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Cyclodextrin Based Spectral Changes

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Chapter 19
Cyclodextrin Based Spectral Changes
Lida Khalafi and Mohammad Rafiee
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http://dx.doi.org/10.5772/52824
1. Introduction
1.1. Cyclodextrins
A cyclodextrin (CyD) is a cyclic oligomer of α-D-glucose formed by the action of certain
enzymes, Bacillus amylobacter, on starch. The first reported reference to a cyclodextrin
was published by Villiers in 1891 [1]. Three cyclodextrins are readily available: α-CyD, β-
CyD and γ-CyD having six, seven and eight glucose units respectively. They are com‐
monly referred to as the native CyDs. For a long time, only the three parent CyDs were
known, but during the past decade many covalently modified CyDs have been prepared
from the native forms [2].
The glucose units are connected through glycosidic α-1,4 bonds. As a consequence of the 4C1
conformation of the glucopyranose units, all secondary hydroxyl groups are situated on one
of the two edges of the ring, whereas all the primary ones are placed on the other edge. The
ring, in reality, is a conical cylinder, which is frequently characterized as a doughnut or wreath-
shaped truncated cone. It is, of course, the possession of this cavity that makes the CyDs
attractive subjects for study. The most notable feature of cyclodextrins is their ability to form
inclusion complexes (host–guest complexes) with a very wide range of solid, liquid and
gaseous compounds. Complex formation is a dimensional fit between host cavity and guest
molecule [3]. This phenomenon bears the name molecular recognition [4].
1.2. Inclusion complex formation
The lipophilic cavity of cyclodextrin molecules provides a microenvironment into which
appropriately sized non-polar moieties can enter to form inclusion complexes [5]. No covalent
bonds are broken or formed during formation of the inclusion complex [6]. The first driving
force of complex formation is release of enthalpy-rich water molecules from the cavity. The
second critical factor is the thermodynamic interactions between the different components of
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the system (cyclodextrin, guest, solvent). The cavity size of the toroidally shaped CyDs and
the structural confrmation and size of the guest molecule are the other parameters that mostly
affect the formation of a guest-CyD complex [2]. As the results of this inclusion, changes of the
chemical or physical properties of both host and guest molecules are generally observed;
opening a wide field of applications in many areas and allowing one to monitor the process
by several experimental techniques [2,7-9].
Figure 1. Structure of α-CyD, β-CyD and γ-CyD
2. Results
2.1. Cyclodextrin based spectral changes
As the result of inclusion complexes formation, the guest molecule is surrounded by the
hydrophobic microenvironment of the CyD cavity. This environmental changes cause to some
considerable changes in chemical properties of guest molecule such as equilibria and kinetic
parameters and some changes in physical properties such as absorption coefficient or quantum
yield, these changes strongly depend on the difference between CyD cavity and the outer
medium.
Spectroscopic techniques are the most frequent ones which have been used for the study of
these changes. Although it should be noted that the phase-solubility is one of the simplest
techniques which have been used other than spectroscopy [10].
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Some of the spectroscopic techniques such as UV-Visible, fluorescence, and NMR spectroscopy
are compatible for the spectral study of the complexes that obtained in solution [11]. But the
infrared spectroscopy, X-ray diffraction, scanning electron microscopy techniques [12,13] and
differential scanning calorimetry [14], are suitable for the inclusion compounds that obtained
in the solid state.
Figure 2. NMR spectra of the trans-1,4-bis[(4-pyridyl)ethenyl]benzene ( BPEB) bridged ligand as function of time for
the self-assembling {[Fe(CN5)]2(BPEB.β-CyD)}6– rotaxane, upon addition of 2 equivalents of β-CyD to the dimer in D2O:
(a) 0 min, (b) 5 min, (c) 30 min, (d) 60 min and (e) 24 hours.
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Among the above techniques some of them such as X-ray diffraction and NMR are prop‐
er for obtaining qualitative information about the inclusion complex. For example 1H
NMR spectra can give us some information about the host to guest mole ratios and sta‐
bility constant and even the orientation of the guest in the host cavity in solution which
no other technique can give.
This section provides a condensed overview of the quantitative applications of host-guest
interactions and molecular recognition which are well-matched with more quantitative
techniques such as UV-Vis absorption and fluorescence.
2.2. UV. Vis. Spectral changes
In spite of the small effects encountered in absorption, peak shifts of the order of a few nm and
changes of the absorption coefficients less than ten percent, UV-Vis spectrometry is an easily
performed first test of the occurrence of complexation in particular in nonfluorescing systems.
Moreover, the power of modern chemometric techniques allows valuable analytical applica‐
tions of small effects of CyD inclusion on UV-Vis spectra. The emphasis of absorption changes
and absorption studies will be on the apparent changes in the chemical properties of guest
molecules, such as acid–base equilibrium. The most distinguished work in this field is report
by Taguchi [15]. He has demonstrated that upon the binding of phenolphthalein to β-CyD
cavity in aqueous solution at pH 10.5, the red-colored dianion form is rapidly transformed into
a colorless lactonoid form.
Figure 3. Proposed mechanism for the colour change of phenolphetalein in the presence of β-CyD.
This effect and some other similar spectral changes may reflect the altered polarity of the cavity
microenvironment and preferential or specific guest–host interactions and stabilization of the
preferred form and suppression of the other form in equilibrium. This is not a comprehensive
review but is mainly intended to provide illustrative examples.
The absorption spectrum of mycophenolate mofetil (MMF) at mild acidic solutions shows an
absorption band which has an absorption maximum at 302 nm for its acidic form (HMF). With
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the increasing of pH, the absorption at 302 nm gradually decreased whereas the absorption
with the 340 nm maximum, for the basic (MF) form, increased, Fig.4. These spectral changes
and presence of an isobestic point indicate the presence of acid base equilibrium for this
immunosuppressant drug.
The spectra of MMF in the presence of varying amounts of β-CyD at constant pH that both
acidic and basic forms are presented in solution are shown in Fig. 5. The spectral change by
increasing the β-CyD concentrations at constant pH is similar to the decreasing the pH of
aqueous MMF solution. These spectral changes indicate suppression of the basic form and
dominance of acidic form in the presence of β-CyD cavity.
Figure 4. The absorption spectra for 4.0×10−4 mol L-1MMF at various pH values. The pH values are (a) 5.0, (b) 6.5, (c)
7.0, (d) 7.5, (e) 8.0, (f) 8.5, (g) 9.0 and (h) 9.5. [Reprinted from Khalafi L, Rafiee M, Mahdiun F, Sedaghat S. / Spectro‐
chim. Acta Part A., 2012; 90 45-49 with permission from Elsevier Science.]
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Figure 5. The absorption spectra for 4.0×10−4 mol L-1MMF in the presence of different concentrations of β-CyD at pH
8.0. The concentrations of β-CyD are: (a) 0.0, (b) 1.0×10-3, (c) 2.0×10-3, (d) 4.0×10-3and (e) 8.0×10-3 M. [Reprinted from
Khalafi L, Rafiee M, Mahdiun F, Sedaghat S. / Spectrochim. Acta Part A., 2012; 90 45-49 with permission from Elsevier
Science.]
Rank Annihilation Factor Analysis (RAFA) is used as an efficient chemometrics algorithm for
the analysis of spectrophotometric data and the conditional acidity constant of MMF and the
stability constant of its acidic and basic forms were obtained in the absence and presence of
β-CyD. Based on these results with increasing β-CyD concentration the acidic form stabilized
and the equilibrium of the system driving to produce acidic form. Consequently the condi‐
tional acidity constant decrease with increasing the β-CyD concentration [16]. The spectro‐
photometric study of neutral red and 4-nitrophenol in the presence of β-CyD are the other
examples of spectral changes with different preferential complexation.
In the case of neutral red the increase in the acidity constants as a function of β-CyD is indicative
of more stabilization of basic (neutral) form rather than positively charged acidic form.
Whereas the study of acid-base equilibrium of 4-nitrophenol show that 4-nitrophenolate (the
negatively charged basic form) has more affinity than the acidic (neutral) form. It has been
claimed that the driving force of more stable inclusion complex of 4-nitrophenolate with β-
CyD is the hydrogen bonding [17, 18].
The above results and some other comprehensive studies show the effect of interaction of guest
molecules with microenvironment of β-CyD cavity. The CyD nanocavity has the characters
similar to an 80% dioxane/water solution and provides a slightly alkaline environment [19].
There are four possible interactions including; hydrophobic, hydrogen binding, Van der Waals
forces and donor-acceptor for the cavity that affect the favored interaction, equilibrium shift
and spectral changes in the presence of β-CyD [20-22].
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2.3. UV. Vis. based Molecular recognition:
The spectral change of an indicator may not be important in molecular recognition itself, but
there is an important concept named as “indicator displacement assay” and/or “spectral
displacement” which have been developed considering theses spectral changes. Spectral
displacement method involves the color changes upon addition of competitive guest mole‐
cules; the dye moiety was excluded from the CyD cavity and located in the aqueous media. In
that state, by environmental changes around the dye moiety, the dye moiety shows its normal
color changes resulting from pH changes [23].
Figure 6. p-Methyl red appended β-CyD chemical sensor
A spectroscopic displacement method is used to determine association constants or the
concentrations of the compounds that are spectroscopically transparent. Each application may
be divided into two classes, the first one is based on competitive inclusion of guest and
indicator in the solution, and the second one is the competition of dissolved guest with the
CyD bonded indicators.
The success of the visible spectral displacement technique involving methyl red, in bonded form,
as the competing reagent applied for the construction of molecular sensor for adamanetanccar‐
boxylic acid, adamantanol, borneol, cyclaxtanol, cyclohexanol and same structures [24, 25].
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The spectrophotometric technique involving phenolphthalein as the competing reagent
appears to be the most promising one. It is based on the fact that in alkaline solutions a
colourless 1:1 complex is formed between phenolphthalein and β-CyD that the red phenolph‐
thalein dianion is partially displaced by a competing reagent to an extent depending upon its
affinity to form a complex with the CyD host. Phenolphthalein-modified β-CyD was synthe‐
sized for the purpose of developing a new type of guest-responsive color change indicator and
the guest-induced absorption changes were used for molecule sensing [26, 27].
Figure 7. Absorption spectra for 4.8×10-5 mol L-1 phenolphthalein in the presence (a) 0.0, (b) 1.0×10-4, (c) 2×10-4, (d)
4×10-4, (e) 7×10-4, and (f) 1.0×10-3 mol L-1 of β -CyD at pH 10.5. [Reprinted from Afkhami A, Madrakian T, Khalafi L. /
Anal. Lett, 2007; 40 2317-2328 with permission from Taylor & Francis.]
Several attempts have been also made on color changes based on competitive complexa‐
tion of some important chemicals with phenolphthalein-CyD inclusion complex. These
chemical sensors are relatively inexpensive, rapid and simple for determination of de‐
sired compounds, such as pharmaceuticals, surfactants and fatty acids which are trans‐
parent in the visible range [28-34]. The sensing abilities of for various guests are roughly
parallel to the binding constants. Fig. 8 shows that by addition of ibuprofen to the phe‐
nolphthalein-β-CyD complex solution, the absorbance at 554 nm increases. This increase
in the absorbance is due to the decomposition of phenolphthalein- β-CyD inclusion com‐
plex by displacement of ibuprofen by phenolphthalein. This phenomenon indicates com‐
petition of the ibuprofen with phenolphthalein in the formation of inclusion complex
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with β-CyD. The amount of increase in the absorbance at 554 nm was found to be pro‐
portional with the ibuprofen concentration over a certain concentration range.
Figure 8. Absorption spectra for 4.8 ×10-5 mol L-1 phenolphthalein at pH 10.5 in the presence of (a) 1.0×10-4 mol L-1 β-
CyD and 2.0×10-4 mol L-1ibuprofen, (b) 1.0×10-4 mol L-1 β-CyD and (c) in the absence of β-CyD and ibuprofen. [Reprint‐
ed from Afkhami A, Madrakian T, Khalafi L. /Anal. Lett, 2007; 40 2317-2328 with permission from Taylor & Francis.]
Color change chemical sensors of CyD derivatives carrying dyes such as nitrophenol [35] and
alizarin yellow [36] were reported that relies on direct measurements of some analytes.
Also there is an example of color and spectral change of metal ion-indicators that affect‐
ed by β-CyD. Recently it has been demonstrated that the addition of β-CyD to the solu‐
tion containing the complex of calcium and magnesium with Eriochrome Black T (EBT)
caused decomposition of the 1:1 metal complex and increase in EBT concentration in sol‐
ution due to the formation of EBT-β-CyD inclusion complex. At a given pH, the values
of metal ion conditional formation constant (K'f) decreased by increasing β-CyD concen‐
tration based due to the formation of an inclusion complex between the desired form of
EBT and β-CyD. The amount of decrease in K'f with increasing β-CyD concentration and
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the color changes due to complex decomposition depends on the stability of the inclu‐
sion complex between EBT and β-CyD [37].
There is a large volume of published studies reporting the affinities and even selective affinity
of secondary hydroxyl side of CyDs for metal ion binding and complexation [38]. This
complexation ability improves considerably by structural and functional groups modification.
The secondary hydroxyl groups are deprotonated and coordinated to bind Pb(II) ions forming
a hexadecanuclear lead(II) alkoxide [39]. Two amino groups introduced on the primary
hydroxyl side of β-CyD can chelate a platinum ion [40]. 6-amino-glucopyranose analogue of
β-CyD had binding affinity for metal ions with Cs+ selectivity [41]. In 2010, Pitchumani et al.
reported a per-6-amino-β-CyD as a supramolecular host and p-nitrophenol as a spectroscopic
probe as a novel colorimetric and ratiometric sensor for transition metal cations, Fe3+ and
Ru3+ in water. Binding of these cations causes an appreciable change in the visible region of
the spectrum which can be detected by naked-eye and is insensitive to other metal ions namely
Ag+, Cu+, Mn2+, Fe2+, Cu2+, Zn2+, Cd2+, Hg2+, Pb2+, Cr3+, La3+ and Eu3+. The color change and
consequent sensing ability is significant at equimolar ratio of host and guest and also at very
low concentration [42].
Figure 9. The spectra of Ca-EBT complex (1.0 ×10-3 mol L-1 Ca2+ and 4.0 × 10-5 mol L-1 EBT) in the presence of (a) 0.0, (b)
3.0 × 10-3, (c) 6.0 × 10-3, (d) 9.0 × 10-3, (e) 1.2 × 10-2 and (f) 1.5 × 10-2 mol L-1 of β-CyD at pH 9.5. [Reprinted from Afkha‐
mi A, Khalafi L. / Supramol. Chem., 2008; 19 579-586 with permission from Taylor & Francis.]
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Figure 10. UV–Vis spectra of per-6-amino-β-CyD/p-nitrophenol (5×10−5 M) upon addition of Ru3+ (5×10−6M to 5×10−5
M). [Reprinted from Suresh P. Abulkalam Azath I, Pitchumani K. / Sens. Actuators, B 2010; 146 273-277 with permis‐
sion from Elsevier Science.]
Numerous studies have attempted to explain the possibility of incorporation of CyDs and
modified CyDs in the structures of ternary complexes as ligand. In some of them the whole
complex act as a guest and the metal ion has no direct contact with CyD [43]. In some other
complexes the CyD appears as a coordinating ligand [44-49]. For example the Imidazole-
appended β-CyD forms a ternary complex with a Cu2+ ion and l-tryptophanate [50]. The 6-
amino and imidazolyl groups of the host molecule and the carboxyl and amino groups of l-
tryptophanate are coordinated to the Cu2+ ion.
Moreover the cavity microenvironment of CyDs may alter the rate constant of reactions for
the guest molecules depend on the reaction, substrate and the differences between cavity and
solvent environments [51-53]. The changes in reaction rate cause to spectral time profile of the
substrate and may be applicable in selective kinetic measurement of substrates and their
recognition [54, 55].
2.4. Luminescence based molecular recognition
CyD inclusion is a means for protection of an excited state luminescent guest from the solvent
environment that frequently shows a marked increase of luminescence due to increase in
quantum yield and lifetime [56]. It have been mentioned even in some textbook that addition
of CyD in solution is an efficient way in attaining the room temperature phosphorescence. This
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effect is usually much larger than that observed in absorption, and has therefore been used
more efficiently and sensitively for luminescencing substrates. 6-bromo-2-naphthol is a good
example that exhibited room temperature in the presence of β-CyD owing to protection from
O2 quenching in a nondeoxygenated solution, although nitrogen purging increased the
emission intensity 13-fold [57].
For 2-chloronaphthalene solutions containing both d-glucose and α-CyD, the room-tempera‐
ture phosphorescence of 2-chloronaphthalene has been observed. The 2:1 inclusion complex
is responsible for the room-temperature phosphorescence. The quantum yield of the room-
temperature phosphorescence from the 2:1 inclusion complex has been determined to be 19%
of alcoholic solution at 77 K. When KI is added an enhancement is observed in phosphores‐
cence intensity due to the formation of a ternary inclusion complex with iodide. Also the
intensity reduction at higher concentrations of KI seems to be due to the formation of a
nonphosphorescent ternary inclusion complex containing two iodides [58]. The notion of
“turn-on” fluorescent sensor is used for this molecular recognition mechanism.
For the crown ether fluoroionophore/ β-CyD complex, the dimerization of the fluoroionophore
inside the β-CyD is found to be selectively promoted by alkali metal ion binding, thereby
resulting in metal-ion-selective pyrene dimer emission in water. This supramolecular function
is successfully utilized in the design of a podand fluoroionophore/β-CyD complex for sensing
toxic lead ion in water [59, 60].
O
O
O
O
O
H
N
O
OO
O
O
O
H
N
OOO
O
O
O
H
N
O
+K+
K+
-CyD
Figure 11. Response mechanism of benzo-15-crown-5 fluoroionophore /γ-CyD complex for K+ in water.
A further interesting application of fluorescence spectroscopy is its potential enantioselectiv‐
ity. Chiral discrimination has been demonstrated for CyD inclusion of camphorquinone [61].
The measurement of fluorescence anisotropy has been proposed as a method to determine the
enantiomeric composition of samples [62].
As well as UV-Visible spectroscopy; competition of desired analyte with CyD-bonded or
dissolved fluorophore yields a significant change in the fluorescence signal that will be useful
in molecular recognition. Various “turn-off” fluorescent chemical sensors, in which fluores‐
cence intensity was decreased by complexation with guest molecules, were reported.
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Figure 12. The mechanism of action for a turn-off fluorosensors.
A comprehensive example molecular recognition based on both decrease and increase in
fluorescence intensity is the dansyl bonded CyD with diethylenetriamine spacer (CyD-dien-
DNS) which have been reported by Corradini et al. In the presence of lipophilic organic
molecules, CyD-dien-DNS showed sensing properties due to competitive inclusion of the
guest and “in-out” movement of the dansyl group. CyD-dien-DNS was found also to be a
fluorescent chemosensor for copper(II) ion, with a linear response and good selectivity,
suggesting that a more flexible conformation of the linker and the presence of additional
binding sites allow binding of the metal ion by the amino and sulfonamidate groups.
Figure 13. Spectral change of dansyldiethylenetriamine modified cyclodextrin in the presence of copper ion. [Reprint‐
ed with permission from Corradini R, Dossena A, Galaverna G, Marchelli R, Panagia A, Sartor G. / J. Org. Chem., 62,
6283 (1997). Copyright 1997, American Chemical Society.]
The CyD-dien-DNS copper(II) complex was shown to behave as a chemosensor for bifunc‐
tional molecules, such as amino acids. In fact, upon addition of alanine, tryptophan, and
thyroxine, the negligible fluorescence intensity of Cu(CyD-dien-DNS) complex was “switched
on”, with a response dependent on the amino acid side chain [63]. Fluorescent indolizine
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modified CyD were studied in aqueous solution to evaluate their potentialities as molecular
chemosensors for volatile organic compounds (VOCs) such as adamantanol, benzene, toluene,
phenol and p-cresol as guest. The formation constant values measured using a spectral
displacement method and also some specific algorithm treatments are reported for their
quantitative analysis. [64, 65]. Some phenylseleno derivatives of CyD have been synthesized
as chiral molecular sensors. These modified cyclodextrins can recognize both the size and
chirality of the guest molecules despite of this fact that their stability constants with aliphatic
alcohols are generally smaller than those for native β-CyD [66].
Moreover some chiral amino acid modified CyDs have been synthesized as chiral molecular
sensors. N-dansyl-L-Phe-modified β-CyD showed high D-selectivity for norbornane deriva‐
tives and N-dansyl-D-Phe-modified β-CyD showed high L-selectivity for menthol [67]. Time-
resolved fluorescence studies showed that the fluorescence of the dansyl group was completely
quenched in the ternary complexes formed, and that the residual fluorescence was due to
uncomplexed ligand. The enantioselectivity in response was found to be due to the formation
of diastereomeric ternary complexes [68,69]. Fluorophore-amino acid-CyD were synthesized
and characterized as fluorescent indicators of molecular recognition [70]. A novel boronic acid
fluorophore 1/β-CyD complex sensor for sugar recognition in water has been designed [71].
2.5. Recognition of toxins based on spectral changes
There are also some successful applications of CyDs based spectral changes which have been
used for the recognition of biologically important toxins.
Cyanotoxins are potent toxic compounds produced by cyanobacteria during algal blooms,
which threaten drinking water supplies. These compounds can poison and kill animals and
humans. The host−guest interactions of CyDs with problematic cyanotoxins were investigated
to demonstrate the potential application of CyDs for the removal of these toxins from drinking
water or applications related to their separation or purification. The complexation of these
cyanotoxins with CyDs was monitored by nuclear magnetic resonance (NMR). The observed
changes in chemical shifts for specific protons and competitive binding experiments demon‐
strate a 1:1 inclusion complex between γ-CyD and microcystins and nodularin, and the results
suggest that CyD-type substrates are useful hosts for their complexation [72].
The fluorescence properties of the aflatoxins, as the most important mycotoxins, and the effect
of various CyDs on their fluorescence emission were studied. The complex formation constant
(Kf) of these compounds with β-CyD was chromatographically determined, and from the
results obtained, it has been concluded that Kf cannot be used alone to explain the fluorescence
increase [73].
An example of determination of biological toxins is a highly sensitive and rapid strategy for
characterizing aflatoxins and the cholera toxin based on capillary electrokinetic chromatogra‐
phy with multiphoton-excited fluorescence. The aflatoxins are a highly mutagenic multiple-
ringed heterocycles produced by aspergillus fungi and cholera toxin a-subunit is the catalytic
domain of the bacterial protein toxin from Vibrio cholera. The anionic carboxymethyl-β-CyD,
used to chromatographically resolve the uncharged aflatoxins, enhances emission from these
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compounds without contributing substantially to the background [74]. Also the determination
of aflatoxin B1 (AFB1) in wheat has been accomplished by enhanced spectrofluorimetry in the
presence of β-CyD. The method is based on the enhanced fluorescence of AFB1 by β-CyD in
10% (w/w) methanol–water solution. The adopted strategy combined the use of parallel factor
analysis (PARAFAC) for extraction of the pure analyte signal and the standard addition
method, for a determination in the presence of matrix effect caused by wheat matrix [75].
Figure 14. Contour plots (excitation–emission) for an original wheat sample and four AFB1 standard additions; (a) the
original sample, (b) plus 2.0 µg kg−1, (c) plus 3.8 µg kg−1, (d) plus 5.7 µg kg−1, (e) plus 7.4 µg kg−1. [Reprinted from Ha‐
shemi J, Asadi Kram G, Alizadeh N. / Talanta, 2008; 75 1075-1081 with permission from Elsevier Science.]
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2.6. Interaction and recognition of natural compounds
Finally the spectral change and interaction of some natural compounds such as alkaloids and
peptides with CyDs is discussed.
Complex formation of the glutathione and some of its derivatives with bridged β-CyD such
as 2,2′-diseleno-bridged β-CyD were determined by UV-Vis. absorption and 1H-NMR
spectroscopy [76]. Polymerization of the amyloid beta-peptide (Abeta) has been identified as
a major feature of the pathogenesis of Alzheimer's disease (AD). Inhibition of the formation
of these toxic polymers of Abeta has emerged as an approach for developing therapeutics for
AD. NMR and circular dichroism (CD) spectra were used to investigate the interaction between
CyD and Abeta. CD spectral analyses show that β-CyD inhibits the aggregation of Abeta.
Analysis of the one-dimensional proton NMR spectra of the mixture of Abeta with β-CyD
clearly indicates that there are chemical shift changes in the aromatic ring and the methyl
groups in the peptide [77].
A series of CyDs –cinchona alkaloid inclusion complexes were prepared from β-CyD and some
of its derivaties and four cinchona alkaloids, and their inclusion complexation behavior was
investigated by means of fluorescence, UV/Vis and 2D NMR spectroscopy. The results showed
that the cinchona alkaloids can be efficiently encapsulated in the CyD cavity in an acidic
environment and sufficiently released in a neutral environment, which makes these CyD
derivatives the potential carriers for cinchona alkaloids [78,79]. Using colorimetry and 1H-
NMR and UV spectroscopy, together with solubility methods,the interaction of natural and
hydroxylpropylated CyDs with xanthine, theophylline, theobromine, and caffeine in aqueous
solution have been studied [80].
Combination of the spectrophotometric methods and some separation methods such as capilla‐
ry electrophoresis (CE) and micellar electrokinetic chromatography (MEKC) in the presence of
CyDs have been used successfully for the quantitative analysis of natural alkaloids [80,81].
3. Conclusion
CyDs are a versatile tool in the molecular recognition and sensing. Formation of inclusion
complex cause to some spectral changes which have been used successfully for the study of
host-guest interactions. Additionally the desired spectral changes as the results of complex
formation have been used for promote analyte detection and continue to inspire creative
applications. The most sensible spectral changes were reported for chemical and fluorescence
indicators. These considerable changes have been used for the study and better detection of
many absorbing and especially fluorescent species. Moreover many spectrochemically silent
organic and some inorganic compounds cause color/fluorescence change in CyD and indicator
solutions, because of their competition to form inclusion complex. These changes cause to
recognition of the target competitive hosts. On this basis some "indicator modified cyclodex‐
trin" in which indicator is linked to cyclodextrin via a spacer, was synthesized that change
color/fluorescence in response to the presence of molecules, ions and many biologically
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important compounds. The guest-induced changes that are roughly parallel to its binding
constants were used for molecule sensing. These are valuable for qualitative and quantitative
chemical analysis. Sensitivity and selectivity improved by appropriate designing of the dye
moiety or spacer.
Author details
Lida Khalafi1 and Mohammad Rafiee2
1 Department of Chemistry, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
2 Department of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS),
Zanjan, Iran
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... 8,9 Displacement of indicators in β-CD cavity by any other molecule results in significant color changes (spectral changes) that provide a basis for detection of many colorless molecules. 10 Although this phenomenon occurs in a much smaller world than the one we are habituated to, information about it can be conveniently transmitted to us via simple color change. Detection of colorless molecules based on such a reversible and fast color changes is an example of "molecular recognition" because it strongly depends on molecular shapes and structure. ...
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A laboratory experiment is described in which students measure the amount of cetirizine in allergy-treatment tablets based on molecular recognition. The basis of recognition is competition of cetirizine with phenolphthalein to form an inclusion complex with β-cyclodextrin. Phenolphthalein is pinkish under basic condition, whereas it's complex form with β-cyclodextrin is colorless. Addition of the cetirizine leads to release of phenolphthalein from the phenolphthalein-β-cyclodextrin inclusion complex that alters the solution color under basic conditions. The intensity of the color change is proportional to the cetirizine concentration. This simple experiment provides an opportunity for students to become familiar with the concepts of inclusion complexes and molecular recognition. Students also gain hands-on experience with a spectrophotometer and engage in plotting a calibration curve based on absorbance or color changes and determination of cetirizine. © 2015 The American Chemical Society and Division of Chemical Education, Inc.
... Nevertheless, the ionization of C7-OH on the A ring and the presence of neutral and mono-anionic species at pH 6.8 could affect the UV-vis absorbance spectra [37,39]. As a result of inclusion complex formation, physical properties of the guest molecule such as solubility, absorbance and fluorescence spectra can be considerably changed [47] For example, complex formation with ␤-CyD results in a bathochromic shift and decreased absorption intensity in the absorbance spectrum of rutin [48]. Complex formation with ␥-CyD resulted in a bathochromic shift (5 nm) in band I while there was no shift in band II. ...
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The binding abilities of α- and β-cyclodextrins (α-CD and β-CD) with some heterocyclic azo compounds (1,1'-(azodicarbonyl)dipiperidine (ADP) and azodicarboxylic dimorpholide (ADM)) were studied at different pHs (4, 7.4 and 10) by UV-Vis spectroscopy and square-wave voltammetry techniques. The association constants (Ki) and stoichiometries of the binding of these azo compounds with α-CD and β-CD were determined by using square-wave voltammetry technique. These bindings were formed with a stoichiometry of 1: 1 in solution. The solid samples, obtained from the mixtures (molar ratio of 1: 1) of these azo dyes and CDs in aqueous phase were analyzed by FT-IR spectroscopy and thermal analysis methods. Thermal analysis results showed that ADP and ADM formed the inclusion complexes with α-CD; however, the binding of the azo dyes with β-CD gave non-inclusion complexes.
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Complex formation of the glutathione peroxidase mimics 2,2 cent-ditelluro-bridged b-cyclodextrin (1) and 2,2 cent-diseleno-bridged b-cyclodextrin (2), with S-substituted dinitrophenyl glutathione (3) were determined by ultraviolet-visible (UV-Vis) absorption spectroscopy in phosphate buffer (pH 7.4) and (1)H-NMR spectroscopy. Molecular mechanics (MM2) modeling calculations were used to deduce a three-dimensional model for each complex. The dinitrophenyl (DNP) group of 3 appears to penetrate the cavity of b-cyclodextrin (b-CD) or 1, but it is located between the two secondary rims of 2. The complexes' stability constants (K(s)) from 19 to 37 degrees C, Gibbs free energy changes (DG degrees ), DH degrees and TDS degrees for 1:1 complexes of b-CD, 1 and 2 with ligand 3 as obtained from UV-Vis spectra were compared. The binding of 3 by the three cyclodextrin hosts generally decreased in the order of 1>2>b-CD. The binding ability of 3 by b-CD, 1 and 2 was discussed with regard to the size/shape-fit concept, the induced-fit interaction, and the cooperative interaction of the dual hydrophobic cavities. The binding ability of 1>2indicated that the length of linkage between two cyclodextrin units plays a crucial role in the interaction with 3.
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Offering comprehensive and up-to-date know-how in one compact book, an experienced editor and top authors cover every aspect of these important molecules from molecular recognition to cyclodextrins as enzyme models. Chapters include reactivity and chemistry, chromatography, X-ray, NMR plus other physicochemical methods, as well as model calculations, rotaxane and catenane structures, and applications in the pharmaceutical industry. The book also discusses other applications such as in the cosmetics, toiletries, textile and wrapping industries, agrochemistry, electrochemical sensors, and devices. A must for everyone working with these substances.
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The aqueous solubility of the pesticide 2,4-D was improved by inclusion complexation with α-CD. The formation of such inclusion compounds was studied via the phase-solubility diagram (solution state) and by DSC and HSM (solid state). 2,4-D presented a typical Bs Higuchi solubility curve, coprecipitating a 1:2 pesticide-α-CD complex. In order to obtain solid complexes, three processing methods were checked: kneading, coprecipitation and spray-drying. DSC and HSM showed that only the last two of these yielded true inclusion compounds. Chemical analysis also revealed that the stoichiometry of such solid complexes corresponds to a 2,4-D-α-CD ratio of 1:2.
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A γ-cyclodextrin (γ-CyD) derivative (1) having a pyrene moiety, connected through a 4,13-diaza-18-crown-6 ether moiety to γ-CyD, was synthesized and evaluated for guest binding and sensing properties. In aqueous solution, 1 existed as an association dimer in which the secondary hydroxyl sides faced each other to accommodate two pyrene moieties. Photo- induced electron transfer (PET) between the amino group and the excited pyrene moiety regulated the monomer fluorescence intensity of 1. The monomer- dimer equilibrium and the PET indicated that I may be used as a host capable of detecting guest complexation by changes in fluorescence intensity from the pyrene moiety. Deoxycholic acids were found to be good guests for detection by 1, and deoxycholic acid itself induced different fluorescence changes compared to the other deoxycholic acids. This indicated that 1 could recognize the position of the hydroxy groups on the steroidal framework. The azacrown part may participate in the guest selectivity for the deoxycholic acids by regulating the distance between the amino group and the pyrene moiety, modifying PET efficiency.
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The effect of SBE4-β-CD, a sulfobutyl ether derivative of β-cyclodextrin on the solubility and aqueous hydrolysis of the antitumor drug O6-benzylguanine (BG) was studied. SBE4-β-CD is an apparently parenterally safe anionic β-cyclodextrin derivative with superior solubilizing properties in water. BG has poor aqueous solubility and undergoes rapid hydrolysis to the poorly water soluble guanine. The stability of a parenteral BG formulation was studied after storage at 25, 37 and 50°C. Compared to the intrinsic solubility of BG (0.14 mg/ml, 25°C), 0.05 M SBE4-β-CD enhanced its solubility to 2.9 mg/ml at 25°C and 3.9 mg/ml at 50°C. Solubility data yielded binding constants (Kb) of 565 M−1 at 25°C and 342 M−1 at 50°C. The solubility of guanine was only slightly enhanced by SBE4-β-CD. Hydrolysis kinetics of BG were studied at 50°C over a pH range of 1–9 and the maximum stability was observed at pH 8-8.5. In the presence of 0.05 M SBE4-β-CD, hydrolysis was about 9.5-times slower at pH 1, 14.6-times slower at pH 6 and 10-times slower at pH 8. The effect of SBE4-β-CD concentration was studied at pH 2.2 and 4.8 at 50°C. Hydrolysis rate constants decreased with increasing SBE4-β-CD concentrations. A non-linear regression analysis of this data yielded Kb values of 311 and 270 M−1 at pH 2.2 and 4.8, respectively. A formulation containing 2.5 mg/ml of BG and 0.05 M SBE4-β-CD in a pH 8 phosphate buffer was stored in ampoules at 25, 37 and 50°C. Guanine production in the samples was measured since its low solubility (2.5 μg/ml) imposed a limitation on the shelf life. Guanine levels exceeded its apparent solubility after 1–2 months of storage at 50°C. At 37°C guanine levels were only 1.6 μg/ml after 343 days of storage whereas those at 25°C were negligible and below the limit of quantitation (approx. 0.1 μg/ml). The greater stability at room temperature may be attributed to the higher Kb value observed and greater intrinsic stability of BG in the complex.
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The crystal structure of the α-cyclodextrin–ferrocene (2 : 1) inclusion compound has been determined by an X-ray analysis which shows that the ferrocene molecule with approximate D5d symmetry is encapsulated by the dimer of the α-cyclodextrins in a tail-to-tail orientation and inclined by 42° relative to the six-fold axes of the α-cyclodextrins of the dimer.
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Inclusion compounds of a range of cyclopentadienyl (arene)iron(II) sandwich complexes, structurally related ferrocenes and (tetramethylcyclobutadiene) cobalt complexes with α-, β- or γ-cyclodextrin (α-, β-, γ-cd) have been prepared. Complexes bearing sterically demanding ring ligands were selectively included in β- or γ-cd. X-Ray crystallographic studies of seven new α-cd adducts demonstrate that the molecular packing of the α-cd macrocycles in channel-type structures depends on the size and shape of the guest complexes. Crystal structure determinations of two of the β-cd adducts reveal a channel type structure similar to that of the corresponding α-cd adducts, although in these cases the exact orientation of the guest molecules could not be determined due to disorder.
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Rank annihilation factor analysis (RAFA) was used to the spectrophotometric studies of acid–base equilibria in the presence of �-cyclodextrin (�-CD). When the conditional acidity constants (�-CD concentration dependent acidity constant) acts as an optimizing object, and simply combined with the pure spectrum of acidic and basic forms, the rank of original data matrix can be reduced. The residual standard deviation (R.S.D.) of the residual matrix after bilinearization of the background matrix is regarded as the valuation function. The performance of the method has been evaluated by using synthetic data. Spectrophotometric studies of neutral red, 4-nitrophenol and 4-nitrocatechol in the presence of varying amounts of �-CD are used as experimental model systems and the amounts of conditional acidity constants and formation constants of the inclusion complexes of �-CD with acidic and basic forms are calculated. The conditional acidity constants increased by increasing �-CD concentration for all three investigated acids. The calculated stability constants show that 4-nitrophenolate, 4-nitrocatecholate anions and basic form of neutral red (neutral form) form more stable inclusion complexes with �-CD than their acidic forms.
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In this paper application of some functionalized cyclodextrins with ability of molecular recognition are described. Combination of size selectivity in cyclodextrins with some selective analytical response produces a molecular sensor for selective chemical, pharmaceutical and environmental analysis.