New Fluorescence Microscopy Methods for Microbiology: Sharper, Faster, and Quantitative

Department of Molecular Biology, Princeton University, Princeton, NJ 08540, USA.
Current opinion in microbiology (Impact Factor: 5.9). 05/2009; 12(3):341-6. DOI: 10.1016/j.mib.2009.03.001
Source: PubMed


In addition to the inherent interest stemming from their ecological and human health impacts, microbes have many advantages as model organisms, including ease of growth and manipulation and relatively simple genomes. However, the imaging of bacteria via light microscopy has been limited by their small sizes. Recent advances in fluorescence microscopy that allow imaging of structures at extremely high resolutions are thus of particular interest to the modern microbiologist. In addition, advances in high-throughput microscopy and quantitative image analysis are enabling cellular imaging to finally take advantage of the full power of bacterial numbers and ease of manipulation. These technical developments are ushering in a new era of using fluorescence microscopy to understand bacterial systems in a detailed, comprehensive, and quantitative manner.

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    • "Most of the singlecell gene expression studies in bacteria have been performed at the protein level (Elowitz et al., 2002; Lahav et al., 2004; Cai et al., 2006; Suel et al., 2006; Yu et al., 2006). Recent reviews provide a comprehensive picture of the state-of-the-art in this area (Levsky and Singer, 2003; Miyashiro and Goulian, 2007; Fraser and Kaern, 2009; Larson et al., 2009) including different aspects of fluorescence microscopy in microbiology (Gitai, 2009; Fero and Pogliano, 2010). "
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    • "[cs.CV] 18 Jan 2011 cessing pipeline are due to the low resolution of the image stacks, due to movements of the cells over time, due to other obscuring cells and structures, and due to the low contrast between the DNA damage and the surrounding nucleus. An overview of current methods for the analysis of fluorescent microscopy images can be found in [7] and references therein. "
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