High expression of WT1 gene in acute myeloid leukemias with more predominant WT1+17AA isoforms at relapse

ArticleinLeukemia research 34(1):46-9 · May 2009with8 Reads
Impact Factor: 2.35 · DOI: 10.1016/j.leukres.2009.04.004 · Source: PubMed

    Abstract

    Real-time quantitative reverse transcriptase polymerase chain reaction method was established for detecting the expression levels of WT1 gene and WT1+17AA isoforms in 226 acute myeloid leukemia (AML) bone marrow (BM) cells. The results showed that WT1 gene was 2-3 logarithms expressed more in AML BM cells at initial diagnosis or relapse than in normal BM cells (p<0.001), with predominant WT1+17AA isoforms expression (the ratio of WT1+17AA/WT1 more than 0.50). Interestingly the ratio of WT1+17AA/WT1 was statistically higher in relapsed AMLs than in initially diagnosed (p=0.01), speculating that WT1+17AA isoforms might participate in AML relapse.