Article

APOC3 Mutation, Serum Triglyceride Concentrations, and Coronary Heart Disease

Department of Laboratory Medicine & Pathology, University of Minnesota, Minneapolis, MN, USA.
Clinical Chemistry (Impact Factor: 7.91). 06/2009; 55(7):1274-6. DOI: 10.1373/clinchem.2009.124669
Source: PubMed
Download full-text

Full-text

Available from: Jose M Ordovas
  • Source
    • "The most extensively studied is the SstI polymorphism, due to a C→G substitution at nucleotide 3238, in the 3' untranslated region of the gene. Numerous studies have found an association between the presence of a polymorphic SstI site in the untranslated region of the ApoC-III gene with raised ApoC-III and TG concentrations [16-53] and with an increased risk of CAD [53-62]. However, little is known about the interactions of the ApoC-III gene polymorphism and alcohol consumption on serum lipid concentrations. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Both apolipoprotein (Apo) C-III gene polymorphism and alcohol consumption have been associated with increased serum triglyceride (TG) levels, but their interactions on serum TG levels are not well known. The present study was undertaken to detect the interactions of the ApoC-III 3238C>G (rs5128) polymorphism and alcohol consumption on serum TG levels. A total of 516 unrelated nondrinkers and 514 drinkers aged 15-89 were randomly selected from our previous stratified randomized cluster samples. Genotyping of the ApoC-III 3238C>G was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing. Interactions of the ApoC-III 3238C>G genotype and alcohol consumption was assessed by using a cross-product term between genotypes and the aforementioned factor. Serum total cholesterol (TC), TG, high-density lipoprotein cholesterol (HDL-C), ApoA-I and ApoB levels were higher in drinkers than in nondrinkers (P < 0.05-0.001). There was no significant difference in the genotypic and allelic frequencies between the two groups. Serum TG levels in nondrinkers were higher in CG genotype than in CC genotype (P < 0.01). Serum TC, TG, low-density lipoprotein cholesterol (LDL-C) and ApoB levels in drinkers were higher in GG genotype than in CC or CG genotype (P < 0.01 for all). Serum HDL-C levels in drinkers were higher in CG genotype than in CC genotype (P < 0.01). Serum TC, TG, HDL-C and ApoA-I levels in CC genotype, TC, HDL-C, ApoA-I levels and the ratio of ApoA-I to ApoB in CG genotype, and TC, TG, LDL-C, ApoA-I and ApoB levels in GG genotype were higher in drinkers than in nondrinkers (P < 0.05-0.01). But the ratio of ApoA-I to ApoB in GG genotype was lower in drinkers than in nondrinkers (P < 0.01). Multivariate logistic regression analysis showed that the levels of TC, TG and ApoB were correlated with genotype in nondrinkers (P < 0.05 for all). The levels of TC, LDL-C and ApoB were associated with genotype in drinkers (P < 0.01 for all). Serum lipid parameters were also correlated with age, sex, alcohol consumption, cigarette smoking, blood pressure, body weight, and body mass index in both groups. This study suggests that the ApoC-III 3238CG heterozygotes benefited more from alcohol consumption than CC and GG homozygotes in increasing serum levels of HDL-C, ApoA-I, and the ratio of ApoA-I to ApoB, and lowering serum levels of TC and TG.
    Full-text · Article · Aug 2010 · Lipids in Health and Disease
  • [Show abstract] [Hide abstract]
    ABSTRACT: Apolipoprotein (apo) C-III gene polymorphisms have been associated with increased plasma triglycerides (TGs) and coronary artery disease, but the results have not always been concordant among diverse populations. The present study was undertaken to detect the association of the apoC-III 3238C>G polymorphism and several environmental factors with serum lipid profiles in the Guangxi Hei Yi Zhuang and Han populations. A total of 490 subjects of Hei Yi Zhuang and 540 participants of Han Chinese aged 15 to 89 years were randomly selected from our previous stratified randomized cluster samples. Genotyping of the apoC-III 3238C>G was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis and then confirmed by direct sequencing. There was no difference in the genotype and allele frequencies between the 2 ethnic groups (P > 0.05), but G allele and GG genotype frequencies were higher in females than in males, or in high TG than in normal TG individuals in Hei Yi Zhuang (P < 0.01); and in high total cholesterol than in normal total cholesterol subgroups, in high low-density lipoprotein cholesterol (LDL-C) than in normal LDL-C subgroups, or in high apoB than in normal apoB subgroups in Han (P < 0.05). There were also differences in the genotypic frequencies between normal apoA-I and low apoA-I subjects in Hei Yi Zhuang, and between males and females or between normal TG and high TG subgroups in Han (P < 0.05). Serum TG and apoA-I levels were correlated with genotype or allele in Hei Yi Zhuang, and TG, LDL-C, and apoB levels were associated with genotype in Han (P < 0.05). Serum lipid parameters were also correlated with age, sex, alcohol consumption, cigarette smoking, blood pressure, body weight, and body mass index. There were no significant differences in genotypic and allelic frequencies between the Hei Yi Zhuang and Han populations. But the 3238G carriers have unfavorable serum lipid profiles. The differences in the serum lipid profiles between the 2 ethnic groups might result from different gene-environmental interactions.
    No preview · Article · Aug 2010 · Journal of Investigative Medicine
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To investigate whether the increase in tryglyceride levels of our adult population detected formerly was continuing in previous ten years according to data of TEKHARF study cohort. Material and Methods: Fasting plasma trygliceride levels of 2862 participants (mean age 54 years, range between 47-63) were evaluated according to sex and age groups. Tryglyceride levels were measured with enzymatic assays; both original values and logarithmic transformational values were used for analysis. Age standardization was conducted for mean values of age group 30-79 years according to age group intensities of country population. Additionally, ultimate tryglyceride values of 2294 participants were compared to the values 7.8 +/- 2.3 years previously. The correction of mean tryglyceride levels (-2,6 mg/dl for females and +9,8 mg/dl for males) was conducted according to the prediction of an increase related to aging. Results: Triglyceride levels increased to 152.3 mg/dl in males and 135.6 mg/dl in females, -adjusting for age- with an increase of +18.7 mg/dl since 1998. Mean values reached to 177 mg/dl in males and 154 mg/dl for females. Triglyceride levels of participants from urban areas were found to be 7 mg/dl higher compared to the levels of participants from rural areas (p = 0.009). Mean triglyceride levels were found to increase of 25 mg/dl and 20 mg/dl in males and females respectively -after age standardization had been conducted - when compared to screening data of year 1990. Increasing trend was higher in recent period. Conclusion: Triglyceride levels of Turkish adults which was between normal values has been increasing 1 mg/dl and more yearly for both genders after adjusted for age and the rate of this increase appeared to be accelerated in last ten years. This trend is the major indicator underlying the defect of protective proteins setting off the proinflammatory condition/oxidative stress. We conclude that it is the time to campaign in order to reverse this worrisome condition for our community health.
    No preview · Article · Oct 2010 · Turkiye Klinikleri Journal of Medical Sciences
Show more