[Simultaneous determination of seven effective component in Houpuwenzhong capsules using high performance liquid chromatography].

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Se pu = Chinese journal of chromatography / Zhongguo hua xue hui 02/2009; 27(1):107-10.
Source: PubMed


A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of seven effective components in Houpuwenzhong capsules. The separation was performed on a Scienhome C18 column (250 mm x 4.6 mm, 5 microm) with methanol-acetonitrile-0.06% phosphonic acid (38 : 27 : 35, v/v/v) as the mobile phase at a flow rate of 1.0 mL/min and 30 degrees C. The detective wavelength was set at 235 nm. There were good linear relationships between the mass concentrations and the peak areas of alpinetin, glycyrrhizic acid, honokiol, cardamonin, costunolide, dehydrocostus lactone and magnolol in the ranges of 0.885 -17.7, 107 - 2140, 8.85 - 17.7, 1.035 - 20.7, 4.85 - 97, 5.9 - 118 and 17.5 - 350 mg/L, respectively. The recoveries were 96.9% - 101.1%, 96.0% - 100.5%, 100.3% - 100.8%, 97.7% - 101.4%, 100.4% - 102.3%, 96.0% - 102.3% and 96.2% - 100.6%, respectively. This method is rapid, simple and suitable for the quality control of Houpuwenzhong capsules.

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    ABSTRACT: Alpinetin is a flavonoidal constituent of seeds of Amomum subulatum Roxb., recently reported to possess vasorelaxant and antiHIV activities. Simple, accurate and precise HPLC and HPTLC methods were developed for the analysis of alpinetin in A. subulatum seed extracts and extraction technique was optimized to get maximum yield using conventional, ultrasonic and matrix solid phase dispersion extraction. HPLC was performed on a C18 column with methanol and water (70:30, v/v) as mobile phase at a flow rate of 1.0 ml/min whereas HPTLC on silica aluminum sheet (60F254) using toluene, dichloromethane and ethyl acetate as solvent system. A sharp peak was obtained for alpinetin at a retention time (Rt) of 5.7 min by HPLC and retardation factor (Rf) of 0.48 by HPTLC. Both methods were validated as per the ICH guidelines and the content of alpinetin was estimated in different extracts. Matrix solid phase dispersion technique was found most suitable for extracting alpinetin as compared to other techniques. Validation data are indicative of good precision and accuracy and proved the reliability of the methods.
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