestinal lumenal membrane and
in kidney proximal tubule absorptive epiethelial cells. It
transports primarily D-glucose and D-galactose across the
brush border membrane against the concentration gradient
by active transport of Na
+
and the Na
+
/K
+
-ATPase (Dyer
et al., 2002). The sugars accumulate within the enterocytes
and are transported down gradient into systemic circulation
via GLUT (Joost & Thorens, 2001). The major site of
glucose absorption in horses is the proximal small intestine,
with glucose transport highest in the duodenum, followed
by jejunum and ileum (Dyer et al., 2002).
The lag time between an abrupt change in dietary
hydrolyzable carbohydrate and the appearance of enhanced
SGLT1 was 12 to 24 h in mice (Ferraris and Diamond, 1993).
Equine SGLT1 has 85% homology with mouse SGLT1 and
92% similarity at the amino acid level (Dyer et al., 2002). In
mice, dietary regulation of glucose transport involves
increased transcription of SGLT1, mainly in crypt cells
(Ferraris and Diamond, 1993). Comparatively in horses,
expression of SGLT1 is regulated at the level of mRNA
abundance (Dyer et al., 2002). The differences in length and
function of horse and mouse digestive tracts may play a role
in appearance of SGLT1 after changes in dietary
hydrolyzable carbohydrate, so direct comparisons should
be considered with caution. If a similar lag time for SGLT1
exists in horse, then in the event of an abrupt change in diet,
sugar transport would be inadequate, thus exacerbating
hydrolyzable carbohydrate overload to the hind gut.
Metabolic disorders in horses associated with
carbohydrates
Carbohydrate overload
Sugars and starches are hydrolyzed in the equine small
intestine up to the point at which the enzymatic capacity
becomes overloaded, and the excess is rapidly fermented in
the hind gut. The critical capacity for starch overload
appears to be in the range of 0.35 to 0.4% of body weight per
feeding (Potter et al., 1992), but may be as little as 0.2%,
depending on the source of starch (Radicke et al., 1991;
Kienzle et al., 1992). Prececal digestion of corn starch
increased from an intake of 0.1% to peak at approximately
0.35% of body weight, then decreased at starch intakes
above 0.4% of body weight (Potter et al., 1992). Similarly,
the presence of ileal starch remained at a plateau from
intakes of 0.1% to approximately 0.25% of body weight
then increased exponentially at intakes above 0.25% of
body weight. Compared to oat starch, feeding corn starch
resulted in lower cecal pH at all levels of starch intake (from
0.1% to 0.4%), and differences in cecal pH between the
starch sources increased in proportion to starch intake
(Radicke et al., 1991). Accumulation of lactic acid may
overpower the buffering mechanism of the hind gut and
lower pH, normally at 6.4 to 6.7 in grazing horses. A cecal
pH of 6 was considered to represent sub-clinical acidosis
(Radicke et al., 1991). A pH less than 6 favors production
of lactic acid (Garner et al., 1978; Van Soest, 1994) and was
associated with clinical conditions such as osmotic
diarrhea, overgrowth of undesired bacterial populations
and lysis of desired bacterial populations, thus increasing
the risk of endotoxemia and laminitis (Sprouse et al., 1987;
Bailey et al., 2002).
Aside from the rapid fermentation of excess
hydrolyzable carbohydrates, other rapidly fermentable
carbohydrates include resistant starches and
oligosaccharides, especially fructans, which may comprise
5 to 50% of the dry matter in cool season grasses (Longland
et al., 1999; Cuddeford, 2001). The b-2,6 glycocidic bonds
in fructans are not hydrolyzed in mammalian small intestine
but may be partially degraded by small intestinal microbes
(Coenen et al., 2006). Fructans were used to initiate equine
carbohydrate overload and laminitis (Pollitt et al., 2003; van
Eps and Pollitt, 2006) and produced a more rapid fall in cecal
pH than an equal amount of corn starch (Bailey et al., 2002).
Insulin resistance
Insulin resistance has been generally defined as a
abnormal metabolic state when normal concentrations of
circulating insulin fail to elicit a normal physiologic response
in target tissues (Kahn, 1978). More specifically, cells in
muscle, adipose tissue and liver that become insulin resistant
require larger concentrations of circulating insulin to
stimulate glucose uptake. In humans, insulin resistance is
273
© 2009Sociedade Brasileira de Zootecnia
Hoffmann
fundamental in the pathology of type II diabetes and is a
risk factor in obesity (Frayn, 2001), cardiovascular disease
and hypertension (Reaven, 1988), polycystic ovaries
(Legro et al., 1998; Legro, 2002), pregnancy loss (Craig et
al., 2002) and colorectal cancer (Kim, 1998; Sturmer et al.,
2006).
Diets rich in simple sugars have been associated with
insulin resistance in several animal and human studies
(Storlien et al., 2000; Bessesen, 2001), and the common
practice feeding starch-rich cereal grains with high glycemic
indices may promote insulin resistance in horses (Hoffman
et al., 2003a; Treiber et al., 2005). Insulin resistance has been
observed in obese (Hoffman et al., 2003a, Frank et al., 2006)
and sedentary (Powell et al., 2002) horses. Similar to
humans, mares became insulin resistant during late
pregnancy and recovered to normal sensitivity during early
lactation (Hoffman et al., 2003b; George et al., 2007). Insulin
resistance may be a risk factor in horses with hyperlipaemia
(Jeffcott & Field, 1985; Jeffcott et al., 1986), osteochondrosis
(Ralston, 1996), Cushing’s disease (Garcia & Beech, 1986;
Johnson, 2003), colic (Hudson et al., 2001), and laminitis
(Pass et al., 1998; Treiber et al., 2006; Hoffman et al., 2007),
especially chronic grass founder (Hoffman et al., 2007).
Dietary therapy alone may not be sufficient to reverse
insulin resistance (Hoffman et al., 2003; Frank et al., 2005).
Exercise is beneficial, as both obese and lean mares had
improved insulin sensitivity after seven days of moderate
exercise training (Powell et al., 2002).
Carbohydrates in horse forages and feeds
Forages
During photosynthesis, green plants produce
glucose and other simple sugars, with oxygen as a
by-product, from water and atmospheric carbon dioxide
in the presence of light:
6 CO
2
+ 12 H
2
O + light energy
C
6
H
12
O
6
+ 6 O
2
+ 6 H
2
O
When the production of sugars exceeds the energy
requirements of the plant, they are converted to storage
carbohydrates, most commonly starch or fructans. Cool
season pasture grasses accumulate fructans, while warm
season grasses and legumes accumulate starch. The
accumulation of storage carbohydrates in plants is affected
by temperature, light intensity and plant growth rate
(Longland et al., 1999; Hoffman et al., 2001). While plants
that accumulate starch are limited to maximum storage when
their chloroplasts are saturated, plants that accumulate
fructans have no self-limiting mechanism, so high
concentrations may accumulate.
Abrupt changes in fructan concentrations were
observed from day to day in rapidly growing pastures and
diurnally as plant composition changed from night to day
or from shade to sunlight (Longland et al., 1999; Longland
& Byrd, 2006; McIntosh et al., 2007). Fructan
concentrations usually rose during the morning, peaked in
the afternoon, and declined to a low overnight until the
early morning hours. Horses grazing in the afternoon, as
compared to morning, may ingest between two to four
times as much fructans (Longland et al., 1999).
An association between an abrupt increase in pasture
plant fructans and the incidence of laminitis has been
suggested. Laminitis has been clinically induced with
3.75 kg of fructan (Pollit et al., 2003; van Eps & Pollit, 2006),
thus establishing a link between pasture fructans and
laminitis. Considering pasture intake and cool season pasture
fructan concetrations, a horse grazing in the summer
potentially could ingest 5 kg or more of fructans per day
(Longland et al., 1999; Longland & Byrd, 2006). Although
the amount of fructans ingested while grazing can be as
much as that used to clinically induce laminitis, it is relevant
to consider that the gradual dose encountered over time
during grazing likely has a far different impact than the
entire dose in a single bolus during clinical induction of
laminitis. Circadian and seasonal patterns in plasma glucose
and insulin in grazing horses have been noted, however,
to correspond with changes in pasture forage sugars,
starches and fructan content (McIntosh et al., 2007a,b).
These changes during periods of pasture growth may
increase the risk of laminitis by exa cerbating insulin
resistance in affected horses.
Glycemic index
The glycemic index is a reflection of plasma glucose and
insulin responses to a meal, an in vivo estimate, rather than
a chemical analysis of the hydrolyzable carbohydrates in a
feed. The glycemic index provides information about the
food but not necessarily the animal. It has been applied
primarily in human nutrition for diabetics in order to formulate
diets with a low glycemic impact, with glycemic index
calculated as a percentage of the response to a standardized
reference: an oral glucose dose or white bread (Jenkins et
al., 1981; Englyst et al., 1996; Wolever and Mehling, 2002).
In horse nutrition, meal-related responses of blood glucose
and insulin to different diets have been quantified in several
reports (Stull & Rodiek, 1988; Rodiek et al., 1991; Pagan et
al., 1999; Williams et al., 2001). Most studies compared
ingestion of different feeds as either equal-weight or
isocaloric meals and did not calculate glycemic index as a
Carbohydrate metabolism and metabolic disorders in horses274
© 2009 Sociedade Brasileira de Zootecnia
percentage of a standardized reference. More recently,
glycemic indices were quantified in a series of studies by
Rodiek (2003) and reported using whole oats as a
standardized reference feed, with the calculated area under
the curve for oats set to a standard value of 100. The range
of feeds tested and their glycemic indices included beet
pulp, 1, alfalfa hay, 26, timothy hay, 32, carrots, 51, oats, 100,
barley, 101, and corn, 117 (Rodiek, 2003). Several factors
may affect glycemic response including meal size, amount
of hydrolyzable carbohydrates in the meals, fiber and fat
content of the feed, processing, intake time, gastric emptying,
digestibility and rate of absorption (Pagan et al., 1999;
Hoekstra et al., 1999).
In human nutrition, the glycemic index provides a
physiological classification of foods useful in developing
nutritional programs for patients with insulin resistance or
non-insulin dependent diabetes. Similarly, glycemic indices
of horse feeds may be useful in developing nutritional
programs for horses with metabolic problems associated
with carbohydrate intake. There is currently a trend in the
horse feed industry to manufacture low or controlled starch
feeds, with claims of reducing the risk of grain-associated
metabolic disorders; however, lack of reports elucidating
the effect of various starch intakes on blood glucose
response leave questions regarding exact concentrations
of dietary starch for horses that may be considered “low.”
A study in this laboratory examined glucose responses in
equal-weight meals that provided intakes of nonstructural
carbohydrate ranging from 0.6 to 2.0 g/kg bodyweight
(Hoffman et al., in press). The magnitude of each glucose
response was calculated as the incremental area under the
curve (AUC) by graphical approximation. The threshold of
glycemic sensitivity, i.e. the inflection point, or knot, after
which higher nonstructural carbohydrate intakes produced
less of a slope in AUC changes, was determined using
nonstructural carbohydrate intake as the independent
variable and blood glucose AUC as the dependent variable.
The results indicated that glucose AUC data have a positive
slope (37.9, r
2
= 0.76) at low nonstructural carbohydrate
intakes, and become more flat (slope = 4.3, r
2
= 0.31) at
higher nonstructural carbohydrate intakes. Thus, dietary
changes in intake at lower nonstructural carbohydrate
concentrations have a greater influence on blood glucose
response compared to dietary changes at higher NSC intakes.
The segmented regression indicated an inflection point at
nonstructural carbohydrate intake equal to 0.3 g/kg BW
(Hoffman et al., in press). These data provide a concentration
of nonstructural carbohydrate intake for horse owners,
feed manufacturers and veterinarians that may be low
enough to be below a threshold at which a glycemic impact
may be noted, and perhaps low enough to thus reduce risk
in horses sensitive to grain-associated disorders.
Literature Cited
ARGENZIO, R.A.; SOUTHWORTH, M.; STEVENS, C.E. Sites of
organic acid production and absorption in the equine
gastrointestinal tract. American Journal of Physiology,
v.226, p.1043-1050, 1974.
BAILEY, S.R.; RYCROFT, A.; ELLIOTT, J. Production of amines
in equine cecal contents in an in vitro model of carbohydrate
overload. Journal of Animal Science, v.80, p.2656-2662,
2002.
BESSESEN, D.H. The role of carbohydrates in insulin resistance.
Journal of Nutrition, v.131, p.2782S-2786S, 2001.
CLARKE, L.L.; ROBERTS, M.C.; ARGENZIO, R.A. Feeding and
digestive problems in horses. Veterinary Clinics of North
America Equine Practice, v.6, p.433-451, 1990.
COENEN, M.; MOSSELER, A.; VERVUERT, I. Fermentative gases
in breath indicate that inulin and starch start to be degraded by
microbial fermentation in the stomach and small intestine of
the horse in contrast to pectin and cellulose. Journal of
Nutrition (Supplement), v.36, p.2108S-2110S, 2006.
CRAIG L.B.; KE, R.W.; KUTTEH, W.H. Increased prevalence of
insulin resistance in women with a history of recurrent pregnancy
loss. Fertility and Sterility, v.78, p.487-490, 2002.
CUDDEFORD, D. Starch digestion in the horse. In: PAGAN, J.D.;
GOER, R.J. (Eds.) Advanced equine nutrition. 2.ed.
Loughborough: Nottingham University Press, 2001. p. 95-103.
de FOMBELLE, A.; JULLIAND, V.; DROGOUL, C. et al. Feeding
and microbial disorders in horses: effects of an abrupt
incorporation of two levels of barley in a hay diet on
microbial profile and activities. Journal of Equine Veterinary
Science, v.21, p.439-445, 2001.
DYER, J.; FERNANDEZ-CASTANO, M.E.; SALMON, K.S. et al.
Molecular characterization of carbohydrate digestion and
absorption in equine small intestine. Equine Veterinary
Journal, v.34, p.349-358, 2002.
ENGLYST, H.N.; VEENSTRA, J.; HUDSON, G.J. Measurement of
rapidly available glucose (RAG) in plant foods: a potential in
vitro predictor of the glycaemic response. British Journal of
Nutrition, v.75, p.327-337, 1996.
FERRARIS, R.P.; DIAMOND, J.M. Crypt/villus site of substrate-
dependent regulation of mouse intestinal glucose transporters.
Proceedings of the National Academies of Science, v.90,
p.5868-5872, 1993.
FRANK N.; ANDREWS, F.M.; ELLIOTT, S.B. et al. Effects of
rice bran oil on plasma lipid concentrations, lipoprotein
composition, and glucose dynamics in mares. Journal of
Animal Science, v.83, p.2509-2518, 2005.
FRANK, N.; ELLIOT, S.B.; BRANDT, L.E. et al. Physical
characteristics, blood hormone concentrations, and plasma lipid
concentrations in obese horses with insulin resistance. Journal
of the American Veterinary Medical Association, v.228,
p.1383-1390, 2006.
FRAYN K.N. Adipose tissue and the insulin resistance syndrome.
Proceedings of the Nutrition Society, v.60, p.375-380,
2001.
GARCIA, M.C.; BEECH, J. Equine intravenous glucose tolerance
test: glucose and insulin responses of healthy horses fed grain
or hay and of horses with pituitary adenoma. American
Journal of Veterinary Research, v.47, p.570-572, 1986.
GARNER, H.E.; MOORE, J.N.; JOHNSON, J.H. et al. Changes in
the caecal flora associated with the onset of laminitis. Equine
Veterinary Journal, v.10, p.249-252, 1978.
275
© 2009Sociedade Brasileira de Zootecnia
Hoffmann
GEORGE, L.A.; STANIAR, W.B.; CUBITT, T.A. et al. Nutritional
effects on insulin sensitivity and glucose dynamics in pregnant
and non-pregnant mares. In: EQUINE SCIENCE SOCIETY
SYMPOSIUM, 20., 2007, Hunt Valley, MD. Proceedings...
Hunt Valley: 2007. p.3-4.
HINTZ, H.F.; ARGENZIO, R.A.; SCHRYVER, H.F. Digestion
coefficients, blood glucose levels and molar percentage of
volatile acids in intestinal fluid of ponies fed varying forage-
grain ratios. Journal of Animal Science, v.33, p.992-995,
1971.
HOEKSTRA, K.E.; NEWMAN, K.; KENNEDY, M.A.P. et al.
Effect of corn processing on glycemic response in horses. In:
PROCEEDINGS EQUINE NUTRITION AND PHYSIOLOGY
SOCIETY, 16., 1999, Raleig. Proceedings...Raleigh: Equine
Nutrition and Physiology Society, 1999.
HOFFMAN, R.M. Carbohydrates in horse nutrition. In:
CONFERENCE ON EQUINE NUTRITION RESEARCH, 2004,
College Station, TX. Proceedings... College Station:
Conference on Equine Nutrition Research, 2004. p. 21-37.
HOFFMAN, R.M.; BOST ON, R.C.; STEFANOVSKI, D. et al.
Obesity and diet affect glucose dynamics and insulin sensitivity
in thoroughbred geldings. Journal of Animal Science, v. 81,
p. 2333-2342, 2003a.
HOFFMAN, R.M.; KRONFELD, D.S.; COOPER, W.L. et al. Glucose
clearance in pregnant mares is affected by diet, pregnancy and
lactation. Journal of Animal Science, v.81, p.1764-1771,
2003b.
HOFFMAN, R.M.; WILSON, J.A.; KRONFELD, D.S. et al.
Hydrolyzable carbohydrates in pasture, hay, and horse feeds:
direct assay and seasonal variation. Journal of Animal
Science, v.79, p.500-506, 2001.
HUDSON, J.M.; COHEN, N.D.; GIBBS, P.G. et al. Feeding practices
association with colic in horses. Journal of the American
Veterinary Medical Association, v.219, p.1419-1425, 2001.
JEFFCOTT, L.B.; FIELD, J.R. Current concepts of hyperlipaemia
in horses and ponies. Veterinary Record, v.116, p.461-466,
1985.
JEFFCOTT, L.B.; FIELD, J.R.; MCLEAN, J.G. et al. Glucose
tolerance and insulin sensitivity in ponies and Standardbred
horses. Equine Veterinary Journal, v.18, p. 97-101, 1986.
JENKINS, D.J.A.; THOMAS., D.M.; WOLEVER, T.M.S. et al.
Glycemic index in foods: a physiological basis for carbohydrate
exchanges. American Journal of Clinical Nutrition, v.34,
p. 362-366, 1981.
JOHNSON, P.J. Peripheral cushingnoid syndrome (‘equine metabolic
syndrome’). In: ROBINSON, N.E. (Ed.) Current therapy in
equine medicine. 5.ed. Philadelphia: W.B. Saunders, 2003.
p.812-816.
JOOST, H.G.; THORENS, B. The extended GLUT-family of sugar/
polyol transport facilitators: nomenclature, sequence
characteristics, and potential function of its novel members.
Molecular and Membrane Biology, v.18, p.247-256, 2001.
KAHN, C.R. Insulin resistance, insulin sensitivity, and insulin
unresponsiveness: a necessary distinction. Metabolism, v.27,
p.1893-1902, 1978.
KERN, D.L.; SLYTER, L.L.; LEFFEL, E.C. et al. Ponies vs. steers:
microbial and chemical characteristics of intestinal ingesta.
Journal of Animal Science, v.38, p.559-564, 1974.
KIENZLE, E.; RADICKE, S.; WILKE, S. et al. Praeileale
stärkeverdauung in abhängigkeit von stärkeart und -zubereitung.
Pferdeheilkunde, v.1, p.103-106, 1992.
KIM Y.I. Diet, lifestyle, and colorectal cancer: is hyperinsulinemia
the missing link? Nutrition Review, v.56, p.275-279, 1998.
KRONFELD, D.S.; MEACHAM, T.N.; DONOGHUE, S. Dietary
aspects of developmental orthopedic disease in young horses.
Veterinary Clinics of North America Equine Practice,
v.6, p. 451-466, 1990.
LEGRO, R.S. Detection of insulin resistance and its treatment in
adolescents with polycystic ovary syndrome. Journal of
Pediatric Endocrinology and Metabolism, v.5, p.1367-
1368, 2002 (suppl.).
LEGRO R.S.; FINEGOOD, D.; DUNAIF, A. A fasting glucose to
insulin ratio is a useful measure of insulin sensitivity in women
with polycystic ovary syndrome. Journal of Clinical
Endocrinology and Metabolism, v.83, p.2694-2698, 1998.
LONGLAND, A.C.; CAIRNS, A.J.; HUMPHREYS, M.O. Seasonal
and diurnal changes in fructan concentration in Lolium perenne:
implications for the grazing management of equines pre-disposed
to laminitis. In: EQUINE NUTRITION AND PHYSIOLOGY
SOCIETY SYMPOSIUM, 16., 1999, Raleigh, NC.
Proceedings... Raleigh: Equine Nutrition and Physiology
Society, 1999. p. 258-259.
LONGLAND, A.C.; BYRD, B.M. Pasture nonstructural
carbohydrates and equine laminitis. Journal of Nutrition
v.136, p.2099S-2012S, 2006 (suppl.).
LONGLAND, A.C.; MOORE-COLYER, M.; HYSLOP, J.J. et al.
Comparison of the in sacco degradation of the non-starch
polysaccharide and neutral detergent fibre fractions of four
sources of dietary fibre by ponies. In: PROCEEDINGS EQUINE
NUTRITION AND PHYSIOLOGY SOCIETY SYMPOSIUM,
15., 1997, Fort Worth, TX. Proceedings… Fort Worth: Equine
Nutrition and Physiology Society, 1997. p. 120-121.
MCINTOSH, B.; KRONFELD, D.; GEOR, R. et al. 2007a. Circadian
and seasonal fluctuations of glucose and insulin concentrations
in grazing horses. In: PROCEEDINGS EQUINE NUTRITION
AND PHYSIOLOGY SOCIETY SYMPOSIUM, 20., 2007, Hunt
Valley, MO. Proceedings… Hunt Valley: Equine Nutrition and
Physiology Society, 2007a. p.100-101.
MCINTOSH, B.; KRONFELD, D.; GEOR, R. et al. Circadian and
seasonal patterns in forage nonstructural carbohydrate content.
In: PROCEEDINGS EQUINE NUTRITION AND
PHYSIOLOGY SOCIETY SYMPOSIUM, 20., 2007, Hunt
Valley, MO Anais… Hunt Valley: Equine Nutrition and
Physiology Society, 2007b. p.102-103.
MOORE-COLYER, M.J.S.; HYSLOP, J.J.; LONGLAND, A.C. et al.
The mobile bag technique as a method for determining the
degradation of four botanically diverse fibrous feedstuffs in the
small intestine and total digestive tract of ponies. British
Journal of Nutrition, v.88, p.729-740, 2002.
MURRAY, M.J. Gastric ulcers in adult horses. Compendium
Continuing Educational Practice, v.16, p.792-797, 1994.
MURRAY, M.J.; GRODINSKY, C. Regional gastric pH measurement
in horses and foals. Equine Veterinary Journal, v.7, p.73-
76, 1989 (suppl.).
PAGAN, J. D.; HARRIS, P.A.; KENNEDY, M.A.P. et al. Feed type
and intake affects glycemic response in thoroughbred horses.
In: EQUINE NUTRITION AND PHYSIOLOGY SOCIETY, 16.,
1999, Raleigh, NC. Proceedings… Raleigh: Equine Nutrition
and Physiology Society, 1999. p.149-150.
PASS, M.A.; POLLITT, S.; POLLITT, C.C. Decreased glucose
metabolism causes separation of hoof lamellae in vitro: a trigger
for laminitis? Equine Veterinary Journal, v.26, p.133-138,
1998.
POLLITT, C.C.L; KYAW-TANNER, M.; FRENCH, K.R. et al.
Equine laminitis. In: AMERICAN ASSOCIATION OF EQUINE
PRACTITIONERS, 49., 2003, New Orleans, LA.
Proceedings… New Orleans: American Association of Equine
Practitioners, 2003.
POTTER, G.D.; ARNOLD, F.F.; HOUSEHOLDER, D.D. et al.
Digestion of starch in the small or large intestine of the equine.
Pferdeheilkunde, v.1, p.107-111, 1992.
POWELL, D.M.; REEDY, S.E.; SESSIONS, S.R. et al. Effect of
short-term exercise training on insulin sensitivity in obese and
lean mares. Equine Veterinary Journal, v.34, p.81-84, 2002
(suppl.).
RADICKE, S.; KIENZLE, E.; MEYER, H. Preileal apparent
digestibility of oats and corn starch and consequences for caecal
metabolism. In: EQUINE NUTRITION AND PHYSIOLOGY
Carbohydrate metabolism and metabolic disorders in horses276
© 2009 Sociedade Brasileira de Zootecnia
SOCIETY, 12., 1991, Calgary, Canada. Proceedings... Calgary:
1991. p.43-48.
RALSTON, S.L. Hyperglycemia/hyperinsulinemia after feeding a
meal of grain to young horses with osteochondritis dissecans
(OCD) lesions. Pferdeheilkunde, v.12, p.320-322, 1996.
REAVEN, G.M. Role of insulin resistance in human disease.
Diabetes, v.37, p.1595-1607, 1988.
RODIEK, A. Glycemic index of practical horse feeds.
Agricultural Research Institute Publication, 2006. #00-2-034-
1B. California Agricultural Technology Institute, California
State Univ., Fresno.
RODIEK, A.; BONVICIN, S.; STULL, C. et al. Glycemic and
endocrine responses to corn or alfalfa fed prior to exercise.
Equine Exercise Physiology, v.3, p.323-330, 1991.
SHIRAZI-BEECHEY, S.P. Molecular biology of intestinal glucose
transport. Nutrition Research Reviews v. 8, p. 27-41, 1995.
SPROUSE, R.F.; GARNER, H.E.; GREEN, E.M. Plasma endotoxin
levels in horses subjected to carbohydrate induced laminitis.
Equine Veterinary Journal, v.9, p.25-28, 1987.
ST ANIAR, W.B. Growth and the somatotropic axis in young
thoroughbreds. 2002. Dissertation. Virginia Polytechnic
Institute and State University, 2002.
ST ORLIEN, L.H.; HIGGINS, J.A.; THOMAS, T.C. et al. Diet
composition and insulin action in animal models. British
Journal of Nutrition, v.83, p.S85-S90, 2000 (suppl.).
STULL, C.L.; RODIEK, A.V. Responses of blood glucose, insulin
and cortisol concentrations to common equine diets. Journal
of Nutrition, v.18, p.206-213, 1988.
STURMER, T.; BURING, J.E.; LEE, I.M. Metabolic abnormalities
and risk for colorectal cancer in the physicians’ health study.
Cancer Epidemiological Biomarkers Preview, v.15,
p.2391-2397, 2006.
TREIBER, K.H.; BOSTON, R.C.; KRONFELD, D.S. et al. Insulin
resistance and compensation in Thoroughbred weanlings adapted
to high-glycemic meals. Journal of Animal Science, v.83,
p.2357-2364, 2005.
TREIBER, K.H.; KRONFELD, D.S.; GEOR, R.J. Insulin resistance
in equids: possible role in laminitis. Journal of Nutrition
(Supplement), v.136, p. 2094S-2098S, 2006.
VALENTINE, B.A.; Van SAUN, R.J.; THOMPSON, K.N. et al.
Role of dietary carbohydrate and fat in horses with equine
polysaccharide storage myopathy. Journal of the American
Veterinary Medical Association, v.219, p.1537-1544, 2001.
Van EPS, A.W.; POLLITT, C.C. Equine laminitis induced with
oligofructose. Equine Veterinary Journal, v.3, p.203-208,
2006.
Van SOEST, P.J. Nutritional ecology of the ruminant. 2.ed.
New York: Cornell University Press, 1994. 476p.
WILLARD, J.G.; WILLARD, J.C.; WOLFRAM, S.A. Effect of diet
on cecal pH and feeding behaviors of horses. Journal of Animal
Science, v.45, p.87-93, 1977.
WILLIAMS, C.A.; KRONFELD, D.S.; STANIAR, W.B. et al. Plasma
glucose and insulin response of thoroughbrede mares fed a meal
high in starch and sugar or fat and fiber. Journal of Animal
Science, v.79, p.2196-2201, 2001.
WOLEVER, T.M.S.; MEHLING, C. High-carbohydrate–low-
glycemic index dietary advice improves glucose disposition
index in subjects with impaired glucose tolerance. British
Journal of Nutrition, v.87, p.477-487, 2002.
ZENTEK, J.; NYARI, A.; MEYER, H. Investigations on
postprandial H
2
- and CH
4
-exhalation in the horse.
Pferdeheilkunde, v.1, p.64-66, 2002.
... A inclusão de óleo na ração dos animais atletas favorece a economia do glicogênio intramuscular em diferentes níveis de exercício, aumenta o tempo para a fadiga e contribui para o bem-estar desse grupo de animais, bem como podem ser utilizados para o tratamento de determinadas enfermidades musculares nos equinos (Ribeiro et al, 2004;Ralston, 2008;Harris e Geor, 2009). A inclusão de óleo acrescida de processos, como o extrusamento nas rações, favorece a digestibilidade pré-cecal, reduz as possíveis modificações na microbiota intestinal e contribui para a saúde do trato digestivo, principalmente dos atletas que são normalmente suplementados com quantidades elevadas de concentrados (Ralston, 2008;Hoffman, 2009). ...
... Diferentes estudos têm demonstrado que a quantidade de gordura no concentrado pode interferir na variação da [GLIC] sanguínea dos animais e também de como o alimento é digerido e absorvido nas diversas porções do aparelho digestivo (Hambleton et al., 1980;Ralston, 2008;Hoffman, 2009;Geelen et al., 2001). No atual experimento não foram observadas variações significativas nas diferentes avaliações da glicose sanguínea (jejum, pico, área sob a curva e a curva) entre os tratamentos, mesmo com os concentrados apresentando diferenças entre a percentagem de EE e na quantidade de carboidratos não-estruturais (CNE). ...
... Diferentes pesquisadores afirmam que uma baixa resposta glicêmica de um alimento rico em CNE, como é o caso do milho em grão não processado, pode produzir efeitos adversos no colo e ceco por modificar o microbioma dessas porções do intestino (Ralston, 2008;Harris e Geor, 2009;Hoffman, 2009;Manso et al, 2015). Esses fatos acima descritos podem ser importantes para a manutenção tanto da saúde do trato digestivo quanto da performance atlética dos cavalos de esporte, os quais normalmente apresentam consumo energético superior a 20 Mcal/animal/dia (NRC, 2007). ...
Article
Full-text available
A performance dos cavalos atletas tem melhorando com a elevação da percentagem do extrato etéreo (EE) nos concentrados. Assim, avaliou-se a suplementação de concentrados com diferentes percentagens de EE sobre biomarcadores metabólicos em cavalos atletas, através de um fatorial com cinco equinos e cincos tratamentos [6,5% EE (T6,5%), 8% EE (T8,0%), 12% EE (T12,0%), 14% EE (T14,0%) e 20% EE (T20,0%)]. Os animais receberam suplementação isocalórica por 15 dias e no 16o dia colheu-se sangue nas fases inicial e jejum e após ½ hora, 1, 2, 3, 4, 5 e 6 horas da ingestão dos alimentos. O sangue total foi utilizado para determinar hematrócrito, glicose, proteína plasmática total, ureia, creatinina, triglicérides e colesterol total. Os resultados demonstraram que ocorreu variação na [glicose] (P<0,05) na fase da colheita de sangue, mas sem diferenças (P>0,05) entre os tratamentos e na interação concentrado x fase. Também não ocorreram diferenças (P>0,05) no pico da [glicose] entre tratamentos e entre área sob a curva. Os demais biomarcadores analisados não sofreram modificação (P>0,05). Concluiu-se que a suplementação isoenergética com concentrados utilizando diferentes percentagens de EE não produzem modificações nos biomarcadores analisados, indicando que variações no EE podem ser utilizados, entre 6,5 e 20%, para equinos atletas de forma similar.
... During digestion in the small bowel, starch is primarily broken down by amylase enzymes, leading to glucose liberation. Extremely large quantities of starch in the diet can result in starch overload; which means that undigested starch passes from the small to the large intestine, which can lead to a decrease in colon and caecal pH, often followed by colic laminitis [9]. ...
... In equines, feeding large amounts of fermentable carbohydrates with subsequent acidosis increases the plasma levels of D-lactate up to 25 mMol/L [13]. According to this mechanism, D-lactate can be considered a clinical marker of the development of laminitis, because it is more specific than its L-isoform and more stable than methylglyoxal [9,15]. ...
Article
Full-text available
Laminitis is one of the most devastating diseases in equine medicine, and although several etiopathogenetic mechanisms have been proposed, few clear answers have been identified to date. Several lines of evidence point towards its underlying pathology as being metabolism-related. In the carbonyl stress pathway, sugars are converted to methylglyoxal (MG)—a highly reactive α-oxoaldehyde, mainly derived during glycolysis in eukaryotic cells from the triose phosphates: D-glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. One common hypothesis is that MG could be synthesized during the digestive process in horses, and excessive levels absorbed into peripheral blood could be delivered to the foot and lead to alterations in the hoof lamellar structure. In the present study, employing an ex vivo experimental design, different concentrations of MG were applied to hoof explants (HE), which were then incubated and maintained in a specific medium for 24 and 48 h. Macroscopic and histological analyses and a separation force test were performed at 24 and 48 h post-MG application. Gene expression levels of matrix metalloproteinase (MMP)-2 and -14 and tissue inhibitor of metalloproteinase (TIMP)-2 were also measured at each time point for all experimental conditions. High concentrations of MG induced macroscopic and histological changes mimicking laminitis. The separation force test revealed that hoof tissue samples incubated for 24 h in a high concentration of MG, or with lower doses but for a longer period (48 h), demonstrated significant weaknesses, and samples were easily separated. All results support that high levels of MG could induce irreversible damage in HEs, mimicking laminitis in an ex vivo model.
... Concentrations of the other biomarkers (URE, CREAT, UrAc, COLE-T, TRIG, calcium, phosphorus, and magnesium) were obtained with the use of commercial kits (Doles Reagent) and semiautomatic biochemistry equipment (Doles D 250; Doles). The area under the curve (AUC) for glucose was calculated according to the method described in the literature [18,19]. The analysts had no knowledge of the experimental group to which the animals had been allocated. ...
... In the dietary treatment, sugar and starch content was reduced to an average of 10 %DMB and lower than 1.1 g/kg/meal. 29 Reducing sugar content in the diet is vital as sugar digestion is limited in horses by a reduced amylase availability 25 which, combined with glucose malabsorption, will increase the hindgut fermentation. This is one of the reasons why colic and other gastrointestinal problems are commonly seen in cases with impaired glucose absorption capacity and abnormal OGTT. 2 3 When sugar and starch are not digested and absorbed completely in the small intestine, they reach the large intestine. ...
Article
Malabsorption syndrome results in impaired nutrient digestion/absorption. Diagnostic tests in horses are focused on reduced carbohydrate absorption demonstrated by abnormal oral glucose tolerance test. However, to determine the definitive diagnosis a biopsy should be performed. The objective was to evaluate the progress of horses believed to be suffering from malabsorption syndrome without other infectious conditions, following institution of appropriate dietary advice. Medical records of 15 horses admitted to the equine hospital of Ghent University (2014–2017) were reviewed. All horses had received corticosteroid treatment and individual dietary advice. All horse owners were contacted in 2017 for a follow-up. Most horses (86 per cent) had tolerated the recommended diets well and gained weight. Owners noticed the greatest improvements at three and six months after starting the diet. Adequate dietary formulation may therefore be a valuable adjunct to medical treatment. However, it may take several months before increases in bodyweight and condition are seen.
... Além disso, promove a morte de bactérias que não resistem a este pH, liberando lipopolissacarídeos bacterianos (LPS) ou endotoxinas, que podem ser absorvidas e promover danos teciduais decorrentes da endotoxemia. Esta condição de acidez intestinal irá também afetar o animal através de uma acidose sistêmica, provocando desconforto, cólicas e laminite (Hoffman, 2009). Outras alterações bioquímicas com a rápida fermentação do amido (ou fructanas) no intestino grosso podem incluir um aumento na produção de monoaminas vasoativas (por exemplo, tiramina, triptamina), endotoxinas e exotoxinas, todos os quais têm sido implicados na patogênese de laminite (Bailey et al., 2002;Botteon et al., 2008). ...
Article
Full-text available
In horses, many diseases are associated with the consumption of diets with high levels of grain or nonstructural carbohydrates. Excess of these foods, often con promotes lactic fermentation and intestinal acidosis, causing colic and laminitis. The addition of alkalizing in the diet does not have the same effect on horses as compared to ruminants. This study aimed to use different alkalizing and associations between them, in an attempt to reverse the process and control of acidosis in the large intestine of horses. 20 animals underwent four treatments with a control, one with Al2(OH)3, Mg(OH)2, and finally a combination thereof. The animals were evaluated for changes in fecal pH and clinical changes resulting from the administered products such as occurrence of constipation or diarrhea, and abdominal discomfort. It is concluded that administration of magnesium hydroxide (Mg (OH)2 at a dose of 20 g / 100 kg body weight and the combination of magnesium hydroxide and aluminum hydroxide 20g Al (OH) 3 + 20 g Mg (OH) 2 / 100 kg live weight, were able to significantly (P<0,05) alter the fecal pH, 24 hours after administration, without digestive disorders or significant abdominal discomfort
... Além disso, promove a morte de bactérias que não resistem a este pH, liberando lipopolissacarídeos bacterianos (LPS) ou endotoxinas, que podem ser absorvidas e promover danos teciduais decorrentes da endotoxemia. Esta condição de acidez intestinal irá também afetar o animal através de uma acidose sistêmica, provocando desconforto, cólicas e laminite (Hoffman, 2009). Outras alterações bioquímicas com a rápida fermentação do amido (ou fructanas) no intestino grosso podem incluir um aumento na produção de monoaminas vasoativas (por exemplo, tiramina, triptamina), endotoxinas e exotoxinas, todos os quais têm sido implicados na patogênese de laminite (Bailey et al., 2002;Botteon et al., 2008). ...
Article
Full-text available
Nos equinos, diversas doenças estão associadas ao consumo de dietas com níveis elevados de grãos ou carboidratos solúveis. O excesso destes alimentos, é comum a ocorrencia de fermentação lactica e acidose intestinal, provocando colicas e laminite. A adição de alcalinizantes pura e simples da dieta não exerce o mesmo efeito sobre cavalos quando comparado com os ruminantes. Este estudo teve como objetivo o uso de alcalinizantes diferentes e associações entre eles, em uma tentativa de reverter o processo e controle da acidose no intestino grosso de cavalos. 20 animais foram submetidos a quatro tratamentos com um controle, outro com Al(OH)3, Mg(OH)2, finalmente, a combinação destes. Os animais foram avaliados quanto a alterações no pH fecal e alterações clínicas decorrentes dos produtos administrados, como ocorencia de constipação ou diarréia, e desconforte abdominal. Conclui-se que a administração de hidroxido de magnésio (Mg (OH)2 na dose de 20g/100 Kg de peso vivo e a associação de hidroxido de magnésio e hidróxido de aluminio 20g Al(OH)3 + 20g Mg(OH)2 /100 Kg de peso vivo, foram capazes de alterar significativamente (P
... However, in horses used in different types of sports, the increased nutritional energy requirement leads to the use of diets high in hydrolyzable carbohydrates originating from the addition of cereal grains [2]. Because equines have reduced production and secretion of pancreatic amylase [3], in addition to a limited ability to transport monosaccharides through the intestinal mucosa [4], excessive quantities of starch (>2 g kg À1 day À1 ) supplied in the diet may escape enzymatic digestion and be fermented in the cecum colon, predisposing the organism to colics, endotoxemia, and laminitis [5]. ...
Article
Maintaining animal health and performance relies on the availability of an appropriate diet. For herbivores, accurate assessment of forage nutrient quality is critical for appropriate diet formulation and rationing, including potential supplementation. Near-Infrared Reflectance Spectroscopy (NIRS) is a rapid method that is used in place of traditional chemical methodologies (wet chemistry) to predict analyte contents in forage samples. The method relies on scanning a sample with near-infrared light and predicting the analyte content by comparing the reflected spectra to a model which has been developed with samples of known analyte content measured by wet chemistry. The purpose of this study was to examine the accuracy of four NIRS-based methods on haylage from seven farm holdings compared with wet chemistry (the control). We analysed 64 samples for a range of analytes (dry matter (DM), pH, ash, acid detergent fibre expressed inclusive of residual ash (ADF), neutral detergent fibre assayed with a heat stable amylase and expressed inclusive of residual ash (aNDF), crude protein and water-soluble carbohydrate (WSC)) commonly assessed for haylage quality in equid nutrition. We compared results obtained by wet chemistry to corresponding NIRS-based predictions from four commercially available NIRS services. The results revealed large discrepancies amongst all five methods. For DM, average bias (mean±SD) for three reported methods was -15.5±188.4, -10.1±50.4, 12.9±33.8 g/kg respectively and for WSC reporting positive bias from four methods of 26.9±51.3, 24.8±38.2, 26.2±50.1 and 14.5±45.2, g/Kg respectively. The extent of these discrepancies from the wet chemistry also varied by analyte where for example, predictions for DM were more reliable than those for WSC and results demonstrated that predictions obtained by NIRS could result in feeding forage outside of target nutritional values.
Article
Full-text available
Atlar, fiziksel aktiviteleri yüksek olmasına rağmen mide yapılarından dolayı düşük enerjili ve selülozca zengin yemlerle beslenmekte ve ortalama 1‐2 saatte bir yem tüketmek zorundadırlar. Fakat atçılık sporunun gelişmesiyle birlikte, enerji ihtiyaçlarının artması, atların nişasta ve şeker içeriği yüksek rasyonlar ile beslenmesinin önemini ön plana çıkarmıştır. Atların, yemlerinde bulunan nişasta ve şeker konsantrasyonunun yükseltilmesi ile insülin direnci, obezite, laminitis ve osteokondrozis gibi sindirimsel ve metabolik rahatsızlıkların görülme insidensi de artmaktadır. Atların tükettikleri yemin nişasta ve şeker içeriğinin tespit edilip, kontrol edilebilmesi amacıyla Glisemik İndeks (Gİ) değeri üzerinde durulmaktadır. Gİ, yemlerin belli bir zaman diliminde, kan glikoz seviyesini artırma potansiyeli baz alınarak, yemlerin standart bir yeme göre sınıflandırılmasında kullanılan ve yüzdelik olarak ifade edilen bir indekstir. Atlarda, Gİ hesaplamasında standart olarak en çok glikoz, yulaf, mısır ve çayır otu kullanılmaktadır. Nişasta ve şekerlerden elde edilen glikozun sindirimini ve emilimini çok sayıda faktör etkilemektedir. Bu faktörlerin bilinmesi, Gİ’nin kullanılabilirliğini daha iyi anlamayı sağlayacaktır. Bu derleme, atlarda Gİ değerlerini etkileyen faktörleri, Gİ’nin yüksekliğinin atlarda meydana getirdiği rahatsızlıklar ve kullanılabilirliği hakkında bilgi vermek amacıyla hazırlanmıştır
Article
Full-text available
The aim of this work was to evaluate the changes of aerobic microbiota in the colon of horses subjected to carbohydrate overload (CO) and to verify the effect of virginiamycin administered at different times on the colonic microbiota. Were used four colostomized horses in a randomized 4x4 crossover design. The animals were subjected to CO and treated with different doses of virginiamycin (V), according to the treatments of V: C (control) = 0 mg, V0=5 mg V at zero hours after CO (ACO); V12 V = 5mg at 12 hours ACO; V0-12 = 5mg of V at zero hours ACO and 5mg of V at 12 hours ACO. The initial microbiota was related to Gram-positive / Gram-negative bacteria of the order of 53% and 46% respectively. Was isolated the gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae, Serratia rubideae, Shigella sonnei, Enterobacter aerogenes, E. agglomerans, E. sakazakii. And the gram-positive bacteria: Bacillus sp., Corynebacterium sp., Pseudomonas sp., Listeria sp., Streptococcus sp., Staphylococcus sp., and Levedures. The administration of virginiamycin simultaneously to CO inhibited the growth of gram-positive bacteria but does not prevent the reduction of pH and death of Gram-negative bacteria. Virginiamycin 12 hours ACO reduced the population of gram-positive and promoted an increase in pH. We conclude that administration of virginiamycin simultaneously with carbohydrate overload or 12 hours later may be beneficial for control of the population of gram-positive bacteria, producing lactic acid.
Conference Paper
Polycystic ovary syndrome (PCOS) appears to be a lifelong disorder. The stigmata of insulin resistance, including obesity and premature adrenarche, may be early forms of its presentation. Girls with premature pubarche, characterized by elevated dehydroepiandrosterone levels and hyperinsulinemia, are at high risk for developing the full PCOS phenotype, including ovarian hyperandrogenism and chronic anovulation. Because PCOS is associated with a 40% prevalence of abnormal glucose tolerance, every adolescent patient should be evaluated regularly for glucose intolerance with a 2-hour oral glucose tolerance test and for lipid abnormalities with a fasting lipid profile. The role of insulin-sensitizing medications such as metformin in adolescents with PCOS is unclear; short-term studies suggest that these agents improve circulating androgens and ovulatory frequency. Primary prevention of diabetes mellitus and cardiovascular disease by lifestyle modification, including regular exercise and a balanced diet, is particularly important in adolescents, who have the opportunity to establish healthy habits before entering adulthood. The findings of diabetes prevention trials suggest that these interventions may be more efficacious than pharmacological therapy.