Pelagibius litoralis gen. nov., sp nov., a marine bacterium in the family Rhodospirillaceae isolated from coastal seawater

Open and Tropical Ocean Research Division, Korea Ocean Research and Development Institute, Ansan 426-744, Republic of Korea.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 05/2009; 59(Pt 4):818-23. DOI: 10.1099/ijs.0.002774-0
Source: PubMed


A Gram-negative, strictly aerobic, slightly curved rod-shaped bacterial strain, designated CL-UU02(T), was isolated from coastal seawater off the east coast of Korea. 16S rRNA gene sequence analysis revealed a clear affiliation of this novel strain with the family Rhodospirillaceae. Strain CL-UU02(T) formed a robust cluster with the type strains of species of the genus Rhodovibrio at 16S rRNA gene sequence similarity levels of 89.9-90.4 %. Strain CL-UU02(T) shared no more than 89 % 16S rRNA gene sequence similarity with the type strains of other species in the family Rhodospirillaceae. Strain CL-UU02(T) was able to grow in the presence of 2-6 % sea salts, and grew optimally at 28-30 degrees C and pH 7-8. The DNA G+C content of strain CL-UU02(T) was 66.3 mol%. On the basis of phylogenetic analyses and chemotaxonomic and physiological data, strain CL-UU02(T) is considered to represent a novel species of a new genus in the family Rhodospirillaceae, for which the name Pelagibius litoralis gen. nov., sp. nov. is proposed. The type strain of Pelagibius litoralis is CL-UU02(T) (=KCCM 42323(T)=JCM 15426(T)).

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    • "However, the Rhodobacteraceae-affiliated organisms that were most abundant in our sequencing dataset could not be classified, indicating that they belonged to novel clades. Rhodospirillaceae-related organisms were also isolated from deep-sea sediments (Lai et al., 2009), however, the clade that seemed to be most abundant along the Antarctic Polar Front, Pelagibius, was to our knowledge only described in seawater samples (Choi et al., 2009). The clade NOR5/OM60 contains Congregibacter litoralis, a marine aerobic, anoxygenic phototroph (Fuchs et al., 2007) and seems to preferably occur in surface waters and coastal sediments with high productivity (Lenk et al., 2011; Spring et al., 2013; Yan et al., 2009). "
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    • "Studies on the molecular mechanisms used by these microorganisms to adapt to osmotic stress have been reported both at genetic and biochemical levels for bacteria such as Escherichia coli [13], Bacillus subtilis [14], Listeria monocytogenes [15], Staphylococcus xylosus [16], Sinorhizobium melitoti [17] and Arthrobacter globiformis [18]. Within the Rhodospirillaceae family belonging to Alphaproteobacteria, many genera include halophilic strains isolated from marine environments, such as Thalassobaculum [19], Nisaea [20], Thalassospira [21], Rhodospira, Roseospira, Rhodovibrio, Rhodospirillum [22], Marispirillum [23], Oceanibaculum [23] and Pelagibius [24]. It also includes halotolerant strains isolated from sludge, cavity within white rock and saline spring, as exemplified with strains from the genera Caenispirillum [25], Inquilinus [26], Constrictibacter [27] and Tistlia [28]. "
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    • "The family Rhodospirillaceae, of subgroup 1 of the class Alphaproteobacteria, comprises 22 genera at the time of writing ( html#Rhodospirillaceae). Members of several novel genera of this family have been isolated recently from various environments, such as Caenispirillum bisanense from sludge of a dye works (Yoon et al., 2007), Fodinicurvata fenggangensis and F. sediminis from a salt mine (Wang et al., 2009), Marispirillum indicum from a deep-sea environment (Lai et al., 2009a), Nisaea denitrificans and N. nitritireducens from surface seawater (Urios et al., 2008), Oceanibaculum indicum from deep seawater (Lai et al., 2009b), Pelagibius litoralis from seawater (Choi et al., 2009), Thalassobaculum litoreum from coastal seawater (Zhang et al., 2008) and Telmatospirillum siberiense from a mesotrophic fen (Sizova et al., 2007). The wide distribution and metabolic diversity (such as heterotrophism or phototrophism) of the family Rhodospirillaceae suggest that its members may have an important role in such aquatic and marine environments. "
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