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Ozone In Medicine

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Abstract

Ozone therapy has been used as a complementary medical approach for half a century but it has encountered skepticism by orthodox medicine because, particularly in the past, it has been used by practitioners and others without a rational basis and appropriate controls. With the advent of modern medical ozone generators incorporating a photometer, it has become possible to obtain precise ozone concentrations and to evaluate some mechanisms of action and possible toxicity. In contrast with the respiratory tract, human blood exposed to appropriate ozone concentrations is able to tame its strong oxidant properties and neither acute, nor chronic side effects have ensued in millions of patients treated with ozonated autohaemotherapy (O3-AHT). This review summarizes our studies aimed at clarifying biological effects, defining any possible damage, the therapeutic window and suitable doses able to express a therapeutic activity. A very interesting and promising aspect is the induction of the so-called heat stress proteins (HSP) leading to adaptation to a chronic oxidative stress. The use of ozone in human therapy has been reviewed but so far very few controlled clinical studies have been reported. Mostly on the basis of anecdotal results, ozone therapy appears useful in infectious diseases, immune depression, vascular disorders, degenerative diseases and orthopedics.
... La generación de ERO y POL ejercen las siguientes acciones biológicas: (4,6,7,8) (4,9) La activación del NrF2/ERA: 1) incrementa los niveles de antioxidantes directos como GSH, monóxido de carbono (CO) y bilirrubina. ...
... S 9) Inhibe la insulina y la producción de hormona de crecimiento, incrementando la resistencia al estrés. (9,10) El 4-HNE también puede atenuar la inflamación mediada por los receptores tipo toll (TLR), cuya activación es esencial para iniciar la respuesta inflamatoria contra los patógenos al "disparar" la producción de citocinas inflamatorias, realzando la inmunidad adaptativa. (11) ...
... La ozonoterapia puede activar al factor nuclear activado de células T (FNAT) el cual también induce la transcripción de citocinas como IL2, FNTα, IL6, IFN γ y elementos de respuesta inmune en el ADN. (3,4,6,9,12) El H 2 O 2 y los lipoperóxidos realzan la activación de los neutrófilos y la actividad fagocítica. De este modo aumenta la producción de H 2 O 2 , muy útil al ser letal para las bacterias y provocar la lisis de su pared celular. ...
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The Ozone-therapy allows using the potential of ozone reactions with body molecules in order to obtain a desired biological effect treating diseases as cancer and infection. The aim of this work is to shortly describe the attributable actions of therapeutic use of medicinal ozone to put it into the treatment schemes. By means of reviewing recent issues about the topic there was compiled the information that permits to conclude that usage of medical ozone, in appropriated dose, is an advantage in treatment of patients in the institute.
... Концентрация кальпротектина в кале увеличивалась на 2-е сут и снижалась на 4-е и 6-е сут, но на всех сроках наблюдения оставалась значимо выше, чем в группе интактных животных. Ранее нами показано, что ректальные инсуффляции озона при ОИК усиливают окислительную деструкцию белков и липидов в очаге повреждения толстой кишки на 2-е сут, что обусловлено прямыми окислительными свойствами озона, его способностью выступать в роли прооксиданта [18,24]. На начальных этапах воспаления озон активирует факторы транскрипции (HIF-1α, NF-kβ) и повышает продукцию провоспалительных цитокинов (IL-1β, IL-6 и TNF-α) [25]. ...
Article
Введение. Уровень маркеров воспаления при воспалительных заболеваниях кишечника (ВЗК) имеет не только диагностическое и прогностическое значение, но важен и для оценки эффективности терапии. Побочные эффекты и рефрактерность базисных средств терапии ВЗК, включая производные 5-аминосалициловой кислоты (5-АСК), явились предпосылкой для оценки эффектов озона - плейотропного фактора регуляции гомеостаза. Цель работы - проведение сравнительного анализа противовоспалительного действия ректального применения озона и 5-АСК при оксазолон-индуцированном колите (ОИК). Методика. На 73 крысах Wistar моделировали ОИК вначале накожным, затем ректальным применением оксазолона. В течение 6 сут применяли ректальные инсуффляции озона в дозе 0,1 мг/кг 1 раз в сутки или ректальные суппозитории с 50 мг 5-АСК 2 раза в сутки. Оценивали выраженность клинических симптомов по индексу активности болезни (DAI), морфологических признаков повреждения кишки - по индексу повреждения тканей (TDI). Определяли концентрацию кальпротектина в кале, содержание в крови лейкоцитов, С-реактивного белка, НСТ-редуцирующую способность нейтрофилов крови. Результаты. При ОИК максимальное увеличение DAI и содержания кальпротектина в кале наблюдается на 6-е сут, НСТ-редуцирующей способности нейтрофилов крови и количества в крови нейтрофилов и моноцитов - на 2-е сут, концентрации С-РБ в сыворотке крови и выраженности морфологических признаков повреждения в толстой кишке - на 4-е и 6-е сут. Применение озона на 4-е и 6-е сут снижает DAI, TDI, содержание кальпротектина в кале и концентрацию С-РБ в сыворотке; на 2-е, 4-е, 6-е сут снижает количество в крови лейкоцитов за счет лимфоцитов, ограничивает кислород-зависимый метаболизм нейтрофилов крови; на 2-е сут увеличивает содержание кальпротектина в кале. Эффекты ректальных инсуффляций озона по сравнению с применением 5-АСК сопоставимы по данным DAI, TDI; менее выражены в отношении уменьшения размера язвенного дефекта на 2-е и 6-е сут, снижения концентрации в кале кальпротектина на 6-е сут, концентрации С-РБ в сыворотке и количества лейкоцитов в крови на 2-е, 4-е, 6-е сут; более выражены в отношении снижения НСТ-редуцирующей способности нейтрофилов крови. Заключение. Полученные результаты об эффективности ректального применения озона при ОИК в отношении клинических признаков, местных и системных проявлений воспалительного процесса в толстой кишке, полной или частичной сопоставимости показателей ответа острой фазы, клинико-морфологических эффектов озона с ректальным применением 5-АСК являются предпосылкой для дальнейшего изучения механизма противовоспалительного действия озона при ВЗК на доклиническом этапе и возможного применения локальной озонотерапии у больных язвенным колитом и болезнью Крона. Introduction. The investigation of inflammatory markers in inflammatory bowel diseases (IBD) is both of diagnostic and prognostic value and important for the evaluation of therapy. Side effects and the refractoriness to basic therapy for IBD, including 5-aminosalicylic acid (5-ASA) derivatives, have warranted evaluating the effects of ozone as a pleiotropic homeostasis-regulating agent. The aim of the study was to comparatively analyze the anti-inflammatory effects of ozone and 5-ASA when administered rectally to treat oxazolone-induced colitis (OIC). Methods. OIC was induced in 73 Wistar rats by cutaneous followed by oral oxazolone. For 6 days, rectal insufflations of ozone were performed at a dose of 0.1 mg/kg once a day, or rectal suppositories containing 50 mg of 5-ASA were administered twice a day. The severity of clinical symptoms was assessed by the disease activity index (DAI). Morphological signs of intestinal damage were assessed by the tissue damage index (TDI). Calprotectin concentration in feces, number of blood leukocytes, C-reactive protein (CRP), and NBT-reducing ability of blood neutrophils were determined. Results. In rats with OIC, the maximum increases in DAI and fecal calprotectin content were observed on the 6th day. The maximum increases in the NBT-reducing ability of blood neutrophils and the number of blood neutrophils and monocytes were observed on the 2nd day. The maximum serum concentration of CRP and the severity of morphological signs of damage in the colon were observed on the 4th and 6th days. The ozone treatment reduced DAI, TDI, calprotectin content in feces, and the serum concentration of CRP on the 4th and 6th days. On the 2nd, 4th, and 6th days, the ozone treatment reduced blood leukocytes at the expense of lymphocytes and limited oxygen-dependent metabolism of blood neutrophils. On the 2nd day of ozone treatment, there was an increased fecal content of calprotectin. The effects of rectal ozone insufflation were comparable with the effect of 5-ASA by DAI and TDI. These effects were less pronounced with regard to the decrease in ulcer size on the 2nd and 6th days, the decrease in the fecal concentration of calprotectin on the 6th day, the serum concentration of CRP, or the number of leukocytes in the blood on the 2nd, 4th, 6th day. The effects were more pronounced in relation to the decrease in the NBT-reducing ability of blood neutrophils. Conclusion. The effectiveness of rectal ozone treatment in OIC in relation to clinical signs, local and systemic manifestations of the inflammatory process in the colon, complete or partial comparability of acute phase response rates, and clinical and morphological effects of ozone with rectal application of 5-ASA warrant further studying the mechanism of the anti-inflammatory effect of ozone in IBD at the preclinical stage and a possible use of local ozone therapy in patients with ulcerative colitis and Crohn’s disease.
... Ранее нами показано, что внутрибрюшинные инсуффляции озона при ОИК усиливают окислительную деструкцию белков и липидов в очаге повреждения толстой кишки на 2-е сутки, что обусловлено прямыми окислительными свойствами озона, его способностью выступать в качестве прооксиданта [13]. На начальных этапах воспаления озон активирует факторы транскрипции, такие как HIF-1α и NF-kβ, повышает продукцию провоспалительных цитокинов IL-1β, IL-6 и TNF-α [14]. ...
... A grande solubilidade do ozônio na água permite sua reação imediata com quaisquer compostos solúveis e biomoléculas presentes nos fluidos, o que é explicado por ser o terceiro agente oxidante mais forte, depois flúor e persulfato. A alta solubilidade e instabilidade do O3 garantem seu consumo total, não acarretando produtos tóxicos ao organismo vivo 3 . ...
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Pain and asthenia are symptoms of difficult control in cancer patients and greatly determine their quality of life. Conventional ozone therapy is used successfully in the symptomatic control of patients with cancer. We believe that the administration of ozone at high doses through IPO3 could provide an additional benefit in the control of refractory symptoms in patients with cancer. We present below a cancer patient with very deteriorated quality of life, successfully treated with IPO3. The patient is a 48-year-old woman with a history of breast cancer who has a disseminated metastatic recurrence that affects bones, skull, liver, lung and peritoneum, with symptomatology refractory to symptomatic medication. Her quality of life was limited by severe asthenia (which kept her bedridden for more than two months) and pain refractory to symptomatic treatment with opiates and corticosteroids. The patient was treated with a cycle of rectal ozone therapy, consisting of a conditioning cycle with 15 sessions of oxygen / ozone rectally, followed by placement of a peritoneal catheter to subsequently perform five IPO3 sessions. At the end of the treatment, all the symptoms of the patient were resolved completely, allowing the withdrawal of all the analgesic medication, returning the patient to a normal life. There were no side effects, and the beneficial effects of this IPO3 cycle were maintained for more than two months. The analysis showed diminution of the tumor and inflammatory markers during the treatment. Ozone therapy at high doses should be considered in cancer patients with severe symptoms and refractory to conventional treatment.
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