[Molecular cloning and characterization of Ras-homologues from different developing stages of Echinococcus granulosus].

    Abstract

    The Ras GTPase gene from protoscolex and adult worm of Echinococcus granulosus in Xinjiang were cloned by RT-PCR and named as Eg Ras-pro (GenBank No. EU560397) and Eg Ras-adult (GenBank No. EU560398). Sequence analysis showed that each gene had 552 bp, coding 184 aa with an pI of 6.54. Bioinformatics analysis revealed that Eg Ras-pro and Eg Ras-adult had 98.4% and 98.9% homology to Echinococcus multilocularis Ras and 53.9%-78.8% homology to other species. Phylogenetic analysis showed that Eg Ras-pro and Eg Ras-adult clustered with EmRas and SmRas. The data indicated that EgRas GTPase has been expressed from protoscolex and adult worm of Echinococcus granulosus, and both are highly conservative.