Expression of OATP1B3 determines uptake of Gd-EOB-DTPA in hepatocellular carcinomas

Department of Surgery, Graduate School of Medicine, Kyoto University, and Department of Diagnostic Pathology, Kyoto University Hospital, 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan.
Journal of Gastroenterology (Impact Factor: 4.52). 04/2009; 44(7):793-8. DOI: 10.1007/s00535-009-0056-4
Source: PubMed


Gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) is an MRI contrast agent with perfusion and hepatoselective properties. The purpose of the study was to examine uptake of Gd-EOB-DTPA in the hepatobiliary phase in hepatocellular carcinoma (HCC).
A retrospective analysis of 22 patients with HCC who underwent preoperative Gd-EOB-DTPA-enhanced MRI was performed. Enhancement ratios (ERs) and expression levels of the organic anion transporter (OATP) 1B3 protein were examined.
Gd-EOB-DTPA accumulated in the hepatobiliary phase in 6 of the 22 cases. All 6 Gd-EOB-DTPA-positive cases were moderately differentiated HCC, but 11 other moderately differentiated HCCs did not show Gd-EOB-DTPA uptake. Histopathologically, 4 Gd-EOB-DTPA-positive HCCs and 5 Gd-EOB-DTPA-negative HCCs produced bile. HCCs with Gd-EOB-DTPA uptake overexpressed OATP1B3 compared with HCCs without Gd-EOB-DTPA uptake, and OATP1B3 levels were significantly correlated with ERs (r=0.91, P<0.0001).
Uptake of Gd-EOB-DTPA in HCC is determined by expression of OATP1B3 rather than by tumor differentiation or bile production.

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Available from: Koji Kitamura
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    • "It has been suggested that signal-intensity patterns on HP images of hypervascular, progressed HCC depend on the expression of OATP1B3 [28, 29] and may be related to the expression pattern of multidrug resistance protein (MRP) 2 within the nodules [30]. However, it is still unknown whether this theory can be also extended to the evaluation of early HCC, as there has been a lack of data directly comparing the signal intensity on HP images with OATP1B3 expression in early HCC. "
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