Northern blot mRNA expression analysis of 4 genes in 12 patients and 5 control cell lines. The blot was sequentially hybridised with radioactive probes. Using a Storm 820 imaging system (APBiotech, Piscataway, NJ, USA), the expression of four genes was quantified using β-actin expression as normalisation standard. For CMKOR1, MKNK2, MYC and KDM5B the mean expression was 3.67-fold, 1.79-fold, ... [Show full abstract] 2.54-fold and 2.56-fold higher in the patient group as compared to the control group, respectively. Using the Student t test the level of significance was calculated to be 0.00047, 0.0022, 0.000019 and 0.016, respectively.