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e396 13th International Congress on Infectious Diseases Abstracts, Poster Presentations
tations, being especially frequent in institutionalized
children.
The aim of the present study was to investigate the
prevalence of intestinal parasitic infections in a Romanian
Children Care Unit. Stool examinations were performed
using the iodine staining for the identification of proto-
zoan cysts and the Willis-Hung method, a special technique
for the identification of helminth eggs. We investigated 80
institutionalized children aged 2 to 8 years. Parasitic infec-
tions were identified in 22 cases (27.5%). Giardia lamblia
(17.5%), Blastocytis hominis (1.2%), Entamoeba coli (3.7%),
Ascaris lumbricoides (5%), Trichuris trichiura (6.2%), Enter-
obius vermicularis (6.2%) and Hymenolepis nana (2.5%) were
diagnosed. We have determined associations of two (27.3%)
and more than two parasites (13.6%) among the children
with intestinal parasitosis.
We have also evaluated 16 adults, members of the med-
ical staff, working in this unit. Parasitic infections were
diagnosed in 6 cases (37.5%). Giardia lamblia (25%), Blas-
tocytis hominis (12.5%) and Trichuris trichiura (12.5%) were
also identified in adults. Association of two parasites was
observed in 33.3% of the positive cases among the members
of the medical staff. Clinical examinations were conducted
to investigate the presence of symptoms in patients. Clinical
signs (diarrhoea, weight loss, abdominal pain and cutaneous
manifestations) were present in children and their medical
staff as well. Our results suggest a possible transmission of
the parasites within this pediatric unit.
doi:10.1016/j.ijid.2008.05.1044
65.057
Investigations of Clinical and Demographical Findings
of Children with Positive Stool Specimen with Non-
pathogenic Intestinal Parasitic Infections
E.C. Dinleyici1,∗, N. Dogan2, S. Reyhanioglu1
1Eskisehir Osmangazi University Faculty of Medicine,
Department of Pediatrics, Eskisehir, Turkey
2Eskisehir Osmangazi University Faculty of Medicine,
Department of Parasitology, Eskisehir, Turkey
Background: Some parasites have been considered non-
pathogenic, but this classification has some limitations
according to recent reports indicating it could be the cause
of different intestinal symptoms and signs. In order to under-
stand the clinical significance of Blastocystis hominis and
other non-pathogenic protozoons, it is important to esti-
mate the characteristics of cases, probably infected with
non-pathogenic intestinal parasites. This aim of this study
was to evaluate clinical characteristics of children with posi-
tive stool-specimen with these intestinal parasites. Material
and Method: In this study, 46 children (22 boys, 24 girls)
aged between 7—203 months (mean 105.0 ±50.4 months)
whose stool examination revealed B. hominis,Dientamoeba
fragilis,Endolimax nana,Cryptosposidium spp., were eval-
uated for clinical symptoms and demographical findings.
For parasitological examination, stool samples prepared by
formol-ethyl acetate concentration and after saline and
iodine preparations for microscopic examination under 10×
and 40×magnification. Also trichrome stained prepara-
tions in ambiguous amoebas cases and modified Erlich Ziehl
Nielsen stained preparations for Cryptosporidium in order
to determinate characteristics of non-pathogenic intestinal
protozoons infections. Children with other bacteriological
and parasitological agents were excluded.
Results: B. hominis was the most common intestinal par-
asite. The frequency rate of intestinal symptoms was 88.4%
in the B. hominis cases and 63% of total group. Abdomi-
nal pain was the most frequent symptom (76.9%). Diarrhea
and distention followed at a rate of 50.0% and 32.6%.
Weight loss and loss of appetite are the following main find-
ings of these children. 39.1% of these patients have been
received treatment and responded favorably to treatment
with metronidazole for 10 days.
Conclusions: Some non-pathogenic parasites are respon-
sible of gastrointestinal symptoms when it is found in high
numbers in the stool and when there no other parasites. It
is important to estimate the characteristics of cases with
B.hominis and other non-pathogenic protozoons for treat-
ment options.
doi:10.1016/j.ijid.2008.05.1045
65.058
American Cutaneuos Leishmaniasis in the Urban Area
from Ilhéus Cit, Bahia State. Characterization of
Autochthones Human Cases and Risk Factors Associated
Y.B. Souza, P.D. Bevilacqua∗
UFV, Vic¸osa, Brazil
Brazil currently confronts the spreading and urbaniza-
tion of American Cutaneous Leishmaniasis (ACL), in big and
middle-sized cities, which is primally zoonotic and related
to forest environments. The present work valued the epi-
demiologic aspects of ACL in Ilhéus, and the possible risk
factors associated to its appearance in the urban area of
this city. A revision of all investigation cards of ACL cases
in Ilhéus, registered by the SVE/SMS/Ilhéus from January
2000 to December 2006 was performed, analyzing the fol-
lows information about the referred cases: age, sex, clinical
forms, diagnostic date and illness evolution. A study on phle-
botomine sand fly occurrence in the urban area of Ilhéus was
carried out from March to August 2006, using CDC light traps.
The canine infection was investigated through indirect flu-
orescent antibody test (IFAT) and BIOGENE enzyme-linked
immunosorbent antibody test (ELISA) for calazar canine.
Additionally, a matched case-control study was carried out
involving the human population. Analysis of investigation
records showed that ACL affects mainly adult subjects, with
cutaneous lesions as the principal clinical form. The season-
ality analysis showed the probable infection period to be
quarter September-November (Spring). No statistically sig-
nificant correlation was observed (p< 0.05) between case
number and climate data (rain fall, humidity and temper-
ature). On the other hands, periods with high rainfall and
humidity showed a smaller number of cases. Lu. Cortelezzii
was the one species collected in urban area. Three seropos-
itive dogs were identified by the immunofluorescence assay.
The case-control study showed that the age group 13 to
44 years (p= 0.0061; OR = 0.13; 95% CI = 0.076—0.22), living
close to woodlands, was associated with decreased risk; the
habit of going to the rural area of Ilhéus showed to be a