Antioxidant and antihyperlipidemic activity of Hibiscus sabdariffa Linn. leaves and calyces extracts in rats

Department of Pharmacognosy and Phytochemistry, Prin K M Kundnani College of Pharmacy, Cuffe Parade, Mumbai 400 005, India.
Indian journal of experimental biology (Impact Factor: 0.84). 05/2009; 47(4):276-82.
Source: PubMed


Antioxidant and antihyperlipidemic activities of the extracts of leaves and calyces of Hibiscus sabdariffa were investigated by studying their in vitro inhibitory activity on lipid peroxidation and in vivo effects on cholesterol induced hyperlipidemia. Highest antioxidant activity was exhibited by ethanolic extract of calyces followed by ethanolic extract of leaves followed by aqueous extract of leaves of H. sabdariffa. In cholesterol induced hyperlipidemic model, groups of rats treated with extracts of calyces and leaves of H. sabdariffa showed a significant decrease in the serum TC, LDL-C, VLDL-C, TAG values alongwith an increase in serum HDL-C levels. The treated groups also showed significant decrease in the atherogenic index, LDL-C: HDL-C risk ratios, and in the levels of SGOT, SGPT and ALP activities compared to cholesterol induced hyperlipidemic control group. Significant antihyperlipidemic activity was shown by ethanolic extract of calyces, followed by ethanolic extract of leaves. It was observed from the histopathological findings that rats fed with H. sabdariffa extracts showed decrease in granular degeneration caused by cholesterol feedings. Results suggest that the ethanolic extracts of calyces and leaves of H. sabdarifa containing polyphenols and flavanols possess significant antioxidant and antihyperlipidemic activities.

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    • "G The results from our study indicate that aqueous extract of Hibiscus sabdariffa Linn popularly known as " zobo " drink in our community has adverse effects on the body weight of the experimental animals. This is in contrast to the study by Ochani and D'mello [13], in which significant increase in the body weights of the experimental animals was observed in a pattern suggesting the influence of the administered dosage. This observation therefore questions the reported positive effect of Hibiscus sabdariffa on obesity. "
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    ABSTRACT: Hibiscus sabdariffa L. has been used traditionally as herbal medicine and has been documented to have a broad range of therapeutic effects. The present study aimed to investigate the effects of chronic administration of aqueous extract of flowers of Hibiscus sabdariffa on the histology of the kidney and some biochemical indices of renal function in male Wistar rats. Twenty (20) Wistar rats were randomly divided into four (4) groups of five rats each. The extract was administered orally in doses 200, 500, and 800 mg/kg body weight for 21 days. The kidney was harvested and processed histologically and blood samples were taken for biochemical assays. The histological results showed dose dependent pathological states and the biochemical analysis revealed a dose dependant variation in renal indices. These results suggest that chronic administration of aqueous extract of Hibiscus sabdariffa may be toxic to the kidney.
    Full-text · Article · Nov 2015
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    • "After four weeks' treatment of H. sabdariffa leaf extract in rats, significant reductions of serum cholesterol, serum triglyceride, serum LDL (low-density lipoprotein ) and serum VLDL (very-low-density lipoprotein) levels were observed in cholesterol-induced hyperlipidemic rats, suggesting its antihyperlipidemic effects (Gosain et al., 2010). The anti-atherosclerotic activity is explained by the inhibition of LDL oxidation and foam cell formation via LXRa/ABCA1 pathway (Chen et al., 2013; Ochani & D'Mello, 2009). The anti-proliferation effects of H. sabdariffa leaf extract on LNCaP cells (human prostate cancer cells) may involve both intrinsic (Bax/cytochrome c-mediated caspase 9) and extrinsic (Fas-mediated caspase 8/t-Bid) apoptotic pathways (Lin et al., 2012). "
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    ABSTRACT: Highlights: • Phytochemical determination in the leaves of Hibiscus sabdariffa. • The antioxidant and anti-inflammatory activity of Hibiscus sabdariffa leaves. • The exploration of 5-(hydroxymethyl)furfural for quality control. • Chemical profile comparison between different Hibiscus sabdariffa populations. Abstract: A liquid chromatography-mass spectrometry method was developed for the simultaneous separation, and determination of natural compounds including phenolic acids and flavonoids in the leaves of Hibiscus sabdariffa. By analyzing the UV and MS data, and comparison with authenticated standards, 10 polyphenols including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, quercetin, kaempferol and their glycosides were identified together with 5-(hydroxymethyl)furfural. Major constituents in the leaves of 25 different populations from worldwide accessions were quantified and compared with each other. The total phenolic content of each accession was determined using Folin-Ciocalteu assay, ranging from 18.98 ± 2.7 to 29.9 ± 0.5 mg GAE /g. Their in vitro antioxidant activities were measured by ABTS radical cation decolorization assay, varying from 17.5 to 152.5 ± 18.8 μmol Trolox /g. After the treatment of Hibiscus sabdariffa leaf extract, the reduction of LPS-induced NO production dose-dependently in RAW 264.7 cell indicates the extract’s potential anti-inflammatory activity.
    Full-text · Article · Jun 2015 · Food Chemistry
    • "It is evident that hypercholesterolemia increases oxidative stress and consequently elevates lipid peroxides.[5] Many experimental reports has determined the antioxidant enzyme activities to assess the status of free radical damage or oxidative stress.[6789] Therefore, antioxidant enzymes and lipid peroxidation status were assessed in serum, liver and heart of rats to determine the effect of ambrex treatment on diet induced hyperlipidemia. "
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    ABSTRACT: Background:Ambrex is a polyherbal formulation which consists of Withania somnifera, Orchis mascula, Cycas circirnalis, Shorea robusta with amber.Objective:The present study was designed to explore the potential effects of ambrex on the antioxidant status in high fat diet fed rats and to investigate the possible mechanisms focusing on the gene expression involved in adipogenesis and inflammation in 3T3-L1 cell line.Materials and Methods:Male Wistar rats were divided into four groups (n = 6); Group A received normal diet, Group B received high fat diet for 30 days, Group C and D received high fat diet for 30 days and treated with ambrex (40 mg/kg b.w) and atorvastatin (10 mg/kg b.w) for successive 15 days respectively. This study also assesses the effect of ambrex on adipogenesis in 3T3-L1 adipocytes.Results:The serum total cholesterol and triglycerides were significantly decreased in ambrex treated hyperlipidemic animals when compared to untreated animals. The activities of catalase, superoxide dismutase and reduced glutathione were significantly augmented in the serum, liver, and heart of hyperlipidemic rats treated with ambrex when compared to control. Ambrex treated rats had significant reductions in malondiadehyde levels in the serum, liver and heart compared to untreated rats. In addition, we observed that treatment with ambrex resulted in a major inhibition of pre-adipocyte differentiation of 3T3-L1 cells in vitro by suppression of peroxisome proliferator activated receptor gamma, sterol regulatory binding proteins, tumor necrosis factor-α, inducible nitricoxide synthase, leptin, and upregulation of thioredoxin 1 (TRX1) and TRX2 mRNA expression.Conclusion:Therefore, ambrex may be a potential drug for treatment of hyperlipidemia and related disorders.
    No preview · Article · Apr 2014 · Pharmacognosy Magazine
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