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Prophylactic treatment with a special collagen hydrolysate decreases cartilage tissue degeneration in the knee joints
... In this context of intensive research, it was crucial to gain more insight into the principle mode of action mechanisms of BCP. Therefore, its bioavailability and efficacy in different body tissues and cell types of different species have been investigated in vitro and in vivo (Jennings et al., 2001;McAlindon et al., 2011;Oesser, Proksch, & Schunck, 2008;Oesser & Seifert, 2003;Schunck, Schulze, & Oesser, 2007). ...
... The efficacy of orally administered BCP on the development and progression of OA was investigated with a randomised, placebo-controlled study in an inbred mouse strain (STR/ortmice; validated OA animal model). In this in vivo study, administration of specific collagen peptides led to a statistically significant decrease in cartilage tissue degeneration in the knee when compared to the mice of the placebo group (Oesser et al., 2008;Schunck et al., 2007). ...
... A statistically significant increase in the biosynthesis of aggrecan was also shown by RNA expression and an accumulation in the extracellular matrix of chondrocytes. Moreover, in STR/ort mice, an inbred mouse strain that develops osteoarthritis, an early prophylactic collagen peptide treatment had beneficial effects and alleviated pathophysiological changes in the knee joints [75]. ...
The intake of specific collagen peptides (SCPs) has been shown to decrease activity-related knee pain in young, physically active adults. This trial investigated the effect of a 12-week SCP supplementation in a wider age range of healthy men and women over 18 years with functional knee and hip pain during daily activities. A total of 182 participants were randomly assigned to receive either 5 g of specific collagen peptides (CP-G) or a placebo (P-G). Pain at rest and during various daily activities were assessed at baseline and after 12 weeks by a physician and participants using a 10-point numeric rating scale (NRS). The intake of 5 g SCP over 12 weeks significantly reduced pain at rest (p = 0.018) and during walking (p = 0.032) according to the physician’s evaluation. Participants in the CP-G also reported significantly less pain when climbing stairs (p = 0.040) and when kneeling down (p < 0.001) compared to the P-G. Additionally, after 12 weeks, restrictions when squatting were significantly lower in the CP-G compared with the P-G (p = 0.014). The daily intake of 5 g of SCP seems to benefit healthy adults with hip and knee joint discomforts by reducing pain during daily activities.
... Collagen hydrolysate (CH) possesses cartilage healing properties and helps in the Tanbir Ahmad tanbirvet05@rediffmail.com 1 synthesis of bone matrix due to readily available bioactive peptides and amino acids leading to beneficial effects on osteoarthritis, joint disorders and bone. Oral intake of CH retards the development and progression of cartilage damage [4,5]. When ingested, CH is absorbed through gastrointestinal mucosa and gets accumulated in hyaline cartilage [6]. ...
Purpose
This study was conducted to characterize the extract collagen hydrolysate (CH) from low value buffalo skin using enzymes bromelain (B) and papain (P), including their bioactivities.
Methods
Optimum levels of the two enzymes were determined by Sodium dodecyl sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE) and degree of hydrolysis. Levels (30 and 50 units of B/g of skin and 20 and 30 units of P/g of skin) showing maximum degradation of collagen proteins were used to extract CH and the recovered CH were correspondingly referred as B20, B50, P20 and P30, respectively.
Results
The yield of CH from skin for P20, P30, B30 and B50 was 27.38, 26.32, 20.71 and 16.19%, respectively. SDS-PAGE image of the different CH samples showed complete degradation of α- and β- chains of the collagen protein chains revealing only smear bands. 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging ability was highest (94.51%) for B50 sample followed by 90.59% for P30 at the concentration of 10 mg/ml. Amide I peaks of various CHs as revealed by fourier-transform infrared spectroscopy (FTIR) showed greater disruption in the triple helical structure of collagen chains in P30 and B50 than P20 and B30 samples. Antiarthritic study revealed that B30 and B50 samples had 71.07 and 99.47% inhibition, respectively at the concentration of 50 µg/ml.
Conclusion
Thus, it could be concluded that 30 and 50 units of papain and bromelain per gram of skin, respectively, could be used to extract CH from buffalo skin and better utilize this high protein byproduct into high value food supplement.
... In fact, it has been demonstrated that certain peptides from hydrolyzed collagen are absorbed and accumulated in the cartilage [14]. In addition, animal models of OA have obtained promising results in terms of preservation of cartilage structure as a result of long-term ingestion of hydrolyzed collagen [15,16]. Regarding native collagen (both soluble or insoluble), the most studied is type II, which was evaluated initially in rheumatoid arthritis [17] and afterwards in OA [18]. ...
Osteoarthritis (OA) is the most common joint disease, generating pain, disability, and socioeconomic costs worldwide. Currently there are no approved disease-modifying drugs for OA, and safety concerns have been identified with the chronic use of symptomatic drugs. In this context, nutritional supplements and nutraceuticals have emerged as potential alternatives. Among them, collagen is being a focus of particular interest, but under the same term different types of collagens coexist with different structures, compositions, and origins, leading to different properties and potential effects. The aim of this narrative review is to generally describe the main types of collagens currently available in marketplace, focusing on those related to joint health, describing their mechanism of action, preclinical, and clinical evidence. Native and hydrolyzed collagen are the most studied collagen types for joint health. Native collagen has a specific immune-mediated mechanism that requires the recognition of its epitopes to inhibit inflammation and tissue catabolism at articular level. Hydrolyzed collagen may contain biologically active peptides that are able to reach joint tissues and exert chondroprotective effects. Although there are preclinical and clinical studies showing the safety and efficacy of food ingredients containing both types of collagens, available research suggests a clear link between collagen chemical structure and mechanism of action.
... De esta manera la colágena hidrolizada puede ayudar reduciendo los cambios degenerativos de la matriz extracelular articular. 9, 21 Según Oesser et al, el menos cuatro meses, lleva a una disminución pronunciada en la degeneración del tejido cartilaginoso en la articulación de la rodilla y el determinado grado de OA que se presentaba, disminuyó de manera signiretardando la aparición de OA. 26,27 El dolor articular es una de las principales causas de discapacidad en sujetos mayores de 50 años. 11 Existen varios estudios que la OA. 9 OA de rodilla o de cadera, sugieren que la zada reduce el dolor al aumentar la concentración sérica de hidroxiprolina. ...
La osteoartrosis (OA) es la enfermedad reumática de origen multifactorial, más frecuente y antigua del ser humano así como la principal causa de incapacidad y morbilidad en personas mayores de 65 años. Anteriormente el tratamiento de la osteoartritis era sintomático basado en anti-inflamatorios no esteroideos y paracetamol. En los últimos años se han desarrollado nuevas maneras de tratamiento como el uso de la colágena hidrolizada, la cual ha demostrado en múltiples estudios tener efecto sintomático y preventivo al disminuir la inflamación, el dolor y promover la regeneración de cartílago articular, con el fin de actuar como una terapia no solo complementaria sino preventiva. LUX MÉDICA AÑO8, NÚMERO 24, MAYO-AGOSTO 2013, PP 31-36
... Glucosamine has been extensively studied [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] showing positive results in the clinical management of pain and structural improvements [19]. Chondroitin sulfate coupled to Glucosamine, or by itself, has been the center of significant studies [20], but many other compounds synthetic or natural have been investigated, including Vitamin C [21][22][23][24][25]. Particular interest has been paid to Collagen Hydrolysates (CHs) and Fucoidans [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44]. CH is obtained by the enzymatic hydrolysis of collage nous tissues from mammals. ...
... This study was performed to identify the molecular mechanism of the chondroprotective activity of CHs, which has been proposed by other groups [11,52]. Our novel study presents three CH products from fish and pig, which differ significantly with regard to their content of collagen peptides and efficacy. ...
The most frequent disease of the locomotor system is osteoarthritis (OA), which, as a chronic joint disease, might benefit more from nutrition than acute illnesses. Collagen hydrolysates (CHs) are peptidic mixtures that are often used as nutraceuticals for OA. Three CHs were characterized biochemically and pharmacologically. Our biophysical (MALDI-TOF-MS, NMR, AFM) and fluorescence assays revealed marked differences between CHs of fish (Peptan® F 5000, Peptan® F 2000) and porcine (Mobiforte®) origin with respect to the total number of peptides and common peptides between them. Using a novel dual radiolabeling procedure, no CH modulated collagen biosynthesis in human knee cartilage explants. Peptan® F 2000 enhanced the activities of the aggrecanase ADMATS4 and ADMATS5 in vitro without loss of proteoglycan from cartilage explants; the opposite effect was observed with Mobiforte®. Interleukin (IL)-6, matrix metalloproteinase (MMP)-1, -3 and -13 levels were elevated in explants that were treated with Mobiforte® and Peptan® F 5000, but not with Peptan® F 2000. In conclusion, the heterogeneous peptide composition and disparate pharmacological effects between CHs suggest that the effect of a CH preparation cannot be extrapolated to other formulations. Thus, the declaration of a CH as a safe and effective nutraceutical requires a thorough examination of its pleiotropic effects.
... Glucosamine has been extensively studied [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] showing positive results in the clinical management of pain and structural improvements [19]. Chondroitin sulfate coupled to Glucosamine, or by itself, has been the center of significant studies [20], but many other compounds synthetic or natural have been investigated, including Vitamin C [21][22][23][24][25]. Particular interest has been paid to Collagen hydrolysates (CHs) and Fucoidans [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44]. CH is obtained by the enzymatic hydrolysis of collagenous tissues from mammals. ...
... The present study was carried out in order to understand the molecular mechanisms of the possible structure-modifying effect of collagen hydrolysates, as has been insinuated both in an animal model of OA and in a recently published clinical trial [8,38]. Our study is the first to provide an in vitro demonstration of the lack of stimulatory effect of three bovine collagen hydrolysates on the biosynthesis of collagen by human OA knee cartilage. ...
Destruction of articular cartilage is a characteristic feature of osteoarthritis (OA). Collagen hydrolysates are mixtures of collagen peptides and have gained huge public attention as nutriceuticals used for prophylaxis of OA. Here, we evaluated for the first time whether different bovine collagen hydrolysate preparations indeed modulate the metabolism of collagen and proteoglycans from human OA cartilage explants and determined the chemical composition of oligopeptides representing collagen fragments. Using biophysical techniques, like MALDI-TOF-MS, AFM, and NMR, the molecular weight distribution and aggregation behavior of collagen hydrolysates from bovine origin (CH-Alpha®, Peptan™ B 5000, Peptan™ B 2000) were determined. To investigate the metabolism of human femoral OA cartilage, explants were obtained during knee replacement surgery. Collagen synthesis of explants as modulated by 0-10 mg/ml collagen hydrolysates was determined using a novel dual radiolabeling procedure. Proteoglycans, NO, PGE(2), MMP-1, -3, -13, TIMP-1, collagen type II, and cell viability were determined in explant cultures. Groups of data were analyzed using ANOVA and the Friedman test (n = 5-12). The significance was set to p≤0.05. We found that collagen hydrolysates obtained from different sources varied with respect to the width of molecular weight distribution, average molecular weight, and aggregation behavior. None of the collagen hydrolysates tested stimulated the biosynthesis of collagen. Peptan™ B 5000 elevated NO and PGE(2) levels significantly but had no effect on collagen or proteoglycan loss. All collagen hydrolysates tested proved not to be cytotoxic. Together, our data demonstrate for the first time that various collagen hydrolysates differ with respect to their chemical composition of collagen fragments as well as by their pharmacological efficacy on human chondrocytes. Our study underscores the importance that each collagen hydrolysate preparation should first demonstrate its pharmacological potential both in vitro and in vivo before being used for both regenerative medicine and prophylaxis of OA.
... CH induces the novel synthesis of type 2 collagen and proteoglycans in the extracellular matrix in a dose-dependent manner 10 , is readily absorbed across the gastrointestinal mucosa in murine models and distributes to hyaline cartilage, where it accumulates 11 . In the STR/ort mouse model of spontaneous OA, oral administration of CH reduced both the development and progression of cartilage damage 12,13 . A range of clinical reports and observations suggest that CH may benefit symptoms of OA, with one randomized clinical trial producing mixed results 14 . ...
To determine whether either of two magnetic resonance imaging approaches - delayed gadolinium enhanced magnetic resonance imaging of cartilage (dGEMRIC), or T2 mapping - can detect short-term changes in knee hyaline cartilage among individuals taking a formulation of collagen hydrolysate.
Single center, prospective, randomized, placebo-controlled, double-blind, pilot trial of collagen hydrolysate for mild knee osteoarthritis (OA). Participants were allowed to continue the prior analgesic use. The primary outcome was change in dGEMRIC T1 relaxation time in the cartilage regions of interest at the 24-week timepoint. Secondary endpoints included the change in dGEMRIC T1 relaxation time between baseline and 48 weeks, the change in T2 relaxation time at 0, 24 and 48 weeks, the symptom and functional measures obtained at each of the visits, and overall analgesic use.
Among a sample of 30 randomized subjects the dGEMRIC score increased in the medial and lateral tibial regions of interest (median increase of 29 and 41 ms respectively) in participants assigned to collagen hydrolysate but decreased (median decline 37 and 36 ms respectively) in the placebo arm with the changes between the two groups at 24 weeks reaching significance. No other significant changes between the two groups were seen in the other four regions, or in any of the T2 values or in the clinical outcomes.
These preliminary results suggest that the dGEMRIC technique may be able to detect change in proteoglycan content in knee cartilage among individuals taking collagen hydrolysate after 24 weeks.
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