Controlling the selection stringency of phage display using a microfluidic device

Neuroscience Research Institute, University of California, Santa Barbara, CA93106, USA.
Lab on a Chip (Impact Factor: 6.12). 05/2009; 9(8):1033-6. DOI: 10.1039/b820985e
Source: PubMed


We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.

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Available from: Frank V Cochran, Jan 20, 2014
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    • "Recently a new cell-surface biopanning method based on the microfluidics technology was proposed. The accurate control of washing stringency in the microfluidic magnetic separator (MMS) circumvents cell loss and allows efficient removal of weakly or nonspecifically-bound phages [31]. The small numbers of cells required constitutes an advantage, rendering MMS a promising tool for the discovery of biomarkers from patient samples. "
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    • "The micromagnetic separation (MMS) chip was fabricated and assembled according to a process previously described by our group (11–13). Briefly, the device was fabricated on borosilicate glass substrates with 25 -µm-thick double-coated tape (3M). "
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