Chen, H. H. et al. Micrococcus endophyticus sp. nov., isolated from surface-sterilized Aquilaria sinensis roots. Int. J. Syst. Evol. Microbiol. 59, 1070-1075

The Key Laboratory for Microbial Resources of the Ministry of Education, PR China.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 06/2009; 59(Pt 5):1070-5. DOI: 10.1099/ijs.0.006296-0
Source: PubMed


A Gram-positive bacterial strain, designated YIM 56238(T), was isolated from plant roots (Aquilaria sinensis), and characterized by using a polyphasic approach. Strain YIM 56238(T) grew optimally at pH 7.0-8.0 and at 28 degrees C. Analysis of the 16S rRNA gene sequence of strain YIM 56238(T) indicated that it belongs to the genus Micrococcus. Chemotaxonomic data strongly supported the classification of this strain within the genus Micrococcus: the cell-wall peptidoglycan contained lysine, glutamic acid, alanine and glycine; the predominant menaquinones were MK-8(H(2)) (63.6 %) and MK-7(H(2)) (21.1 %); the phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown ninhydrin-negative phospholipid; and the major cellular fatty acids were iso-C(15 : 0) (30.95 %) and anteiso-C(15 : 0) (53.75 %). The G+C content of the genomic DNA was 72.9 mol%. A number of physiological features were found that clearly distinguished strain YIM 56238(T) from recognized species of the genus Micrococcus. DNA-DNA hybridization studies suggested that the novel strain represents a separate genomic species. On the basis of the data, therefore, strain YIM 56238(T) represents a novel species of the genus Micrococcus, for which the name Micrococcus endophyticus sp. nov. is proposed. The type strain is YIM 56238(T) (=DSM 17945(T)=KCTC 19156(T)).

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Available from: Wen-Jun Li, Jun 13, 2015
    • "Members of the genus contain A4a and A2a types of peptidoglycan, as defined by Schleifer & Kandler (1972), iso-C 15 : 0 and anteiso-C 15 : 0 as dominant fatty acids and MK-8/MK-8(H 2 ) as the dominant menaquinone . With few exceptions, almost all described species of Micrococcus grow at high salt (10 % and above NaCl) and alkaline pH (pH 10) (Kloos et al., 1974; Liu et al., 2000, 2007; Chen et al., 2009; Zhao et al., 2009; Zhang et al., 2010). Similarly, members of the genus Zhihengliuella have Gram-stain-positive, yellow-pigmented, non-motile, nonendospore-forming , oval to short rod-type cells with high levels of iso-C 15 : 0 and anteiso-C 15 : 0 fatty acids, display the A4a type of peptidoglycan and grow at high concentrations of NaCl (10 % and above) and alkaline pH (pH 10) (Zhang et al., 2007; Tang et al., 2009; Chen et al., 2010; Hamada et al., 2013). "
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    ABSTRACT: Micrococcus lactis MCC 2278T and Zhihengliuella aestuarii JCM 16166T were published in International Journal of Systematic and Evolutionary Microbiology in 2011. They showed high levels of 16S rRNA gene sequence similarity (99.3%). Phylogenetic analysis revealed that M. lactis MCC 2278T and Z. aestuarii JCM 16166T formed monophyletic clade and showed distant relationship with other members of the closely related genera like Micrococcus, Zhihengliuella, Arthrobacter and Citricoccus. Presence of high proportion of iso-C14:0 and iso-C16:0 with low iso-C15:0 distinguished M. lactis MCC 2278T and Z. aestuarii JCM 16166T from other members of the genera Micrococcus and Zhihengliuella. Unlike other members of the genera Zhihengliuella and Micrococcus; M. lactis MCC 2278T and Z. aestuarii JCM 16166T showed growth at low concentrations of NaCl. Thus, based on distinctive phylogenetic, chemotaxonomic and physiological features of these two organisms from other members of the genera Micrococcus and Zhihengliuella, it is clear that they did not fit with existing classification and deserve separate status. DNA-DNA hybridization (DDH) value between the two strains was 63% and indicated that they were considered as separate species. In this study, we propose for the creation of a novel genus "Neomicrococcus" to accommodate them with Neomicrococcus aestuarii gen. nov., comb. nov. (JCM 16166T =KCTC 19557T) as type species. Neomicrococcus lactis comb. nov. (Type strain: MCC 2278T = DSM 23694T) is also proposed.
    No preview · Article · Jul 2015 · International Journal of Systematic and Evolutionary Microbiology
    • "The results of this study indicate that the isolated strain has distinct features from species of the genus Micrococcus with validly published names and that it should be described as a novel species. Besides this study, members of the genus Micrococcus have been detected as endophytes on several other plants including the zea (Johnston-Monje & Raizada, 2011), cotton (Mcinroy & Kloepper, 1995), rice seeds (Kaga et al., 2009) and the roots of Polyspora axillaris (Zhao et al., 2009) and Aquilaria sinensis (Chen et al., 2009). "
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    ABSTRACT: A yellow pigmented Gram- stain-positive, non-motile, non-endospore -forming, spherical-shape endophytic actinobacterium strain AE-6T was isolated from inner fleshy leaf tissues of Aloe barbadensis (Aloe vera) collected from, Pune, Maharashtra, India. Strain AE-6T grew at high salt (10% NaCl), between15 - 41 °C temperature and in 5 -12 pH range. It showed highest (99.6%) 16S rRNA gene sequence similarity with Micrococcus luteus NCTC 2665T and Micrococcocus yunnanensis YIM 65004T followed by Micrococcus endophyticus YIM 56238T (98.8%). Ribosomal protein profiling by MALDI-TOF/MS also showed closest relatedness with M. yunnanensis YIM 65004T and M. luteus NCTC 2665T. Like other members of the genus Micrococcus, strain AE-6T contained high contents of branched chain fatty acids (iso-C15:0 and anteiso-C15:0) and MK-8(H2) & MK-8 as predominant isoprenoid quinones. Cell wall analysis showed "A2 L-Lys-peptide subunit" type of peptidoglycan and ribose as major cell wall sugar. The DNA G+C content was 70 mol%. Results of DNA-DNA hybridization (DDH) with closest relatives from the genus Micrococcus showed less than 70% value. Based on the results of this study, strain AE-6T showed clear-cut delineation from existing members of the genus Micrococcus and proposed as Micrococcus aloeverae sp. nov. with strain AE-6T ( = MCC 2184T =DSM 27472T) as type strain of the species.
    No preview · Article · Jul 2014 · International Journal of Systematic and Evolutionary Microbiology
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    • "Since the first description of the taxon by Cohn in 1872, it has repeatedly been revised (Kokur et al. 1975; Stackebrandt et al. 1995) with the last amended and still valid classification done by Wieser et al. (2002). Accordingly, the ten Micrococcus species are: M. luteus (the type species of the genus, with three biovars; Cohn 1872; Wieser et al. 2002), M. lylae (Kloos et al. 1974; Wieser et al. 2002), M. antarcticus (Liu et al. 2000), M. flavus (Liu et al. 2007), M. endophyticus (Chen et al. 2009), M. yunnanensis (Zhao et al. 2009), M. terreus (Zhang et al. 2010), M. lactis (Chittpurna et al. 2011), M. niistensis (Dastager et al. 2010a), and M. cohnii (Rieser et al. 2012). herbicides, dyes, polycyclic aromatic hydrocarbons, polyacrylonitrile polymers, phthalate esters, and chlorinated biphenyls (Bevinakatti and Ninnekar 1993; Doddamani and Ninnekar 2001; Du et al. 2011; Eaton and Ribbons 1982; El-Sayeda et al. 2005; Fischer-Colbrie et al. 2007; Mulla et al. 2011; Rajee and Patterson 2011; Saratale et al. 2009; Sims et al. 1986; Tallur et al. 2008; Zheng et al. 2009; Zhuang et al. 2003a, b). "
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    ABSTRACT: Micrococci are Gram-positive G + C-rich, nonmotile, nonspore-forming actinomycetous bacteria. Micrococcus comprises ten members, with Micrococcus luteus being the type species. Representatives of the genus play important roles in the biodegradation of xenobiotics, bioremediation processes, production of biotechnologically important enzymes or bioactive compounds, as test strains in biological assays for lysozyme and antibiotics, and as infective agents in immunocompromised humans. The first description of plasmids dates back approximately 28 years, when several extrachromosomal elements ranging in size from 1.5 to 30.2 kb were found in Micrococcus luteus. Up to the present, a number of circular plasmids conferring antibiotic resistance, the ability to degrade aromatic compounds, and osmotolerance are known, as well as cryptic elements with unidentified functions. Here, we review the Micrococcus extrachromosomal traits reported thus far including phages and the only quite recently described large linear extrachromosomal genetic elements, termed linear plasmids, which range in size from 75 kb (pJD12) to 110 kb (pLMA1) and which confer putative advantageous capabilities, such as antibiotic or heavy metal resistances (inferred from sequence analyses and curing experiments). The role of the extrachromosomal elements for the frequently proven ecological and biotechnological versatility of the genus will be addressed as well as their potential for the development and use as genetic tools.
    Full-text · Article · Nov 2012 · Applied Microbiology and Biotechnology
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